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Hutterer, Rudi; Parusel, Andreas B. J.; Hof, Martin

doi:10.1023/a:1020532817530

Cited by 37 publications

(27 citation statements)

References 13 publications

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“…Prodan is soluble in cyclohexane with an absorption maximum ν np (abs) equal to 29 240 cm −1 and an emission maximum ν np (em) equal to 24 938 cm −1 [41]. Since the maximum of the TRES of Prodan in the DPPC gel phase at 15 ns after excitation has been determined to be about 22 375 cm −1 the spectral shift with time after excitation ∆ν = ν(0) − ν(∞) for this system is rather small when compared to a total Stokes shift of about 3500 cm −1 of Prodan in phosphatidylcholine vesicles above the phase transition [35]. Assuming that the maximum of the more blue-shifted and intensive excitation band (27 778 cm −1 ) [42] is equal to ν p (abs), a ν(0) value of 23 476 cm −1 for Prodan in the DPPC gel phase is obtained.…”

Section: Discussionmentioning

confidence: 80%

“…The validity of this approach has been demonstrated in several recent publications [22][23][24], and summarised in a few reviews [28,35,36] phase bilayers is very slow; thus, hardly any red shift with time is detectable (Fig. The validity of this approach has been demonstrated in several recent publications [22][23][24], and summarised in a few reviews [28,35,36] phase bilayers is very slow; thus, hardly any red shift with time is detectable (Fig.…”

Section: Resultsmentioning

confidence: 95%

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Probing Ethanol-Induced Phospholipid Phase Transitions by the Polarity Sensitive Fluorescence Probes Prodan and Patman

Hutterer¹,

Hof²

2002

Zeitschrift Für Physikalische Chemie

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The emission behaviour of the two polarity sensitive probes Prodan and Patman in phospholipid vesicles was studied as a function of the concentration of ethanol. Comparing the spectral shifts in both the symmetric lipid 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) showing a phase transition from a normal to a fully interdigitated gel phase and the strongly asymmetric lipid 1-stearoyl-2-lauroyl-sn-glycero-3-phosphatidylcholine (C(18):C(12)-PC) favouring a mixed interdigitated gel phase we show that the huge red shifts of Prodan in presence of higher ethanol concentrations cannot be easily attributed to a specific lipid phase transition. Rather, probe relocation and a pronounced increase in solvent relaxation (SR) as monitored by time-resolved emission spectra (TRES) in presence of ethanol contribute to the large shifts observable in both lipid systems in case of Prodan. While Patman exhibits a red shift caused by increased SR due to the ethanol induced formation of a fully interdigitated phase in DPPC, hardly any shift occurs in C(18):C(12)-PC, which is supposed not to undergo an ethanol-induced phase transition.

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Section: Discussionmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 95%

Probing Ethanol-Induced Phospholipid Phase Transitions by the Polarity Sensitive Fluorescence Probes Prodan and Patman

Hutterer¹,

Hof²

2002

Zeitschrift Für Physikalische Chemie

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“…Ph (1,2) (t) and Ph (2,1) (t) show a initially rising multiexponential evolution because the emitting states 2 and 1 are the transient excited states ͑i.e., they are pumped through the primary excited states 1 and 2, correspondingly͒. Similarly, the fluorescencedetected time-dependent excited-state order parameters ͗D 00 ( j) ͘ ex,k→ (t) of rank jϭ0, 2,4,6,8,10,12 are shown in Figs. 1͑d͒ and 1͑e͒ are shown the time evolutions of the first two terms in expansion ͑33͒.…”

Section: Angular Dependence Of the Kinetic Rates Illustrative Exmentioning

confidence: 98%

“…1, if the kinetic rates k 12 (␤) and k 21 (␤) are orientation independent ones, i.e., k 12 (␤)ϵk 12 and k 21 (␤)ϵk 21 , the anisotropic terms in ͑33͒ disappear and the evolution of Ph (k,) (t) is bi-exponential. As has been pointed out in Ref.…”

Section: Angular Dependence Of the Kinetic Rates Illustrative Exmentioning

confidence: 99%

Fluorescence depolarization in organized media. Two-excited-state reactions controlled by orientation-dependent kinetic rates. I. Theory

Fisz

Budziński

2001

The Journal of Chemical Physics

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Articles you may be interested inTime-dependent density functional theory excited state nonadiabatic dynamics combined with quantum mechanical/molecular mechanical approach: Photodynamics of indole in water J. Chem. Phys. 135, 054105 (2011); 10.1063/1.3622563 Intramolecular charge transfer of 4-(dimethylamino)benzonitrile probed by time-resolved fluorescence and transient absorption: No evidence for two ICT states and a π σ reaction intermediate A model describing fluorescence depolarization of fluorophores undergoing the two-ground-and two-excited-state reactions in ordered systems, is discussed. It is assumed that the rates for state-to-state kinetic relaxation process are dependent on angular orientation of fluorophores, and that this process is controlled by fluorophore-matrix ground-and excited-state aligning interactions and by rotational dynamics. The evolution of the two-excited-state system is described by the equation of motion composed of a set of two differential equations for the Green functions, coupling state-dependent molecular ordering and rotational dynamics with the orientation-dependent kinetic relaxation. A method of solving the motion equation is outlined. The main properties of the solution to motion equation and its information content on angular alignment and potential-restricted rotational dynamics of fluorophores, are discussed in detail. An illustrative example manifesting the effect of orientation-dependent excited-state reversible interconversion process on fluorescence depolarization, is presented. Two experimental arrangements enabling the recovery of the model parameters are presented and discussed. Possible applications of the model to different cases important from the experimental point of view, are indicated and discussed.

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“…Such molecules are of undoubted commercial interest as laser dyes and fluorescent probes due to their high sensitivity to the microenvironment [1]. The fluorescing organic molecules with the simultaneous presence of donor and acceptor groups are widely used in biochemical investigations as probes for studying the physical and chemical properties of solvents, surfaces of different physical natures, large biological molecules, membranes, cells, and so on [3]. In this regard, interesting objects for investigations are prodan (6-propionyl-2-dimethylamine-naphthalene) and its derivatives.…”

Section: Introductionmentioning

confidence: 99%

Role of geometrical prodan structures in the determination of possible centers of hydrogen bond formation

et al. 2008

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535.37 V. Ya. Artyukhov, and B. V. Korolev Using the method of intermediate neglect of differential overlap (INDO), quantum-chemical investigations of geometrical prodan structures with different rotation and deflection angles of methyl groups in the dimethylaminogroup of the molecule are performed. The charge distribution is studied for different conformations of the molecule. The possible centers of prodan interaction with proton-donor solvents are estimated. It is demonstrated that in addition to the oxygen and nitrogen atoms, the carbon atoms of the hydrocarbon chain and the aromatic prodan ring can interact with the solvent.

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Untitled

Cited by 37 publications

References 13 publications

Probing Ethanol-Induced Phospholipid Phase Transitions by the Polarity Sensitive Fluorescence Probes Prodan and Patman

Probing Ethanol-Induced Phospholipid Phase Transitions by the Polarity Sensitive Fluorescence Probes Prodan and Patman

Fluorescence depolarization in organized media. Two-excited-state reactions controlled by orientation-dependent kinetic rates. I. Theory

Role of geometrical prodan structures in the determination of possible centers of hydrogen bond formation

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