Untitled

Synthesis, DNA-binding, and interferon-inducing properties of isatin and benzoisatin hydrazones

“…Previously, we have synthesized a series of aminoacetylhydrazones of acridone (I -VII) [1], 9-formylacridine (VIII -XIV), and 9-formylanthracene (XV -XXI) [2]. The interferon-inducing properties [3] and the ability of inhibiting the polymerase chain reaction (PCR) [1,2] make this group of compounds a promising basis in the search for potential antiviral drugs.…”

“…The interferon-inducing properties [3] and the ability of inhibiting the polymerase chain reaction (PCR) [1,2] make this group of compounds a promising basis in the search for potential antiviral drugs. Indeed, Logan et al [4] demonstrated antiviral activity of isatin derivatives (in particular, compound XXII); Potopal'skii et al [5] reported that 1-methylisatin thiosemicarbazone (compound XXIII, methisazone, isatisone) exhibited high activity against smallpox virus [5].…”

“…Isatin aminoacetylhydrazones (XXXI -XXXVII), as well as their benzoisatin analogs (XXIV -XXX), were synthesized via condensation of the initial compounds XXII and XL with the corresponding aminoacetic acid hydrazides synthesized previously [1].…”

“…The DNA-binding properties of the synthesized compounds were studied by method of EtBr displacement from its complex with calf thymus DNA as described in [1]. According to this method, the intercalation of a given compound is characterized by a decrease in the fluorescence from intercalated EtBr ligand (L) as a result of its competitive displacement from the L -DNA complex.…”

“…This concentration is determined as the abscissa of the bending point on the I (%) versus lg C 50 , where I is the relative fluorescence intensity (percentage of that in the control) at constant EtBr and DNA concentrations [1].…”