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Lin, H. S.; Jeu, M.J. de; Jacobsen, E.

doi:10.1023/a:1006063105940

Cited by 22 publications

(2 citation statements)

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“…Cytokinins play an important role in plant growth and development (Werner et al 2001), and participate in the maintenance of meristem function and in the modulation of metabolism and morphogenesis in response to environmental stimuli (Sakakibara et al 2006). However, appropriate plant genotype and physiological status of explants are crucial for an organogenic response (Bianchi et al 1999;Lin et al 1998;Magyar-Tábori et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Shoot regeneration from in vitro-derived leaf and root explants of Centaurea ultreiae

Mallón

Rodríguez-Oubiña

Fernández-González

2011

Plant Cell Tiss Organ Cult

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In vitro culture is currently used to produce plant material for ex situ conservation of endangered species. In this study, an efficient protocol for shoot regeneration from leaves and roots was developed for Centaurea ultreiae, a critically endangered species. Organogenesis from leaf and root explants was promoted by incubating these explants on half-strength Murashige and Skoog (MS) medium in the presence of one of four different cytokinins [6-benzyladenine (BA), zeatin, kinetin or N 6 -(2-isopentenyl) adenine (2iP)], each provided at five different levels. Shoot organogenesis was induced in both explants. The best response, 90% of leaf explants producing a mean of 2.48 shoots per explants and 94.3% of root explants producing a mean of 5.60 viable shoots per explants, was observed when explants were incubated on a medium containing 0.55 lM BA. Histological studies revealed connectivity between vascular tissues of regenerated shoots and cambial cells of leaf explants. Moreover, adventitious shoots were derived from pericycle cells of root explants and parenchymatic cells of callus tissues.

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Section: Introductionmentioning

confidence: 99%

“…Centaurea ultreiae Silva Pando is considered a critically endangered species in Spain (Bañares et al 2003) and is endemic to a small area of approximately 1,000 ha in R. Mallón (Izco et al 2003). Poor conservation of the habitat confines the species to slopes and edges of tracks.…”

Section: Introductionmentioning

confidence: 99%