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Yanaihara, Atsushi; Otsuka, Yukiko; Iwasaki, Shinji; Koide, Kazunori; Aida, Tadateru; Okai, Takashi

doi:10.1186/1477-7827-2-66

Cited by 36 publications

(13 citation statements)

References 44 publications

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“…(2) Tissues were stained with 1% toluidine blue instead of hematoxylin. [ 19 ] As toluidine blue is a quick biological stain for nuclei, we could greatly reduce the time of exposure to the environment and minimize the damage to ion channels expressed in the membrane and cytoplasm. (3) Sections were dehydrated in alcohol diluted with RNase-free water and then incubated further,[ 20 ] so that the laser-microdissected endometrium could be detached from the coated glass slides rapidly.…”

Section: Discussionmentioning

confidence: 99%

Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

Shi

Jin

Leng

et al. 2016

Chinese Medical Journal

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Background:Adenomyosis (AM) has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.Methods:Uterine tissue samples from 22 patients (cases) with histologically confirmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K+ channel (BKCa)-α/β subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student's t-test was used to compare the expression.Results:The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease, P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05) decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.Conclusions:The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.

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Section: Discussionmentioning

confidence: 99%

Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

Shi

Jin

Leng

et al. 2016

Chinese Medical Journal

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“…Remodeling of the extracellular matrix to facilitate trophoblast invasion requires a delicate balance between tissue degradation and restoration, mediated by CG-regulated MMPs [19, 23–25] and LIF [26–28]. The expression of LIF is mediated upstream by prokinectin which is also regulated by hCG [29].…”

Section: Biological Actionsmentioning

confidence: 99%

Extragonadal actions of chorionic gonadotropin

Banerjee

Fazleabas

2011

Rev Endocr Metab Disord

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The primary embryonic signal in primates is chorionic gonadotropin (CG, designated hCG in humans), that is classically associated with corpus luteum rescue and progesterone production. However, research over the past decade has revealed the presence of the hCG receptor in a variety of extragonadal tissues. Additionally, discoveries of the multiple variants of hCG, namely, native hCG, hyperglycosylated hCG (hyp-hCG) and the β-subunit of the hyperglycosylated hCG (hCG-free β) has established a role for extragonadal actions of hCG. For the initiation and maintenance of pregnancy, hCG mediates multiple placental, uterine and fetal functions. Some of these include development of syncytiotrophoblast cells, mitotic growth and differentiation of the endometrium, localized suppression of the maternal immune system, modulation of uterine morphology and gene expression and coordination of intricate signal transduction between the endometrium. Recurrent pregnancy loss, preeclampsia and endometriosis are associated with altered responses of hCG, all of which have a detrimental effect on pregnancy. A role for hyp-hCG in mediating the development of both trophoblastic and non-trophoblastic tumors has also been suggested. Other significant non-gonadal applications of hCG include predicting preeclampsia, determining the risk of Down’s syndrome and gestational trophoblastic disease, along with relaxing myometrial contractility and preventing recurrent miscarriages. Presence of hCG free-β in serum of cancer patients enables its usage as a diagnostic tumor marker. Thus, the extragonadal functions of hCG encompasses a wide spectrum of applications and is an open area for continued investigation.

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“…MMP-7, a metalloproteinase shown to be present in the glandular epithelium and known to degrade ECM proteins like proteoglycans, fibronectin, elastin, and casein, has been shown to be suppressed by progesterone in humans thus promoting preservation and cellular differentiation of the endometrium (Osteen et al , 2003). On the other hand, CG up-regulates the expression of MMP-7 in both baboons and humans (Rodgers et al , 1994; Sherwin et al , 2007; Yanaihara et al , 2004), thus being able to overcome the inhibitory effect of progesterone that is seen during the menstrual cycle.…”

Section: Endometrial Responsesmentioning

confidence: 99%

Endometrial responses to embryonic signals in the primate

Banerjee¹,

Fazleabas²

2010

Int. J. Dev. Biol.

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The delicate interaction between an embryo and the uterus to initiate implantation and maintain pregnancy is one of the most elegant and fascinating interactions in human biology. Understanding the molecular events of embryo-maternal interaction is of interest to reproductive biologists, clinicians and couples affected by infertility. We have established the baboon as the non-human primate model for studying embryo implantation. Infusion of chorionic gonadotropin (CG), the major embryonic signal of primates, into the uterine cavity of normal cycling baboons during the window of receptivity induces a myriad of morphological, biochemical and molecular changes in the estrogen and progesterone primed endometrium. The luminal epithelium responds by forming plaques, the overall secretory function of the glandular epithelium increases and the stromal response is characterized by induction of α-smooth muscle actin (αSMA). Cross talk between ovarian and embryonic hormones is evidenced by the fact that these responses are inhibited upon treatment with a progesterone receptor antagonist. CG signals principally through the seven transmembrane LH/CG G-protein coupled receptor, and activates a mitogen activated protein kinase pathway in the endometrial epithelium that is unique and independent of all the classical signaling pathways. In the stromal compartment, CG both rescues stromal fibroblasts from their apoptotic demise and also differentiates them into the decidualized phenotype. We propose that stromal cell survival and differentiation is mediated by a critical modulator of cell fate, Notch-1. Thus, CG is an important embryonic signal which modulates communication between the embryo and the endometrium and induces changes that are critical to successful implantation.

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Abstract: Some of the genes we identified with differential expression are related to the immune system. These results are telling us the new information for understanding the secretory human endometrium.

Cited by 36 publications

References 44 publications

Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

Extragonadal actions of chorionic gonadotropin

Endometrial responses to embryonic signals in the primate

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