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Fernandez, Diana; Ouinten, Mohamed; Tantaoui, A.; Geiger, Jean-Paul

doi:10.1023/a:1008644515046

Cited by 18 publications

(6 citation statements)

References 16 publications

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“…The presence of many formae speciales of F. oxysporum can be detected by PCR [20,21]. Specific oligonucleotides have been developed for rapid identification of pathogens with PCR assay [15]. PCR base identification confirmed that the isolates were F. oxysporum var.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenic and molecular detection of Fusarium oxysporum f. sp. albedinis isolates from different areas in southwest Algeria

Benlarbi¹,

Bellahcene²,

Mebarki³

et al. 2018

Trop. J. Pharm Res

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Purpose: To investigate the intra-specific variations in eleven Fusarium oxysporum isolates from infected date palm using pathogenicity and molecular methods. Methods: Eleven isolates of Fusarium oxysporum obtained from infected date palms in the southwest region of Algeria were subjected to confirmatory test using a specific polymerase chain reaction (PCR) technique with the primer pairs, TL3-FOA28 and BIO3-FOA1. Polymorphism in the 5' domain of the large subunit rRNA was investigated. Small libraries of the domain, amplified by the primer pair, LR3/LROR, were constructed and the inserts sequenced. Results: The 11 isolates of Fusarium oxysporum collected from the infected date palm were confirmed as Fusarium oxysporun f. sp albedinis. Results from the investigation of polymorphism in the 5' domain of the large subunit rRNA revealed that the sequences were 100 % homologous or extremely close (> 99.4 %, differing by no more than one to three nucleotides) to several Fusarium oxysporum sequences. In addition, F. inflexum (U34548.1) was highly homologous to one of the F. oxysporum f. sp. albedinis. Conclusion: The sequences of the 11 isolates are almost 100 % homologous to several F. oxysporum species. It is noteworthy that a sequence highly homologous to one of the F. oxysporum f. sp. albedinis is obtainable from a different species, F. inflexum (U34548.1).

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Section: Discussionmentioning

confidence: 99%

“…The DNA samples were also amplified using the Foa specific primer pairs (TL3-FOA28) and (BIO3-FOA1) [15]. These primer pairs give products of 400 bp and 204 bp, respectively.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic and molecular detection of Fusarium oxysporum f. sp. albedinis isolates from different areas in southwest Algeria

Benlarbi¹,

Bellahcene²,

Mebarki³

et al. 2018

Trop. J. Pharm Res

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“…These regions are the primary centres of date palm cultivation worldwide. However, the crop faces significant challenges from a wide range of insect pests and pathogens, resulting in major economic losses ( Fernandez et al, 1997 ; Sedra, 2003 ; Bouhlali et al, 2020 ). The western genetic pool in North Africa is particularly vulnerable to the presence of Bayoud disease, a devastating vascular wilt.…”

Section: Introductionmentioning

confidence: 99%

“…The wilt occurs as a result of root colonization followed by the host vascular system leading to the death of date palms. According to several studies ( Louvet and Toutain, 1981 ; Fernandez et al, 1997 , 1998 ), Bayoud disease has likely originated in Morocco from which it spread to Algeria and Mauritania and now represents an epiphytotic disease which is difficult to control. Moreover, FOA has high incidence across high-quality date palm varieties (e.g., Majhoul, Boufeggous, Deglat Nour) as they are highly susceptible to the infection.…”

Section: Introductionmentioning

confidence: 99%

Chromosome-scale assembly uncovers genomic compartmentation of Fusarium oxysporum f. sp. albedinis, the causal agent of Bayoud disease in date palm

Khayi,

Armitage,

Gaboun

et al. 2023

Front. Microbiol.

