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Jin, Takashi

doi:10.1023/a:1024506714332

Cited by 37 publications

(21 citation statements)

References 20 publications

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“…The limit of detection for acetylcholine was found to be 6.3 Â 10 À8 g/mL with a signal-to-noise ratio of 3:1. This was 1-3 orders of magnitude higher than what was observed for artificial receptorbased systems [2,4]. It was also comparable to the acetylcholine detection limit achieved by acetylcholine enzymebased sensors [6,8].…”

supporting

confidence: 56%

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Wei¹,

Wei²,

Kang³

et al. 2009

Electrophoresis

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A novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2'-bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3 x 10(-8) g/mL with a signal-to-noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912+/-58 microg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92-106%. The coefficients of variation for intra-day and inter-day reproducibility were equal to or less than 4.9 and 6.8%, respectively.

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supporting

confidence: 56%

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Wei¹,

Wei²,

Kang³

et al. 2009

Electrophoresis

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“…[1,2] As analytes of prime interest, we have selected choline-and carnitine-derived substrates. The neurotransmitter, acetylcholine, in particular, has an ample and recent history in relation to sensors based on calixarenes [11][12][13][14] and related macrocyclic host molecules. [20,21] As established by UV and 1 H NMR titrations and fluorescence quenching experiments, DBO forms a 1:1 inclusion complex with CX4 in aqueous solution over a large pH range, with binding constants on the order of 10 3 M -1 .…”

Section: Discussionmentioning

confidence: 99%

“…The intensity increase, which depends on the actual host/guest concentrations, exceeded a factor of 4 at pD 2.4, which is superior to previous calixarene-based fluorescent sensor systems. [12][13][14] Note, in particular, that the DBO-based sensor system operates, in principal, over the entire pD range in neat water, i.e., it does not require the use of organic co-solvents and is not restricted to alkaline media like the original resorcinarene system [20] (see Introduction).…”

Section: Discussionmentioning

confidence: 99%

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Fluorescence Regeneration as a Signaling Principle for Choline and Carnitine Binding: A Refined Supramolecular Sensor System Based on a Fluorescent Azoalkane

Bakirci

Nau

2005

Adv Funct Materials

127

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The fluorescent azoalkane, 2,3‐diazabicyclo[2.2.2]oct‐2‐ene (DBO), forms inclusion complexes with p‐sulfonatocalix[4]arene (CX4). The binding constants are on the order of 103 M–1 in water. The addition of CX4 to DBO solutions results in an efficient fluorescence quenching (up to 90 %). This supramolecular system can be used as a truly water‐soluble sensor system to signal the binding of organic ammonium ions over a large pH range. Addition of choline and carnitine derivatives and tetraalkylammonium ions results in regeneration of this fluorescence, from which the binding constants (KC = 103–105 M–1) are calculated by means of a competitive complexation model. Electrostatic effects are observed, namely, a more‐than‐one order of magnitude weaker binding of the carnitines in neutral solution.

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“…Recently, liquid crystal (LC)-based sensors have received a huge amount of attention because of their distinctive properties compared with conventional analytical methods, i.e., gas or high-performance liquid chromatography [12,13] and fluorescence labeling [14], which demand time-consuming sample preparation, long separation time, high quantity of sample, and experimental skill [7]. Owing to the various properties of thermotropic liquid crystalline molecules, such as long-range orientational order [15], thermotropic phase transitions, and orientational sensitivity to surface topography and functionality where they contact with [15,16], LC-based sensors provide a novel, label-free detection strategy that enables an analysis of chemical and biological interactions through amplification of the nanoscopic interactions into a microscopic optical signal that is observable by the naked eye using a simple instrument [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

Han

Jang

2015

Colloid Polym Sci

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In this study, we report on a label-free biosensor for the detection of acetylcholine (ACh) and an acetylcholinesterase (AChE) inhibitor, utilizing liquid crystals (LCs) on a polymeric surface with periodic nanostructures. The polymeric nanostructures, which hold sinusoidal anisotropic patterns, were fabricated by a sequential process of poly(dimethylsiloxane) buckling and replication of these patterns on a poly(urethane acrylate) surface where a film of gold was deposited. AChE was then covalently immobilized onto the gold surface. Optical images of nematic 4-cyano-4′-pentylbiphenyl (5CB) revealed that it aligned parallel to the plane of the enzymedecorated surface. However, AChE-mediated hydrolysis of ACh resulted in a plume of choline, acetate, and unreacted ACh, which in turn produced a distinctive optical transition of 5CB (from uniform to random) by masking the anisotropic surface topography. The hydrolysis of ACh was inhibited in the presence of a carbamate insecticide (AChE inhibitor), which prevented the orientational transition of 5CB by decreasing the enzymatic activity of AChE. These results suggest that this LC-based enzymatic sensor is highly sensitive to ACh and the AChE inhibitor, with a detection limit of 10 and 1 nM, respectively. This study demonstrates that polymeric surfaces with continuous wavy features can be used as LCbased biosensors to amplify the existence of ACh and an AChE inhibitor without labeling or an additional amplification strategy.

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Cited by 37 publications

References 20 publications

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Fluorescence Regeneration as a Signaling Principle for Choline and Carnitine Binding: A Refined Supramolecular Sensor System Based on a Fluorescent Azoalkane

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

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