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Avelizapa, Luz Irene Rojas; Cruz‐Camarillo, Ramón; Guerrero, Milena; Rodrı́guez-Vázquez, Refugio; Ibarra, Jorge E.

doi:10.1023/a:1008947029713

Cited by 79 publications

(16 citation statements)

References 26 publications

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“…Based on the previous in vitro antagonistic assay, a total of 10 Trichoderma strains Trichoderma asperellum CCTCC-SBW0102 (T3), T. aureoviride CCTCC-SBW0005 (T9), T. harzianum CCTCC-SBW0101 (T11), T. asperellum CCTCC-SBW0109 (T12), T. atroviride CCTCC-SBW0016 (T21), T. asperellum CCTCC-SBW0052 (T43), T. asperellum CCTCC-SBW0091 (T44), T. tawa (T64), T. harzianum CCTCC-RW0024 (T72), and T. harzianum CCTCC-SBW0181 (T88) were selected for the preliminary enzyme assay 41–43 . The percentage of the enzyme activity was calculated using the following formula as Hydrolysis capacity (%) = Diameter of clear zone (cm)/Diameter of Trichoderma colony (cm).…”

Section: Methodsmentioning

confidence: 99%

Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Saravanakumar

et al. 2017

Sci Rep

155

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Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although Trichoderma species are known as BCA, the selection of host-pathogen specific Trichoderma is essential for the successful field application. Hence, we screened a total of 100 Trichoderma isolates against FG, selected Trichoderma harzianum (CCTCC-RW0024) for greenhouse experiments and studied its effect on changes of maize rhizosphere microbiome and biocontrol of FSR. The strain CCTCC-RW0024 displayed high antagonistic activity (96.30%), disease reduction (86.66%), biocontrol-related enzyme and gene expression. The root colonization of the strain was confirmed by eGFP tagging and qRT-PCR analysis. Pyrosequencing revealed that exogenous inoculation of the strain in maize rhizosphere increased the plant growth promoting acidobacteria (18.4%), decreased 66% of FG, and also increased the plant growth. In addition, metabolites of this strain could interact with pathogenicity related transcriptional cofactor FgSWi6, thereby contributing to its inhibition. It is concluded that T. harzianum strain CCTCC-RW0024 is a potential BCA against FSR.

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Section: Methodsmentioning

confidence: 99%

Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Saravanakumar

et al. 2017

Sci Rep

155

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“…Chitinase activity was evaluated by measuring the width of the edge of the translucent haloes surrounding the colonies grown on the plates after 48 h of incubation (Rojas-Avelizapa et al, 1999). The medium was prepared with 13-14% (wet weight) colloidal chitin and 2.3% agar dissolved in Castaneda medium (0.625 g diammonium citrate, 0.250 g NaCl, 0.375 g KH 2 PO 4 , 0.125 g MgSO 4 •7H 2 O, 0.375 g Na 2 CO 3 , 6.5 mL glycerol, in 1 L distilled water; pH 6.5-7.0).…”

Section: Pot Experimentsmentioning

confidence: 99%

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Ling

Liu

Cheng

et al. 2017

Span. j. agric. res.