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Date palm (Phoenixdactylifera) is the most significant crop across North Africa and the Middle East. However, the crop faces a severe threat from Bayoud disease caused by the fungal pathogen Fusarium oxysporum f. sp. albedinis (FOA). FOA is a soil-borne fungus that infects the roots and vascular system of date palms, leading to widespread destruction of date palm plantations in North Africa over the last century. This is considered the most devastating pathogen of oasis agriculture in North Africa and responsible for loss of 13 million trees in Algeria and Morocco alone. In this study, we present a chromosome-scale high-quality genome assembly of the virulent isolate Foa 44, which provides valuable insights into understanding the genetic basis of Bayoud disease. The genome assembly consists of 11 chromosomes and 40 unplaced contigs, totalling 65,971,825 base pairs in size. It exhibits a GC ratio of 47.77% and a TE (transposable element) content of 17.30%. Through prediction and annotation, we identified 20,416 protein-coding genes. By combining gene and repeat densities analysis with alignment to Fusarium oxysporum f. sp. lycopersici (FOL) 4287 isolate genome sequence, we determined the core and lineage-specific compartments in Foa 44, shedding light on the genome structure of this pathogen. Furthermore, a phylogenomic analysis based on the 3,292 BUSCOs core genome revealed a distinct clade of FOA isolates within the Fusarium oxysporum species complex (FOSC). Notably, the genealogies of the five identified Secreted In Xylem (SIX) genes (1, 6, 9, 11 and 14) in FOA displayed a polyphyletic pattern, suggesting a horizontal inheritance of these effectors. These findings provide a valuable genomics toolbox for further research aimed at combatting the serious biotic constraints posed by FOA to date palm. This will pave the way for a deeper understanding of Bayoud disease and facilitate the development of effective diagnostic tools and control measures.

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“…Foa is a soil-borne parasite that infects plant vessels and produces chlamydospores, which can survive in plant vessels and deeply in the soil [1]. Indeed, the studies carried out on the genetic diversity of Foa isolates from different stations have shown that they have a single clonal origin [4][5][6][7][8][9]. Within the framework of the fight against this pathogen, measures have been taken mainly to limit its spread by adopting a set of agricultural techniques [10].…”

Section: Introductionmentioning

confidence: 99%

Induction of Defense Gene Expression and the Resistance of Date Palm to Fusarium oxysporum f. sp. Albedinis in Response to Alginate Extracted from Bifurcaria bifurcata

et al. 2022

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In many African countries, the Bayoud is a common disease spread involving the fungus Fusarium oxusporum f. sp. albedinis (Foa). The induction of plant natural defenses through the use of seaweed polysaccharides to help plants against pathogens is currently a biological and ecological approach that is gaining more and more importance. In the present study, we used alginate, a natural polysaccharide extracted from a brown algae Bifurcaria bifurcata, to activate date palm defenses, which involve phenylalanine ammonia-lyase (PAL), a key enzyme of phenylpropanoid metabolism. The results obtained showed that at low concentration (1 g·L−1), alginate stimulated PAL activity in date palm roots 5 times more compared to the negative control (water-treated) after 24 h following treatment and 2.5 times more compared to the laminarin used as a positive stimulator of plant natural defenses (positive control of induction). Using qRT-PCR, the expression of a selection of genes involved in three different levels of defense mechanisms known to be involved in response to biotic stresses were investigated. The results showed that, generally, the PAL gene tested and the genes encoding enzymes involved in early oxidative events (SOD and LOX) were overexpressed in the alginate-treated plants compared to their levels in the positive and negative controls. POD and PR protein genes selected encoding β-(1,3)-glucanases and chitinases in this study did not show any significant difference between treatments; suggesting that other genes encoding POD and PR proteins that were not selected may be involved. After 17 weeks following the inoculation of the plants with the pathogen Foa, treatment with alginate reduced the mortality rate by up to 80% compared to the rate in control plants (non-elicited) and plants pretreated with laminarin, which agrees with the induction of defense gene expression and the stimulation of natural defenses in date palm with alginate after 24 h. These results open promising prospects for the use of alginate in agriculture as an inducer that triggers immunity of plants against telluric pathogens in general and of date palm against Fusarium oxysporum f. sp. albedinis in particular.

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Cited by 18 publications

References 16 publications

Pathogenic and molecular detection of <i>Fusarium oxysporum</i> f. sp. <i>albedinis</i> isolates from different areas in southwest Algeria

Pathogenic and molecular detection of <i>Fusarium oxysporum</i> f. sp. <i>albedinis</i> isolates from different areas in southwest Algeria

Chromosome-scale assembly uncovers genomic compartmentation of Fusarium oxysporum f. sp. albedinis, the causal agent of Bayoud disease in date palm

Induction of Defense Gene Expression and the Resistance of Date Palm to Fusarium oxysporum f. sp. Albedinis in Response to Alginate Extracted from Bifurcaria bifurcata

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