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Doan, Nga Thanh; Rickards, Rodney W.; Rothschild, Jennifer M.; Smith, Geoffrey D.

doi:10.1023/a:1008170007044

Cited by 119 publications

(8 citation statements)

References 20 publications

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“…Standard deviation is given in parentheses. The average values of three inhibition zone diameters and standard deviation were calculated using Excel, standard functions was applied to a silica gel G60 (Merck) column (1.5×25 cm) prepared from a slurry of 30 g of precipitated Silica gel G60 (Doan et al 2000). The column was developed using the solvent system sequence in Table 1.…”

Section: Extraction and Column Chromatography Of Lipophilic Fractionsmentioning

confidence: 99%

“…Despite the fact that several studies have focused on the characterisation of bioactive compounds from different cyanobacterial strains from different geographical locations (Falch et al 1995;Doan et al 2000;Ghasemi et al 2004;Asthana et al 2006), antimicrobial compounds derived from Egyptian cyanobacterial flora have rarely been characterised, except for some limited efforts such as those by Issa (1999), El-Sheekh et al (2006), El Semary et al (2009) and El Semary and Abd El Naby (2010).…”

Section: Introductionmentioning

confidence: 99%

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The characterisation of bioactive compounds from an Egyptian Leptolyngbya sp. strain

Semary

2011

Ann Microbiol

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An investigation into the bioactive metabolites from a benthic, mat-forming strain dominating a polluted wastewater canal in Egypt was conducted. Phytochemical screening revealed the presence of saponins, flavonoids and alkaloids; vitamin C was also found at high concentrations. The isolate was investigated as a source of antimicrobial compounds. The lipophilic fraction was extracted using chloroform/methanol and bioassays for antimicrobial compounds were performed using strains of pathogenic bacteria. The fraction that showed the highest bioactivity was purified and its structure elucidated using UV, FTIR, proton-NMR and GC-MS. The compound's molecular weight was 220 and it was identified as butylated hydroxytoluene which has both antimicrobial and antioxidant activities. On the ecological front, this compound, and the other metabolites detected, seem to enable the isolate to dominate its niche and protect it from adverse conditions. On the commercial front, this compound is used as a food additive and was recently discovered in different cyanobacteria, and can be used as a lead compound for both drug and food industries to substitute for the expensive and hazardous synthetic analogs. Therefore, this strain can be considered as a potential source of bioactive compounds that should be further explored.

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Section: Extraction and Column Chromatography Of Lipophilic Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The characterisation of bioactive compounds from an Egyptian Leptolyngbya sp. strain

Semary

2011

Ann Microbiol

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“…To date, only a very limited number of the cyanobacterial allelochemicals has been identified and mainly from freshwater cyanobacteria (Leflaive and Ten-Hage, 2007; Leão et al, 2009). These include cyanobacterin, a chlorinated γ-lactone produced by Scytonema hofmanni that inhibits other cyanobacteria and green microalgae (Mason et al, 1982); fisherellin A, an eynediyne-containing photosystem II inhibitor produced by Fisherella muscicola (Hagmann and Jüttner, 1996); the hapalindoles, small metabolites that have been isolated from Hapalosiphon (Moore et al, 1984; Thanh Doan et al, 2000) that show inhibitory effects against several microorganisms and the nostocyclamides, relatively small cyclic peptides produced by Nostoc sp. that inhibit cyanobacteria and microalgae (Todorova et al, 1995; Jüttner et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.

2016

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Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analyzed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin.

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“…For the preparation of crude extract 40–45 days old cyanobacterial culture was used based on the method of Doan et al (2000). Cyanobacterial cells (10 g fresh weight) were harvested by centrifugation at 10,000 rpm for 15 min (Remi, India) and then lyophilized (Christ-Alpha 1-2, Germany).…”

Section: Methodsmentioning

confidence: 99%

Structural Elucidation and Molecular Docking of a Novel Antibiotic Compound from Cyanobacterium Nostoc sp. MGL001

et al. 2016

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Cyanobacteria are rich source of array of bioactive compounds. The present study reports a novel antibacterial bioactive compound purified from cyanobacterium Nostoc sp. MGL001 using various chromatographic techniques viz. thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Further characterization was done using electrospray ionization mass spectroscopy (ESIMS) and nuclear magnetic resonance (NMR) and predicted structure of bioactive compound was 9-Ethyliminomethyl-12-(morpholin - 4 - ylmethoxy) -5, 8, 13, 16–tetraaza–hexacene - 2, 3 dicarboxylic acid (EMTAHDCA). Structure of EMTAHDCA clearly indicated that it is a novel compound that was not reported in literature or natural product database. The compound exhibited growth inhibiting effects mainly against the gram negative bacterial strains and produced maximum zone of inhibition at 150 μg/mL concentration. The compound was evaluated through in silico studies for its ability to bind 30S ribosomal fragment (PDB ID: 1YRJ, 1MWL, 1J7T, and 1LC4) and OmpF porin protein (4GCP, 4GCQ, and 4GCS) which are the common targets of various antibiotic drugs. Comparative molecular docking study revealed that EMTAHDCA has strong binding affinity for these selected targets in comparison to a number of most commonly used antibiotics. The ability of EMTAHDCA to bind the active sites on the proteins and 30S ribosomal fragments where the antibiotic drugs generally bind indicated that it is functionally similar to the commercially available drugs.

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Cited by 119 publications

References 20 publications

The characterisation of bioactive compounds from an Egyptian Leptolyngbya sp. strain

The characterisation of bioactive compounds from an Egyptian Leptolyngbya sp. strain

A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.

Structural Elucidation and Molecular Docking of a Novel Antibiotic Compound from Cyanobacterium Nostoc sp. MGL001

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