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Benkirane, Hasnae; Sabounji, Karima; Chlyah, Averil; Chlyah, Hassan

doi:10.1023/a:1006464208686

Cited by 44 publications

(9 citation statements)

References 27 publications

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“…No callus was induced with 15-20 mm long leaf explants and only up to 3% with 10-15 mm long leaf explants. Small explants also presented higher morphogenetic capacity in Triticum durum (Benkirane et al 2000) and in Triticum aestivum (Delporte et al 2001). Benkirane et al (2000) postulated that larger explants maintain normal tissue interactions, and such interactions may inhibit cell division through maintenance of symplast domains.…”

Section: Discussionmentioning

confidence: 99%

“…Small explants also presented higher morphogenetic capacity in Triticum durum (Benkirane et al 2000) and in Triticum aestivum (Delporte et al 2001). Benkirane et al (2000) postulated that larger explants maintain normal tissue interactions, and such interactions may inhibit cell division through maintenance of symplast domains. Additionally, explants with reduced size showed synthesis of new cell wall components, such as oligosaccharides, that can act as signals to the cell to reenter the mitotic cycle (Tran Thanh Van and Bui 2000).…”

Section: Discussionmentioning

confidence: 99%

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Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Othmani

Bayoudh²,

Drira³

et al. 2009

Plant Cell Tiss Organ Cult

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Plant regeneration through somatic embryogenesis from young leaf explants (5-10 mm long) adjacent to the apex of 5-6 year old offshoots of Tunisian date palm (Phoenix dactylifera L.), cultivar Boufeggous was successfully achieved. Factors affecting embryogenic callus initiation, including plant growth regulators and explant size, were investigated. The highest induction frequencies of embryogenic calli occurred after 6-7 months on MS medium supplemented with 10 mg l -1 2,4-D and 0.3 mg l -1 activated charcoal. The subculture of these calli onto maintenance medium resulted in the formation of proembryos. Fine chopping and partial desiccation (6 and 12 h) of embryogenic calli with proembryos prior to transfer to MS medium supplemented with 1 mg l -1 ABA stimulated the rapid maturation of somatic embryos. Maturated somatic embryo yield per 0.5 g FW of embryogenic callus was 51 embryos with an average maturation time of 55 days. This was increased to 422 with finely chopped callus, and 124 and 306 embryos following 6 and 12 h desiccation treatments, respectively. The average time to maturation for these 3 treatments was 35, 43 and 38 days, respectively. Subsequent substitution of ABA in MS medium with 1 mg l -1 NAA resulted in the germination and conversion of 81% of the somatic embryos into plantlets with normal roots and shoots. The growth of regenerated somatic plants was also monitored in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Othmani

Bayoudh²,

Drira³

et al. 2009

Plant Cell Tiss Organ Cult

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“…For Cavendish bananas (Musa AAA group), to which most varieties grown in China belong, the embryogenic response is usually less than 1% (Schoofs et al, 1999). On the contrary, the embryogenic callus induction frequencies of most other monocotyledonous crops, such as wheat, barley and sorghum, are significantly higher and range between 20% and 100% (Castillo et al, 1998;Benkirane et al, 2000;Elkonin et al, 2000). Using immature male flowers of five cultivars belonging to three genotypes (Musa AAA/AAB/ABB), Escalant and coworkers (1994) obtained 0-7% embryogenic callus induction, even though the explants were incubated in the optimal season.…”

Section: Discussionmentioning

confidence: 99%

Establishment of embryogenic cell suspensions and plant regeneration of the dessert banana ‘Williams’ (MusaAAA group)

Panis

Strosse

et al. 2005

The Journal of Horticultural Science and Biotechnology

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“…Callus segments also were obtained on MS medium supplemented with 0.56 mg·l −1 BAP, 0.93 mg·l −1 1-naphthaleneacetic acid (NAA) and 0.11 mg·l −1 2,4-dichlorophenoxyacetic acid (2,4-D) [14]. In addition, small callus size has been reported to be an important factor in absorption of nutrients and proliferation of callus in wheat [15]. So to improve callus growth and embryogenic callus formation, we divided the callus into small pieces (50 mg) for each subculture.…”

Section: Callus Inductionmentioning

confidence: 99%

Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of <i>Alhagi camelorum</i>

Honari¹,

Askari²,

Khosrowchahli³

2014

AJPS

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In this study, the desirability function approach was used to simultaneously optimize multiple factors. The variables evaluated were combinations of growth regulators, media and organs in affecting the organogenesis of Alhagi. In the first step of this study, various experiments were carried out in order to optimize the callus induction and its growth. The highest rate of callus induction was achieved from the leaf segment on MS medium supplemented with 3 mg·l −1 BAP + 6 mg·l −1 NAA. Moreover, the highest rate of regeneration was recorded on MS medium supplemented with those 4 mg·l −1 BAP using for leaf segments. The optimized condition of roots induction per shoot was observed in the 1/4 MS medium without activated charcoal and supplemented with 0.25 mg·l −1 IAA. The well rooted plantlets were acclimatized and successfully established into soil where 70% plantlets were survived. The data indicate that media, BAP, and NAA levels may be manipulated to increase or decrease quality factors chosen.

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Cited by 44 publications

References 27 publications

Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Establishment of embryogenic cell suspensions and plant regeneration of the dessert banana ‘Williams’ (MusaAAA group)

Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of <i>Alhagi camelorum</i>

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Untitled

Cited by 44 publications

References 27 publications

Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Somatic embryogenesis and plant regeneration in date palm Phœnix dactylifera L., cv. Boufeggous is significantly improved by fine chopping and partial desiccation of embryogenic callus

Establishment of embryogenic cell suspensions and plant regeneration of the dessert banana ‘Williams’ (MusaAAA group)

Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of &lt;i&gt;Alhagi camelorum&lt;/i&gt;

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Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of <i>Alhagi camelorum</i>