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Momotaz, Aliya; Kato, Masahiro; Kakihara, Fumika

doi:10.1023/a:1018331528368

Cited by 46 publications

(12 citation statements)

References 20 publications

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“…Utilization of embryo rescue or somatic hybridization techniques, or combination of both, could help to overcome natural reproductive barriers in the development of inter-specific hybrids in Brassica (Ayotte et al, 1987; Hansen and Earle, 1995; Momotaz et al, 1998; Weerakoon et al, 2009; Niemann et al, 2013). Wide crosses between crop plants and their wild relatives have now become routinely possible through the use of the embryo rescue technique.…”

Section: Introductionmentioning

confidence: 99%

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

et al. 2017

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Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is a very important disease of cauliflower (Brassica oleracea botrytis group) resulting into 10–50% yield losses every year. Since there is a dearth of availability of resistance to black rot disease in B. oleracea (C genome), therefore exploration of A and B genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. To utilize these sources, interspecific hybrid and backcross progeny (B1) were generated between cauliflower “Pusa Sharad” and Ethiopian mustard “NPC-9” employing in vitro embryo rescue technique. Direct ovule culture method was better than siliqua culture under different temperature regime periods. Hybridity testing of F1 inter-specific plants was carried out using co-dominant SSR marker and Brassica B and C genome-specific (DB and DC) primers. Meiosis in the di-genomic (BCC) interspecific hybrid of B. oleracea botrytis group (2n = 18, CC) × B. carinata (2n = 4x = 34, BBCC) was higly disorganized and cytological analysis of pollen mother cells revealed chromosomes 2n = 26 at metaphase-I. Fertile giant pollen grain formation was observed frequently in interspecific F1 hybrid and BC1 plants. The F1 inter-specific plants were found to be resistant to Xcc race 1. Segregation distortion was observed in BC1 generation for black rot resistance and different morphological traits. The At1g70610 marker analysis confirmed successful introgression of black rot resistance in interspecific BC1 population. This effort will go a long way in pyramiding gene(s) for resistance against black rot in Cole crops, especially cauliflower and cabbage for developing durable resistance, thus minimize dependency on bactericides.

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Section: Introductionmentioning

confidence: 99%

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

et al. 2017

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“…Natural crossing among the Brassica species was reported to occur with varying difficulty (Myers 2006). However, the majority of hybrid production from crosses between a diploid and tetraploid Brassica species has been successful when in vitro techniques such as embryo rescue or protoplast fusions were employed (Bing et al 1995; Momotaz et al 1998; Hu et al 2002). In this study, we report for the first time the successful production of hybrids and transfer of auxinic herbicide resistance from B. kaber to B. juncea × B. kaber hybrids.…”

Section: Resultsmentioning

confidence: 99%

“…Nonetheless, trait transfer among Brassica members is not always straightforward, due to variations in chromosome number as well as ploidy among species (Mizushima 1950, 1980). Therefore, in vitro techniques such as ovule/embryo rescue is commonly employed to successfully produce interspecific hybrids among Brassica members (Inomata 1988; Mathias 1991; Bing et al 1995; Momotaz et al 1998; Tonguc and Griffiths 2004). …”

Section: Introductionmentioning

confidence: 99%

Transfer of auxinic herbicide resistance from Brassica kaber to Brassica juncea and Brassica rapa through embryo rescue

Jugulam

Hall

2013

In Vitro Cell.Dev.Biol.-Plant

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Auxinic herbicides are widely used in agriculture to selectively control broadleaf weeds. Prolonged use of auxinic herbicides has resulted in the evolution of resistance to these herbicides in some biotypes of Brassica kaber (wild mustard), a common weed in agricultural crops. In this study, auxinic herbicide resistance from B. kaber was transferred to Brassica juncea and Brassica rapa, two commercially important Brassica crops, by traditional breeding coupled with in vitro embryo rescue. A high frequency of embryo regeneration and hybrid plant establishment was achieved. Transfer of auxinic herbicide resistance from B. kaber to the hybrids was assessed by whole-plant screening of hybrids with dicamba, a widely used auxinic herbicide. Furthermore, the hybrids were tested for fertility (both pollen and pistil) and their ability to produce backcross progeny. The auxinic herbicide-resistant trait was introgressed into B. juncea by backcross breeding. DNA ploidy of the hybrids as well as of the backcross progeny was estimated by flow cytometry. Creation of auxinic herbicide-resistant Brassica crops by non-transgenic approaches should facilitate effective weed control, encourage less tillage, provide herbicide rotation options, minimize occurrence of herbicide resistance, and increase acceptance of these crops.

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“…Nevertheless, development of interspecific hybrid is challenging due to abortion of hybrid embryos associated with pre-and post-fertilization barriers and also due to the existence of a U-triangle relationship between six Brassica species (Zhang et al 2001). Utilizing embryo rescue protocols is expected to achieve success from the interspecific hybridization by overcoming the associated natural barriers (Momotaz et al 1998;Weerakoo et al 2009;Sharma et al 2017). Distant crosses between crop plants and their wild relatives most often require embryo rescue technique to save developing embryos.…”

Section: Introductionmentioning

confidence: 99%

Prospects of Embryo Rescue in Developing Novel Brassica Genotypes

Ripa

Jyoti

Robin

2023

Plant Breed. Biotech.

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Interspecific hybridization creates new genetic variants. Embryo formation and subsequently fertile seed development are the indicators of successful interspecific hybridization. Often interspecific hybridization is limited due to variations in genome and ploidy levels. The technique of embryo rescue is commonly used in interspecific hybridization to facilitate the survival of embryos from abortion. The effectiveness of an embryo rescue program in the Brassica species depends on embryo age, embryo development stage and media composition. Embryo rescue protocol could be effectively utilized to solve some major problems of the oilseed Brassica growers including blackleg, clubroot and Alternaria disease resistance, salinity, and drought tolerance etc. This review article discusses the prospects of developing novel Brassica hybrid genotypes with desirable traits through embryo rescue.

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Cited by 46 publications

References 20 publications

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

Introgression of Black Rot Resistance from Brassica carinata to Cauliflower (Brassica oleracea botrytis Group) through Embryo Rescue

Transfer of auxinic herbicide resistance from Brassica kaber to Brassica juncea and Brassica rapa through embryo rescue

Prospects of Embryo Rescue in Developing Novel Brassica Genotypes

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