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Abstract

Acute and chronic inflammation-induced expression of sialyl LewisX has already been shown to occur on alpha1-acid glycoprotein. We now demonstrate that this phenomenon is not restricted to alpha1-acid glycoprotein but also occurs on two other acute-phase proteins. ie on alpha1-antichymotrypsin and on haptoglobin. The level of expression of sialyl LewisX on these proteins was lower than on alpha1-acid glycoprotein, in all likelihood because alpha1-acid glycoprotein is the only acute-phase protein containing tetraantennary glycans. No expression of sialyl LewisX was detectable on alpha1-protease inhibitor, a protein with a high diantennary glycan content. Non-sialylated LewisX was not detectable on these major acute-phase proteins in any of the conditions studied. This indicates that the majority of the a3-linked fucose residues are present as sialyl LewisX on alpha1-acid glycoprotein, alpha1-antichymotrypsin and haptoglobin. The absolute contribution to the total phenotype in plasma of protein containing this determinant in a multivalent form was highest for alpha1-acid glycoprotein. This leads us to propose that alpha1-acid glycoprotein is, among the acute-phase proteins studied, the one with the highest potential for interference with the extravasation of leukocytes by binding to the selectins.

Tumor Necrosis Factor α Increases the Expression of Glycosyltransferases and Sulfotransferases Responsible for the Biosynthesis of Sialylated and/or Sulfated Lewis x Epitopes in the Human Bronchial Mucosa

“…These results are in agreement with the observations that inflammation, and particularly TNF-␣, changes ␣3-fucosylation and induces sialyl-Lewis x expression on several glycoproteins (23,51,52). ␣1,3-Fucosyltransferases are required after ␣2,3-sialyltransferases in the generation of the sialyl-Le x determinants.…”

The Serum Glycome to Discriminate between Early-Stage Epithelial Ovarian Cancer and Benign Ovarian Diseases

“…The increase of the monofucosylated triantennary trisialylated N-glycan at m / z 3776.9, carrying a sialyl Lewis X antigen, that was reported here is a common feature in several cancers and inflammatory conditions: prostate metastatic cancer [43], lung cancer [44], breast cancer [27], acute pancreatitis, and sepsis [29]. The sialyl Lewis X antigen is carried by acute-phase glycoproteins that circulate in serum of patients suffering from inflammatory conditions: α -1 acid glycoprotein, haptoglobin, and α 1-antichymotrypsin [45]. In addition, the upregulation of the corresponding glycosyltransferases was also measured in the serum of ovarian cancer patients: ( α 1-3) fucosyltransferase [46], ( β 1-4) galactosyltransferase [47], and ( α 2-6) sialyltransferase [48, 49].…”

The Acute Phase Protein α1-Acid Glycoprotein: A Model for Altered Glycosylation During Diseases

“…AGP fucosylation does not significantly change after one year of anti-inflammatory therapy, and it was hypothesized that the persistent high level of AGP-fucosylated residues may reflect a basic pathogenic inflammatory status that persists during the short-term of the clinically apparent disease activity [135]. Interestingly, this increase in fucosylation is not exclusive to AGP but has also been described for other acute phase proteins, including haptoglobin [136] and 1-antichimotrypsin [137]: in both proteins the majority of the (1 )3-linked fucose were present as sLe X conformation. AGP's glycan pattern modification has also been investigated in other autoimmune diseases.…”