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禎暢, 石井; 正人, 植村; 秀之, 小嶌; 辰一, 安; 伸也, 櫻井; 博, 福井

doi:10.2957/kanzo.42.455

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Dna Isolation and Amplification1

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“…The PCR profile was 3 min at 94°C followed by 35 cycles of 1 min at 94°C, 30 s at 48°C and 1 min at 72°C, and a final extension at 72°C for 10 min. Annealing temperature was set as low as 48°C to minimize taxonomic bias (following Ishii & Fukui 2001). Each sample was amplified in triplicate to avoid initial-cycle template bias.…”

Section: Dna Isolation and Amplificationmentioning

confidence: 99%

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Figure 3. Map of the 2016 reconstruction plan for Griend. A new sand suppletion was developed on the west side of the island (light grey) with shell ridges places on the borders (dark grey). Sod was removed in the center (blue), a washover (orange) was created in the north and dune ridges (green) were lowered on the dike in the north. (Photo: Cris Toala Olivares) Figure 4. The area that is recognized as the East Atlantic flyway is marked by the shaded area (Davidson & Pienkowski 1987) Chapter 2 Sanderling diet in the Wadden Sea 24

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Section: Dna Isolation and Amplificationmentioning

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