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Paul, Nigel D.; Rasanayagam, Sharima; Moody, Sandra A.; Hatcher, P. E.; Ayres, P. G.

doi:10.1023/a:1009746511767

Cited by 58 publications

(5 citation statements)

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“…The negative effects of UV light on microorganisms has been widely known and investigated in various microbial ecology studies (Newsham et al, 1997; Paul et al, 1997). In our experiments, the detrimental effect of UV light particularly affected non-formulated KΦ1 phages that were completely inactivated after 40 days, while in conditions of constant darkness, phages survived more than 60 days.…”

Section: Discussionmentioning

confidence: 99%

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage KΦ1, Its Survival and Potential in Control of Pepper Bacterial Spot

et al. 2018

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Xanthomonas euvesicatoria phage KΦ1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of KΦ1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage KΦ1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted KΦ1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to KΦ1. Phage KΦ1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4°C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated KΦ1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage KΦ1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.

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Section: Discussionmentioning

confidence: 99%

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage KΦ1, Its Survival and Potential in Control of Pepper Bacterial Spot

et al. 2018

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“…Bacteriophage LDT325 showed no significant titer change after incubation with chloroform for 1 h, indicating strong tolerance to this reagent, which is consistent with the results of previous studies ( Nordeen et al, 1983 ). The detrimental impacts of UV radiation on microbes have been extensively recognized and explored in studies on microbial ecology ( Paul et al, 1997 ). Long-term UV irradiation significantly affected the phage titer, consistent with previous studies ( Gašić et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Biological characteristics of the bacteriophage LDT325 and its potential application against the plant pathogen Pseudomonas syringae

Liu,

Wang,

Huang

et al. 2024

Front. Microbiol.

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Bud blight disease caused by Pseudomonas syringae is a major bacterial disease of tea plants in China. Concerns regarding the emergence of bacterial resistance to conventional copper controls have indicated the need to devise new methods of disease biocontrol. Phage-based biocontrol may be a sustainable approach to combat bacterial pathogens. In this study, a P. syringae phage was isolated from soil samples. Based on morphological characteristics, bacteriophage vB_PsS_LDT325 belongs to the Siphoviridae family; it has an icosahedral head with a diameter of 53 ± 1 nm and nonretractable tails measuring 110 ± 1 nm. The latent period and burst size of the phage were 10 min and 17 plaque-forming units (PFU)/cell, respectively. Furthermore, an analysis of the biological traits showed that the optimal multiplicity of infection (MOI) of the phage was 0.01. When the temperature exceeded 60°C, the phage titer began to decrease. The phage exhibited tolerance to a wide range of pH (3–11) and maintained relatively stable pH tolerance. It showed a high tolerance to chloroform, but was sensitive to ultraviolet (UV) light. The effects of phage LDT325 in treating P. syringae infections in vivo were evaluated using a tea plant. Plants were inoculated with 2 × 107 colony-forming units (CFU)/mL P. syringae using the needle-prick method and air-dried. Subsequently, plants were inoculated with 2 × 107 PFU/mL LDT325 phage. Compared with control plants, the bacterial count was reduced by 1 log10/0.5 g after 4 days in potted tea plants inoculated with the phage. These results underscore the phage as a potential antibacterial agent for controlling P. syringae.

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“…The role of light on various crops was evaluated [ 22 , 23 , 24 , 25 ]. However, fungal pathogens also react to solar radiation, and it depends on the duration of exposure, wavelengths, and irradiance [ 26 ]. Fungi sense light using 11 photoreceptors, which control morphological and physiological responses [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Sunlight induces photomorphogenesis, phototropism and serves for signal orientation [ 28 ]. The ultraviolet B radiation (UV-B) could positively or negatively affect fungal growth and conidia germination [ 26 ]. Fungal responses to light are reflected by conidia production, stress tolerance, pigmentation, virulence, second metabolism, and germination speed [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Different LED Light Wavelengths and Photosynthetic Photon Flux Density Effect on Colletotrichum acutatum Growth

Rasiukevičiūtė

Brazaitytė

Vaštakaitė-Kairienė

et al. 2022

Plants

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The study aimed to evaluate the effect of different photon flux density (PFD) and light-emitting diodes (LED) wavelengths on strawberry Colletotrichum acutatum growth characteristics. The C. acutatum growth characteristics under the blue 450 nm (B), green 530 nm (G), red 660 nm (R), far-red 735 nm (FR), and white 5700 K (W) LEDs at PFD 50, 100 and 200 μmol m−2 s−1 were evaluated. The effect on C. acutatum mycelial growth evaluated by daily measuring until five days after inoculation (DAI). The presence of conidia and size (width and length) evaluated after 5 DAI. The results showed that the highest inhibition of fungus growth was achieved after 1 DAI under B and G at 50 μmol m−2 s−1 PFD. Additionally, after 1–4 DAI under B at 200 μmol m−2 s−1 PFD. The lowest conidia width was under FR at 50 μmol m−2 s−1 PFD and length under FR at 100 μmol m−2 s−1 PFD. Various LED light wavelengths influenced differences in C. acutatum colonies color. In conclusion, different photosynthetic photon flux densities and wavelengths influence C. acutatum growth characteristics. The changes in C. acutatum morphological and phenotypical characteristics could be related to its ability to spread and infect plant tissues. This study’s findings could potentially help to manage C. acutatum by LEDs in controlled environment conditions.

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Cited by 58 publications

References 0 publications

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage KΦ1, Its Survival and Potential in Control of Pepper Bacterial Spot

Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage KΦ1, Its Survival and Potential in Control of Pepper Bacterial Spot

Biological characteristics of the bacteriophage LDT325 and its potential application against the plant pathogen Pseudomonas syringae

Different LED Light Wavelengths and Photosynthetic Photon Flux Density Effect on Colletotrichum acutatum Growth

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