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Clapp, Tod R.; Stone, Leslie M.; Margolskee, Robert F.; Kinnamon, Sue C.

doi:10.1186/1471-2202-2-6

Cited by 216 publications

(73 citation statements)

References 32 publications

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“…1A, we detected all three subtypes of IP 3 Rs in WT taste bud lysates by Western blotting. Consistent with the previous data using in situ hybridization and RT-PCR (20,21), the expression of IP 3 R3 is predominant among the three subtypes of IP 3 R because the pan-IP 3 R antibody, which recognizes all types of IP 3 Rs at a similar level (22), detected the bands for IP 3 Rs in WT taste bud lysates but not in IP 3 R3-deficient taste bud lysates (Fig. 1A).…”

Section: Taste Bud Morphology and The Expression Of Taste-relatedsupporting

confidence: 91%

Abnormal Taste Perception in Mice Lacking the Type 3 Inositol 1,4,5-Trisphosphate Receptor

Hisatsune

Yasumatsu

Takahashi-Iwanaga

et al. 2007

Journal of Biological Chemistry

142

103

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Inositol 1,4,5-trisphosphate receptor (IP 3 R) is one of the important calcium channels expressed in the endoplasmic reticulum and has been shown to play crucial roles in various physiological phenomena. Type 3 IP 3 R is expressed in taste cells, but the physiological relevance of this receptor in taste perception in vivo is still unknown. Here, we show that mice lacking IP 3 R3 show abnormal behavioral and electrophysiological responses to sweet, umami, and bitter substances that trigger G-proteincoupled receptor activation. In contrast, responses to salty and acid tastes are largely normal in the mutant mice. We conclude that IP 3 R3 is a principal mediator of sweet, bitter, and umami taste perception and would be a missing molecule linking phospholipase C ␤2 to TRPM5 activation.Taste perception is a pivotal and primitive sensory system for survival in animals. By sensing taste, animals are provided with valuable information about foods (e.g. qualities and nature) and can choose the nutrient-rich foods necessary for living or avoid harmful and toxic substances. There are five taste categories (sweet, bitter, umami, sour, and salty), and recent studies have furthered our understanding of the molecular mechanisms of taste perception, especially for sweet, bitter, and umami tastes (1, 2).For perception of sweet, bitter, and umami taste, phospholipase C ␤2 (PLC␤2) 3 activation through G-protein-coupled receptor (sweet, T1R2 ϩ T1R3; umami, T1R1 ϩ T1R3; bitter, T2Rs) (1, 3-8) and the subsequent activation of PLC␤2 and transient-receptor potential receptor M5 (TRPM5) are necessary (8, 9), but the molecular mechanism by which PLC␤2 activation leads to TRPM5 in vivo is still unclear (2). Several reports have suggested the possible involvement of Ca 2ϩ , probably released from the intracellular stores, in the activation of TRPM5 in heterologously expressed cells (10 -14) and in taste cells (15); however this remains controversial (9). Because PLC␤2 activation actually leads to production of both IP 3 and diacylglycerol, it is an important issue to definitely determine, which is a major player for gustatory systems. To clarify whether IP 3 R is necessary for taste perception in vivo, we analyzed the taste signaling of IP 3 R-deficient mice in this study (16). We found that mice lacking IP 3 R3 showed altered taste recognition for sweet, bitter, and umami, whereas they were indistinguishable from wild-type (WT) mice in their recognition for salty and sour stimuli. However, they showed residual responses to high concentrations of sweets and bitter. Our data present the direct validation that IP 3 R3 is a key molecule in taste perception for sweet, bitter, and umami and also suggest the existence of IP 3 R3-independent taste signal transduction for recognition of high dose of these tastants. EXPERIMENTAL PROCEDURESMice-IP 3 R3-and IP 3 R2-deficient mice were generated as described previously (16), and the mice intercrossed with C57BL/6 mice at least twelve times were used. WT C57BL/6 mice were littermates or purchased from ...