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Phosphate-solubilizing bacteria (PSB) have the ability to solubilize insoluble phosphorus (P) and release soluble P. Extensive research has been performed with respect to PSB isolation from the rhizospheres of various plants, but little is known about the prevalence of PSB in the grapevine rhizosphere. In this study, we aimed to isolate and identify PSB from the grapevine rhizosphere in five vineyards of Northwest China, to characterize their plant-growth-promoting (PGP) traits, evaluate the effect of stress on their phosphate-solubilizing activity (PSA), and test their ability to stimulate the growth of Vitis vinifera L. cv. Cabernet Sauvignon. From the vineyard soils, 66 PSB isolates were screened, and 10 strains with high PSA were identified by 16S rRNA sequencing. Sequence analysis revealed that these 10 strains belonged to 4 genera and 5 species: Bacillus aryabhattai, B. megaterium, Klebsiella variicola, Stenotrophomonas rhizophila, and Enterobacter aerogenes. The selected PSB strains JY17 (B. aryabhattai) and JY22 (B. aryabhattai) were positive for multiple PGP traits, including nitrogen fixation and production of indole acetic acid (IAA), siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinase, and protease. JY17 and JY22 showed strong PSA under stress conditions of high pH, high salt, and high temperature. Therefore, these two isolates can be used as biofertilizers in saline-alkaline soils. The inoculation with PSB significantly facilitated the growth of V. vinifera cv. Cabernet Sauvignon under greenhouse conditions. Use of these PSB as biofertilizers will increase the available P content in soils, minimize P-fertilizer application, reduce environmental pollution, and promote sustainable agriculture.Additional key words: promote plant growth; inorganic phosphate; stress conditions; saline-alkaline soil; Vitis vinifera L. cv. Cabernet SauvignonAbbreviations used: ACC (1-aminocyclopropane-1-carboxylate); CAS (chrome azurol S); DF (Dworkin-Foster); GADH (gluconate dehydrogenase); IAA (indole acetic acid); NBRIP (National Botanical Research Institute's phosphate); PGP (plant-growthpromoting); PQQ-GDH (pyrroloquinoline quinone-dependent glucose dehydrogenase); PSA (phosphate-solubilizing activity); PSB (phosphate-solubilizing bacteria); TCP (tricalcium phosphate).Authors' contributions: ML drafted the manuscript; XL made critical revision of the manuscript for important intellectual content; BSC and XLM acquired, analyzed and interpreted data; XTL coordinated the research project; XFZ performed statistical analysis; YLJ and ZM proposed revision suggestions; YLF supervised the work.Citation: Liu, M.; Liu, X.; Cheng, B. S.; Ma, X. L.; Lyu, X. T.; Zhao, X. F.; Ju, Y. L.; Min, Z.; Fang, Y. L. (2016). Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers.

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“…A standard curve of tyrosine (0-300 lM/ml) was estimated to determine protease units (PU). One PU was defined as the amount of enzyme required to release 1 lg of tyrosine min -1 (Rojas- Avelizapa et al 1999).…”

Section: Proteolytic Activitymentioning

confidence: 99%

Hyperproduction of chitinase influences crystal toxin synthesis and sporulation of Bacillus thuringiensis

Barboza‐Corona

Ortiz-Rodríguez

Fuente-Salcido

et al. 2009

Antonie van Leeuwenhoek

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Bacillus thuringiensis HD-73 was transformed with the endochitinase gene chiA74 under the control of a strong promoter (pcytA) and a 5' mRNA stabilizing (STAB-SD) sequence (HD-73-pEBchiA74). Expression levels were compared with those observed from the wild type strain (HD-73) and the recombinant HD-73 strain expressing chiA74 under the control of its native promoter (HD-73-pEHchiA74). The chitinolytic activity of HD-73-pEBchiA74 was markedly elevated, being ~58- and 362-fold higher than, respectively, HD-73-pEHchiA74 and parental HD-73, representing the highest levels of chitinase expression in recombinant B. thuringiensis reported to date. Parasporal crystals measured under transmission electron microscopy showed that HD-73 produced crystals of 1.235 (+/-0.214) and 1.356 (+/-0.247) mum in length when the bacterium was grown in respectively, NBS and NBS with glucose. Otherwise, HD-73-pEBchiA74 synthesized crystals of 1.250 (+/-0.222) and 1.139 (+/-0.202) mum in length when cultivated in NBS and NBS with glucose, respectively, values that showed a diminution of ~10 and 20% compared with crystals produced by HD-73-pEHchiA74 grown under the same conditions. Comparison of viable spore counts per ml showed that HD-73-pEBchiA74 produced fewest viable spores (1.5 x 10(9), 1.3 x 10(9)), compared to HD-73-pEHchiA74 (4.9 x 10(9), 5.3 x 10(9)) and HD-73 (6.8 x 10(9), 8.8 x 10(9)) when grown in NBS and NBS supplemented with glucose, respectively. No change in cellular protease activity was observed despite the overproduction of the chitinase.

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Untitled

Cited by 79 publications

References 26 publications

Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Hyperproduction of chitinase influences crystal toxin synthesis and sporulation of Bacillus thuringiensis

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