“…Pathogen molecular characterization is a guide to assess and discard cassava genotypes for integrated pest and disease management (Jorge et al, 2001). Although they are not yet in the field, cassava cultivars transformed for waxy starch (Munyikwa et al, 1997) and resistance to the Basta herbicide (Sarria et al, 2000) are being assessed according to the biosafety protocol and may soon be planted commercially.…”
The main factors concerned with cassava breeding are described, such as origin and domestication, botanic classification and relationship with other species, reproduction mode and floral biology, factors that influence flowering, factors that affect hybridization and seed production, genetics and cytogenetics, qualitative trait heredity, selection criteria for yield, hybridization techniques, general and specific breeding objectives, breeding methods, diffusion of new varieties, current state of breeding, main results and impacts, the future of breeding and cultivars recommended in Brazil. The main results obtained at the national and international level in the areas of germplasm and cassava genetic improvement are presented. A list is included with the description of the cultivars for the Northern, Northeastern, Southeastern, Central-Western and Southern regions of Brazil.
“…Pathogen molecular characterization is a guide to assess and discard cassava genotypes for integrated pest and disease management (Jorge et al, 2001). Although they are not yet in the field, cassava cultivars transformed for waxy starch (Munyikwa et al, 1997) and resistance to the Basta herbicide (Sarria et al, 2000) are being assessed according to the biosafety protocol and may soon be planted commercially.…”
The main factors concerned with cassava breeding are described, such as origin and domestication, botanic classification and relationship with other species, reproduction mode and floral biology, factors that influence flowering, factors that affect hybridization and seed production, genetics and cytogenetics, qualitative trait heredity, selection criteria for yield, hybridization techniques, general and specific breeding objectives, breeding methods, diffusion of new varieties, current state of breeding, main results and impacts, the future of breeding and cultivars recommended in Brazil. The main results obtained at the national and international level in the areas of germplasm and cassava genetic improvement are presented. A list is included with the description of the cultivars for the Northern, Northeastern, Southeastern, Central-Western and Southern regions of Brazil.
“…Though there are attempts to explore the starch biosynthesis in cassava using various techniques ( e.g. , [8,19,20]), the most likely first metabolic pathways of cassava were credited to [6], whose pathways were inferred from the comparative genomic analysis of the full-length cDNA library and established before the release of the cassava genome data. Considering the starch biosynthesis pathway exemplified in their studies, it obviously showed an information deficiency containing several gaps in the resulting pathway.…”
BackgroundCassava is a well-known starchy root crop utilized for food, feed and biofuel production. However, the comprehension underlying the process of starch production in cassava is not yet available.ResultsIn this work, we exploited the recently released genome information and utilized the post-genomic approaches to reconstruct the metabolic pathway of starch biosynthesis in cassava using multiple plant templates. The quality of pathway reconstruction was assured by the employed parsimonious reconstruction framework and the collective validation steps. Our reconstructed pathway is presented in the form of an informative map, which describes all important information of the pathway, and an interactive map, which facilitates the integration of omics data into the metabolic pathway. Additionally, to demonstrate the advantage of the reconstructed pathways beyond just the schematic presentation, the pathway could be used for incorporating the gene expression data obtained from various developmental stages of cassava roots. Our results exhibited the distinct activities of the starch biosynthesis pathway in different stages of root development at the transcriptional level whereby the activity of the pathway is higher toward the development of mature storage roots.ConclusionsTo expand its applications, the interactive map of the reconstructed starch biosynthesis pathway is available for download at the SBI group’s website (http://sbi.pdti.kmutt.ac.th/?page_id=33). This work is considered a big step in the quantitative modeling pipeline aiming to investigate the dynamic regulation of starch biosynthesis in cassava roots.
“…The Guangxi Zhuangzu autonomous region in southern China plans to expand cassava-based ethanol production from 139 million L in 2007 to 1.27 billion L in 2010 (Dai et al, 2006;Drapcho et al, 2008). Cassava is also an important source of industrial starch (Munyikwa et al, 1997;Ihemere et al, 2008). However, the short shelf life of the roots (only 2-3 d) limits cassava's economic and industrial potential.…”
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanideinduced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration.
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