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Section: Taste Bud Morphology and The Expression Of Taste-relatedsupporting

confidence: 91%

Abnormal Taste Perception in Mice Lacking the Type 3 Inositol 1,4,5-Trisphosphate Receptor

Hisatsune

Yasumatsu

Takahashi-Iwanaga

et al. 2007

Journal of Biological Chemistry

142

103

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“…For double labeling, we used the following antibodies: rabbit anti-ubiquitin carboxyl-terminal transferase (PGP 9.5) (Biogenesis, Bournemouth, U.K.), rabbit anti-CGRP (Chemicon), rabbit phospholipase C ␤2 [PLC ␤2; affinity-purified antibody directed against a carboxyl-terminal peptide; sc-206 (Santa Cruz Biotechnology; ref. 18)] and mouse anti-acetylated tubulin (Sigma). Mouse anti-acetylated tubulin (1:1,000) and rabbit anti-G␣gust (1:500) were applied as a mixture overnight at 4°C and reacted the next day with a mixture of secondary antisera with different fluorochromes.…”

Section: Methodsmentioning

confidence: 99%

Solitary chemoreceptor cells in the nasal cavity serve as sentinels of respiration

Finger

Böttger

Hansen

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

372

453

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Inhalation of irritating substances leads to activation of the trigeminal nerve, triggering protective reflexes that include apnea or sneezing. Receptors for trigeminal irritants are generally assumed to be located exclusively on free nerve endings within the nasal epithelium, requiring that trigeminal irritants diffuse through the junctional barrier at the epithelial surface to activate receptors. We find, in both rats and mice, an extensive population of chemosensory cells that reach the surface of the nasal epithelium and form synaptic contacts with trigeminal afferent nerve fibers. These chemosensory cells express T2R ''bitter-taste'' receptors and ␣-gustducin, a G protein involved in chemosensory transduction. Functional studies indicate that bitter substances applied to the nasal epithelium activate the trigeminal nerve and evoke changes in respiratory rate. By extending to the surface of the nasal epithelium, these chemosensory cells serve to expand the repertoire of compounds that can activate trigeminal protective reflexes. The trigeminal chemoreceptor cells are likely to be remnants of the phylogenetically ancient population of solitary chemoreceptor cells found in the epithelium of all anamniote aquatic vertebrates.

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“…Of them, the glutamateaspartate transporter, nucleoside triphosphate diphosphohydrolase 2 (NTPDase2), and epithelial amiloride-sensitive Na + channel are believed to be specific for type I cells (Lawton et al, 2000;Bartel et al, 2006;Vandenbeuch et al, 2008). Virtually all cells of type II express phospholipase Cb2 (PLCb2), the InsP 3 receptor type 3 (IP 3 R3), and the channel subunit TRPM5 (Clapp et al, 2001;Perez et al, 2002), although evidence exists that IP 3 R3 also operates in a subpopulation of type III cells (Clapp et al, 2004;DeFazio et al, 2006). In the CV papilla, the taste-specific G-protein gustducin and GPCR T1R3 are most probably expressed in separate subpopulations of type II cells (Kim et al, 2003;Shindo et al, 2008).…”

Section: Single-cell Profiling Of Rna Transcriptsmentioning

confidence: 99%

Functional expression of the extracellular-Ca2+-sensing receptor in mouse taste cells

Bystrova

Romanov

Rogachevskaja

et al. 2010

Journal of Cell Science

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Cited by 216 publications

References 32 publications

Abnormal Taste Perception in Mice Lacking the Type 3 Inositol 1,4,5-Trisphosphate Receptor

Abnormal Taste Perception in Mice Lacking the Type 3 Inositol 1,4,5-Trisphosphate Receptor

Solitary chemoreceptor cells in the nasal cavity serve as sentinels of respiration

Functional expression of the extracellular-Ca2+-sensing receptor in mouse taste cells

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