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Galeotti, Marco; Beraldo, Paola; Dominis, S. de; D'Angelo, L.; Ballestrazzi, Rodolfo; Musetti, Rita; Pizzolito, S.; Pinosa, M.

doi:10.1023/a:1007883008076

Cited by 34 publications

(9 citation statements)

References 11 publications

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“…Operculum complex and caudal fin complex were the most influenced structures. In gilthead seabream and many other species, operculum complex deformities are frequent [9,[29][30][31][32]. The frequency of opercular anomalies on Ctrl groups (9.7 and 21.3%) was in the range of 6.3 to 43.2% as registered in previous studies conducted over the past two decades [9,30].…”

Section: Discussionmentioning

confidence: 63%

The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream (Sparus aurata)

Mhalhel

Germanà

Abbate

et al. 2020

IJMS

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The gilthead seabream larval rearing in continuous light is common in most Mediterranean hatcheries to stimulate larval length growth and increase food consumption. Several studies have shown that continuous light affects larval development and increases the prevalence of skeletal deformities. Melatonin is a crucial pineal neurohormone that displays daily secretion patterns, stimulates cell proliferation and embryonic development in Atlantic salmon and zebrafish, and improves osseointegration in mice and humans. However, no studies have examined the effects of orally supplemented melatonin on skeletal deformities in Sparus aurata larvae. We administered exogenous melatonin to gilthead seabream larvae via enriched rotifers and nauplii of Artemia. Exogenous melatonin induced bone deformities and stimulated parathyroid hormone-related protein-coding gene (PTHrP) mRNA expression. In addition to the melatonin-induced PTHrP high expression level, the recorded non coordinated function of skeletal muscle and bone during growth can be the fountainhead of bone deformities. Both myosin light chain 2 (mlc2) and bone gamma-carboxyglutamate protein-coding gene (bglap) expression levels were significantly affected by melatonin administration in an inverse dose–response manner during the exogenous melatonin administration. This is the first study to report the effect of inducing melatonin bone deformities on Sparus aurata larvae reared under ordinary hatchery conditions.

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Section: Discussionmentioning

confidence: 63%

The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream (Sparus aurata)

Mhalhel

Germanà

Abbate

et al. 2020

IJMS

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“…As a case study, we explored differences in BMD levels in normal versus deformed opercular bones in gilthead sea bream Sparus aurata (Linnaeus, 1758), and compared those with mineralization patterns in other cranial elements (premaxilla, first vertebra, otolith). Gilthead sea bream is one of the most intensively cultivated fish species in the Mediterranean, but is also the subject of numerous studies that focus on skeletal anomalies (PaPerna 1978;Francescon et al 1988;anDraDes et al 1996;KoumounDouros et al 1997a;galeotti et al 2000;Boglione et al 2001;FernánDeZ et al 2008;Prestinicola et al 2014). Among these skeletal anomalies, opercular deformities are frequently found both during early life stages [in larvae from 17 days post hatching (DPH)] and in adults of farmed gilthead sea bream (KoumounDouros et al 1997b;galeotti et al 2000;BeralDo et al 2003;ortiZ-DelgaDo et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Gilthead sea bream is one of the most intensively cultivated fish species in the Mediterranean, but is also the subject of numerous studies that focus on skeletal anomalies (PaPerna 1978;Francescon et al 1988;anDraDes et al 1996;KoumounDouros et al 1997a;galeotti et al 2000;Boglione et al 2001;FernánDeZ et al 2008;Prestinicola et al 2014). Among these skeletal anomalies, opercular deformities are frequently found both during early life stages [in larvae from 17 days post hatching (DPH)] and in adults of farmed gilthead sea bream (KoumounDouros et al 1997b;galeotti et al 2000;BeralDo et al 2003;ortiZ-DelgaDo et al 2014). This type of skeletal deformity can significantly negatively affect their growth rate and commercial value at market size (Boglione et al 2001;FernánDeZ et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A 3D quantitative method for analyzing bone mineral densities: a case study on skeletal deformities in the gilthead sea bream, Sparus aurata (Linnaeus, 1758)

