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Strausberger, Bill M.; Ashley, M.V.

doi:10.1023/a:1012526315617

Cited by 59 publications

(24 citation statements)

References 18 publications

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“…Where direct comparisons have been made, parasitism frequencies are higher in such habitats than in adjacent grasslands (Hahn and Hatfield 1995, Strausberger and Ashley 1997. That habitat preference is most likely attributable to higher densities of hosts in more-structured habitats and the presence of elevated perches from which cowbirds can search for host nests.…”

Section: Factors Affecting Cowbird Distribution and Abundance At The mentioning

confidence: 99%

Cowbird (Molothrus spp.) Ecology: A Review of Factors Influencing Distribution and Abundance of Cowbirds across Spatial Scales

Chace¹,

Farmer²,

Winfree³

et al. 2005

Ornithological Monographs

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Section: Factors Affecting Cowbird Distribution and Abundance At The mentioning

confidence: 99%

Cowbird (Molothrus spp.) Ecology: A Review of Factors Influencing Distribution and Abundance of Cowbirds across Spatial Scales

Chace¹,

Farmer²,

Winfree³

et al. 2005

Ornithological Monographs

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“…In precocial species where the chicks leave the nesting area as soon as they can walk, eggshell remains can usually be collected after hatch. Egg-shells might be contaminated with parental and sibling DNA (Schmaltz et al 2006;Strausberger and Ashley 2001;Taberlet and Fumagalli 1996;Taberlet and Waits 1998). However, if eggshells would still yield high quality DNA, the collection of eggshells would ease the collection of material for genetic population structure.…”

Section: Introductionmentioning

confidence: 99%

“…For the purpose of maternity and sex determination analyses, several studies have successfully isolated DNA from eggshell membranes (Bush et al 2005;Pearce et al 1997;Strausberger and Ashley 2001). These studies used eggshell membranes or swabs of eggshells as a DNA source, thereby examining 1-4 microsatellite loci only (Bush et al 2005;Pearce et al 1997;Strausberger and Ashley 2001).…”

Section: Introductionmentioning

confidence: 99%

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Using eggshell membranes as a DNA source for population genetic research

et al. 2009

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In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA was not affected (F ST = -0.01735, P = 0.999) by degeneration or possible crosscontamination. Furthermore, neither degeneration nor cross-contamination was apparent in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell membranes can be used for population genetic research.

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“…Strausberger indicated that a single egg could provide both maternal and offspring DNA. The genotypes for three or four loci from the embryos and shell samples were also obtained 10 . Arnold sampled the blastoderm/disc, VM, and a mixture of both.…”

Section: Introductionmentioning

confidence: 99%

The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane

et al. 2017

Sci Rep

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The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells’ viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.

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Cited by 59 publications

References 18 publications

Cowbird (Molothrus spp.) Ecology: A Review of Factors Influencing Distribution and Abundance of Cowbirds across Spatial Scales

Cowbird (Molothrus spp.) Ecology: A Review of Factors Influencing Distribution and Abundance of Cowbirds across Spatial Scales

Using eggshell membranes as a DNA source for population genetic research

The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane

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