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Abstract

The Hs1pro-1 gene confers resistance to the beet cyst nematode Heterodera schachtii in sugar beet (Beta vulgaris L.) on the basis of a gene-for-gene relationship. RNA-gel blot analysis revealed that the transcript of Hs1pro-1 was present in uninfected roots of resistant beet at low levels but increased by about fourfold one day after nematode infection. Treatments of plants with external stimuli including salicylic acid, jasmonic acid, gibberellic acid and abscisic acid as well as wounding or salt stress did not result in changes in the gene transcription, indicating de novo transcription of Hs1pro-1 upon nematode infection specifically. To study transcriptional regulation of Hs1pro-1 expression at the cellular level, a 3082 bp genomic fragment representing the Hs1pro-1 promoter, isolated from the YAC-DNA housing the Hs1pro-1 gene, was fused to the beta-glucuronidase reporter gene (1832prm1::GUS) and transformed into susceptible beet roots and Arabidopsis plants, respectively. Fluorometric and histochemical GUS assays on transgenic beet roots and Arabidopsis plants carrying the 1832prm1::GUS construct demonstrated that the Hs1pro-1 promoter is functional in both species and drives a nematode responsive and feeding site-specific GUS-expression. GUS activity was detected as early as at initiation of the nematode feeding sites and GUS staining was restricted to the nematode feeding sites. To delineate the regulatory domains of the Hs1pro-1 promoter, fusion genes with various 5' deletions of the Hs1pro-1 promoter and the GUS gene were constructed and analysed in transgenic beet roots as well. Cis elements responsible for feeding site-specific gene expression reside between -355 and +247 from the transcriptional initiation site of Hs1pro-1 whereas an enhancer region necessary for higher gene expression is located between -1199 and -705 of the promoter. The Hs1pro-1 promoter drives a nematode feeding site-specific GUS expression in both sugar beet and Arabidopsis suggesting a conserved mechanism of regulation of Hs1pro-1 expression in these two species.

Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem

“…The fifth DEG was Phvul.001G241300 that encodes an Hs1 pro-1 protein (Fig 5). This is interesting, as in sugar beet ( Beta vulgaris L.), Hs1 pro-1 has been demonstrated to confer resistance to the beet cyst nematode Heterodera schachtii through a gene-for-gene mechanism [62]. The sixth DEG was Phvul.001G242200 that encodes Cytochrome P450, that was up-regulated in resistant at 96 hpi.…”

RNA-Seq Analysis of a Soybean Near-Isogenic Line Carrying Bacterial Leaf Pustule-Resistant and -Susceptible Alleles

“…In sugar beet, the HS1 PRO-1 gene reportedly confers resistance to the beet cyst nematode. 68 Soybean homologues of HS1 PRO-1 were also expressed at high levels at 0 hai in the BLP-resistant NIL. These patterns suggest that a strong surveillance system exists in the BLP-resistant NIL, and that these genes play important roles in the modulation of defence responses to biotic and abiotic stress stimuli.…”

Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis

“…We also identified a homolog of ferritin that is induced in response to S. meliloti (TC39533); ectopic expression of ferritin results in a tolerance to oxidative damage and pathogens (48). Finally, the sugar beet (Beta vulgaris L.) nematode resistance gene is induced in response to nematode feeding (49,50) and is also induced in response to S. meliloti (TC38809).…”