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Goldman, Maria Helena S.; Seurinck, Jef; Marins, Mozart; Goldman, Gustavo Henrique; Mariani, Celestina

doi:10.1023/a:1005914918284

Cited by 21 publications

(2 citation statements)

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“…To identify genes specifically or predominantly expressed in tobacco pistils, a stigma/style cDNA library was previously constructed and a differential screening against seedling cDNA probes was performed (Goldman et al, 1992). This procedure has been successful and several cDNA clones have been isolated and characterized (Goldman et al, 1992(Goldman et al, , 1994(Goldman et al, , 1998. The molecular characterization of a novel stigma/style cDNA clone (pMG13) is shown here.…”

Section: Sequence Analysis Of a Stigma/style-predominant Cdna Clonementioning

confidence: 99%

“…The self-compatible Nicotiana tabacum species was chosen as a model system due to the size of its reproductive organs. A stigma/style cDNA library was constructed and differentially screened (Goldman et al, 1992), resulting in the isolation of several cDNA clones (Goldman et al, 1992(Goldman et al, , 1994(Goldman et al, , 1998. The characterization of a stigma/style predominantly expressed gene (NtWBC1) encoding an ABC transporter is described here.…”

Section: Introductionmentioning

confidence: 99%

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NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

Otsu¹

2004

Journal of Experimental Botany

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To identify genes specifically or predominantly expressed in the stigmas/styles and to establish their possible function in the reproductive process of plants, a tobacco stigma/style cDNA library was constructed and differentially screened, resulting in the isolation of several cDNA clones. The molecular characterization of one of these clones is described here. After sequencing the cDNA and the isolated genomic clone, it was determined that the corresponding gene encodes a protein containing an ATP-binding cassette, characteristic of ABC transporters. This gene, designated as NtWBC1 (Nicotiana tabacum ABC transporter of the White-Brown Complex subfamily), encodes a protein that contains the typical structure of the 'half-transporters' of the White subfamily. To establish the spatial expression pattern of the NtWBC1 gene, northern blot and real-time RT-PCR analyses with total RNA from roots, stems, leaves, sepals, petals, stamens, stigmas/styles, ovaries, and seeds were performed. The result revealed a transcript of 2.5 kb present at high levels in stigmas and styles and a smaller transcript (2.3 kb) present at a lower level in stamens. NtWBC1 expression is developmentally regulated in stigmas/styles, with mRNA accumulation increasing toward anthesis. In situ hybridization experiments demonstrated that NtWBC1 is expressed in the stigmatic secretory zone and in anthers, at the stomium region and at the vascular bundle. NtWBC1 is the first ABC transporter gene with specific expression in plant reproductive organs to be identified and its expression pattern suggests important role(s) in the reproductive process.

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Section: Sequence Analysis Of a Stigma/style-predominant Cdna Clonementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

Otsu¹

2004

Journal of Experimental Botany

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Catechol Oxidase

Eicken¹,

Gerdemann²,

Krebs³

2004

Handbook of Metalloproteins

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Catechol oxidases are ubiquitous plant enzymes with a dinuclear type‐3 copper center. In the wound‐responsive mechanism of the plant, they catalyze the oxidation of a broad range of o‐diphenols to the corresponding o‐quinones coupled with the reduction of oxygen to water. The crystal structures of catechol oxidase from sweet potato in the resting Cu(II)–Cu(II) state, the reduced Cu(I)–Cu(I) form, and in complex with the inhibitor phenylthiourea were analyzed. The catalytic copper center in a central four‐helix bundle is located in a hydrophobic pocket close to the surface. Both metal sites are coordinated by three histidine ligands. His109, ligated to the CuA site, is covalently linked to Cys92 by an unusual thioether bond. On the basis of biochemical, spectroscopic, and structural data, a catalytic mechanism is proposed in which one of the oxygen atoms of the diphenolic substrate binds to CuB of the oxygenated enzyme. There are close structural relationships of catechol oxidase to different hemocyanins.

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Enzymatic and Nonenzymatic Degradation of Polyphenols

López‐Nicolás¹,

García‐Carmona

2009

Fruit and Vegetable Phytochemicals

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Cited by 21 publications

References 22 publications

NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

Catechol Oxidase

Enzymatic and Nonenzymatic Degradation of Polyphenols

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