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Vysotskaya, O. V.; Опарина, Л. А.; Паршина, Л. Н.; Гусарова, Н. К.; Трофимов, Б. А.

doi:10.1023/a:1020924804169

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…2-(vinyloxy)ethyl methacrylate was synthesized after a modified procedure of Vysotskaya et al 41 We prepared a simulated body fluid (SBF) following the recipe proposed by Kokubo Dulbecco 0 s phosphate buffered saline (DPBS) and CaCl 2 Á4H 2 O were purchased from Life Technologies and Merck.…”

Section: Methodsmentioning

confidence: 99%

“…All reagents and solvents were of analytical grade and used as received. 2-(vinyloxy)ethyl methacrylate was synthesized after a modified procedure of Vysotskaya et al 41 We prepared a simulated body fluid (SBF) following the recipe proposed by Kokubo and Takadama. 42 The ion concentrations of SBF are as follows: 142.0 mM Na + , 5.0 mM K + , 1.5 mM Mg 2+ , 2.5 mM Ca 2+ , 147.…”

Section: Methodsmentioning

confidence: 99%

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Transformation of vaterite nanoparticles to hydroxycarbonate apatite in a hydrogel scaffold: relevance to bone formation

et al. 2015

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Biomimetic materials have been gaining increasing importance for use as bone biomaterials, because they may provide regenerative alternatives for the use of autologous tissues for bone regeneration. We demonstrate a promising alternative for the use of biomimetic materials based on a biodegradable PEG hydrogel loaded with vaterite nanoparticles as mineral storage. Vaterite, the least stable CaCO 3 polymorph, is stable enough to ensure the presence of a potential ion buffer for bone regeneration, but still has sufficient reactivity for the transformation from CaCO 3 to hydroxyapatite (HA). A combination of powder X-ray diffraction (PXRD), electron microscopy, and Fourier-transform infrared (FT-IR) and Raman spectroscopy showed the transformation of vaterite nanoparticles incorporated in a PEG-acetal-DMA hydrogel to hydroxycarbonate apatite (HCA) crystals upon incubation in simulated body fluid at human body temperature within several hours. The transformation in the PEG-acetal-DMA hydrogel scaffold in simulated body fluid or phosphate saline buffer proceeded significantly faster than for free vaterite. The vaterite-loaded hydrogels were free of endotoxin and did not exhibit an inflammatory effect on endothelial cells. These compounds may have prospects for future applications in the treatment of bone defects and bone degenerative diseases. † Electronic supplementary information (ESI) available: Fig. S1, quantitative phase analysis and determination of the crystallite size based on the XRD data after soaking vaterite nanoparticles in SBF at 37 1C for (a) 24 h (b) 48 h and (c) 72 h. See

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transformation of vaterite nanoparticles to hydroxycarbonate apatite in a hydrogel scaffold: relevance to bone formation

et al. 2015

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“…Dichloromethane (p.a., Sigma-Aldrich) was dried according to literature procedures. 2-(Vinyloxy)ethyl methacrylate was synthesized per a modified procedure of Vysotskaya et al Dulbecco’s phosphate buffered saline (PBS, Invitrogen, Carlsbad, CA), glass beads (1 mm diameter, P. Marienfeld GmbH, Lauda-Königshofen, Germany), ethanol (100% undenatured, Merck, Darmstadt, Germany), grass pollen extract ( Phleum pratense ), dust mite allergen ( Dermatophagoides pteronyssinus ), (both supplied from ALK-Abelló, Hamburg, Germany), Centrisart I tubes (Sartorius, Göttingen, Germany), Alexa Fluor 633 C5 maleimide (A20342), Alexa Fluor 633 protein labeling kit (A20170, Invitrogen), Ficoll Paque 1.077 g/mL (PAA Laboratories GmbH, Cölbe, Germany), IMDM (PAA Laboratories GmbH), LysoTracker Green DND-26 (Molecular Probes, Eugene, OR), Hoechst 33342 (Invitrogen), IL-4, TNF-α, and IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany), GM-CSF (Leukine; Immunex Corp., Seattle, WA), prostaglandin E2 (Cayman Chemical, Ann Arbor, MI), fluorescein isothiocyanate (FITC)-conjugated HLA-DR (L243), phycoerythrin (PE)-conjugated CD80 (L307.4), CD83 (HB15e), CD86 (2331[FUN-1]), and mouse IgG isotype controls (all from BD Biosciences, San Jose, CA), antibody-coated paramagnetic MicroBeads (MACS, Miltenyi Biotec), AlexaFluor 647–conjugated CD4 (MT310; Santa Cruz Biotechnology, Santa Cruz, CA), [methyl- 3 H]thymidine ([3H]TdR; ICN, Irvine, CA), PE Annexin V and 7-AAD (BD Biosciences), and cellular antigen stimulation test (CAST-2000 ELISA; Bühlmann Laboratories AG, Schönenbuch, Switzerland) were used as purchased. All other chemicals and solvents were purchased by Acros Organics, Geel, Belgium.…”

Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Biodegradable pH-Sensitive Poly(ethylene glycol) Nanocarriers for Allergen Encapsulation and Controlled Release

et al. 2015

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In the last decades, the number of allergic patients has increased dramatically. Allergen-specific immunotherapy (SIT) is the only available cause-oriented therapy so far. SIT reduces the allergic symptoms, but also exhibits some disadvantages; that is, it is a long-lasting procedure and severe side effects like anaphylactic shock can occur. In this work, we introduce a method to encapsulate allergens into nanoparticles to avoid severe side effects during SIT. Degradable nanocarriers combine the advantage of providing a physical barrier between the encapsulated cargo and the biological environment as well as responding to certain local stimuli (like pH) to release their cargo. This work introduces a facile strategy for the synthesis of acid-labile poly(ethylene glycol) (PEG)-macromonomers that degrade at pH 5 (physiological pH inside the endolysosome) and can be used for nanocarrier synthesis. The difunctional, water-soluble PEG dimethacrylate (PEG-acetal-DMA) macromonomers with cleavable acetal units were analyzed with 1H NMR, SEC, and MALDI-ToF-MS. Both the allergen and the macromonomers were entrapped inside liposomes as templates, which were produced by dual centrifugation (DAC). Radical polymerization of the methacrylate units inside the liposomes generated allergen-loaded PEG nanocarriers. In vitro studies demonstrated that dendritic cells (DCs) internalize the protein-loaded, nontoxic PEG-nanocarriers. Furthermore, we demonstrate by cellular antigen stimulation tests that the nanocarriers effectively shield the allergen cargo from detection by immunoglobulins on the surface of basophilic leucocytes. Uptake of nanocarriers into DCs does not lead to cell maturation; however, the internalized allergen was capable to induce T cell immune responses.

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Functional Acetal Methacrylates. Part 3. Electrophilic Addition of Diols to 2‐(Vinyloxy)ethyl Methacrylate.

et al. 2003

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Untitled

Cited by 5 publications

References 2 publications

Transformation of vaterite nanoparticles to hydroxycarbonate apatite in a hydrogel scaffold: relevance to bone formation

Transformation of vaterite nanoparticles to hydroxycarbonate apatite in a hydrogel scaffold: relevance to bone formation

Biodegradable pH-Sensitive Poly(ethylene glycol) Nanocarriers for Allergen Encapsulation and Controlled Release

Functional Acetal Methacrylates. Part 3. Electrophilic Addition of Diols to 2‐(Vinyloxy)ethyl Methacrylate.

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