et al. 2018

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Skeletal deformities, one of the major threats for aquaculture, have been studied extensively. These include opercular malformations in gilthead sea bream (Sparus aurata), a key fish species for Mediterranean aquaculture. What is causing it and at what morphogenetic level it arises, however, is still unclear. Here we focus on bone formation, at the level of bone mineralization. Several methods have been used to study bone mineralization density (BMD), however, these are frequently limited when targeting a high-resolution, three-dimensional mapping of BMD. We used micro-computed tomography (micro-CT) data to perform such a 3D quantification of BMD levels in gilthead sea bream that showed different levels of opercular bone deformations. This approach has the advantage of not having to rely on calibration phantoms, as long as relative BMD values are needed. The results show an increased BMD in deformed opercles compared to normal ones, especially in a bilaterally-deformed specimen. Furthermore, we show that opercular deformations are not necessarily associated with similar mineralization patterns in other mineralized cranial elements, except for the otoliths. Also, mineralization seems to occur left-right independently, matching earlier observations of such an independency of the opercular phenotype as a whole. This study confirms that a quantitative characterization of BMD patterns in 3D is feasible, even in smaller specimens, and that it has several advantages over other commonly used approaches.

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“…abnormalities, opercular deformities represent a common morphological abnormality and are frequently found during the rearing period (Beraldo, Pinosa, Tibaldi, & Canavese, 2003;Galeotti et al, 2000;Koumoundouros, Oran, Divanach, Stefanakis, & Kentouri, 1997). Opercular deformations in sea bream fingerlings have been shown to be heritable (Navarro et al, 2009;Negrin-Baez et al, 2015), associated with inbreeding of broodstocks (Astorga et al, 2003), and several nutritional causes have been suggested, like vitamins and fatty acids (Gapasin, Bombeo, Lavens, Sorgeloos, & Nelis, 1998;Gapasin & Duray, 2001;Koumoundouros, 2010).…”

mentioning

confidence: 99%

“…In S. aurata, abnormal opercular bones, frequently curving inward into and/or outward from the gill cavity, can be observed already at early larval stages (17-19 days post-hatching-dph; Galeotti et al, 2000;Thuong et al, 2017). Such a folding of the opercular bone and even complete lack of it was observed both uni-and bilaterally (Galeotti et al, 2000), with a contralateral independence of the opercular phenotype (Verhaegen, Adriaens, Wolf, Dhert, & Sorgeloos, 2007).…”

mentioning

confidence: 99%

Morphological and histological characterization of an ectopically mineralized structure in a gilthead sea bream Sparus aurata with opercular deformation

Thuong

Prondvai

Kegel

et al. 2019

Journal of Fish Diseases

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In this study, we describe an abnormal ectopically mineralized structure (EMS) that was found inside the skull of a juvenile Sparus aurata that also showed a bilateral opercular deformation. The overall phenotype and tissue composition were studied using micro‐CT scanning and histological analyses. The ectopic structure occupies a large volume of the brain cavity, partially extruding into the gill cavity. It shows a dense mineralization and an extracellular matrix‐rich phenotype, with variation in both the morphology and size of the cell lacunae, combined with an irregular fibre organization inside the matrix. This study is the first to report such an EMS in a juvenile teleost fish, where the tissue does not resemble any other connective tissue type described in bony fish so far. The tissue phenotype seems to rule out that the EMS corresponds to a tumorous cartilage. Yet, it is rather reminiscent of a highly mineralized structure found in cartilaginous fish, where it is suggested to be associated with damage repair.

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Cited by 34 publications

References 11 publications

The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream (Sparus aurata)

The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream (Sparus aurata)

A 3D quantitative method for analyzing bone mineral densities: a case study on skeletal deformities in the gilthead sea bream, Sparus aurata (Linnaeus, 1758)

Morphological and histological characterization of an ectopically mineralized structure in a gilthead sea bream Sparus aurata with opercular deformation

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