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Juengel, Jennifer L.; Bibby, Adrian H; Reader, Karen L; Lun, S.; Quirke, Laurel D.; Haydon, Lisa J; McNatty, Kenneth P.

doi:10.1186/1477-7827-2-78

Cited by 54 publications

(36 citation statements)

References 54 publications

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“…The intra-and inter-assay coefficients of variation (CV) for three standard sample assayed at approximately 20, 50 or 80% binding both averaged !20% and no samples were below the sensitivity of the assay. At the end of culture, following collection of the media, unattached cells were removed by washing, and attached cells were lysed using incubation at 37 8C in distilled water for 1-2 h. Cell lysates were frozen at K70 8C until assayed for DNA content using a Hoechst 33258 based dye assay (Juengel et al 2004). Intra-and inter-assay CV were !10%, and no samples were below the sensitivity of the assay.…”

Section: Culture Of Luteinising Ovine Granulosa Cellsmentioning

confidence: 99%

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Activin A and follistatin during the oestrous cycle and early pregnancy in ewes

O’Connell¹,

McNatty²,

Hurst³

et al. 2016

Journal of Endocrinology

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The activin pathway has been postulated to be involved in regulation of multiple reproductive processes important for survival of the conceptus. These processes include luteinisation of the follicular cells and thus function of the corpus luteum, early embryo development and uterine function including implantation of the conceptus. Therefore, the aim of the current study was to determine whether the concentrations of activin A and follistatin (FST), an activin-binding protein, differed between ewes with a lifetime history of enhanced or reduced embryonic survival (ES). The mRNAs encoding FST and activin A (inhibin beta A subunit; INHBA) were present in the uterus and abundant in the uterine luminal or glandular epithelia by day 18 of gestation. A peak of activin A was observed in the systemic circulation around the time of oestrus, and activin A concentrations were elevated in animals with reduced ES during the oestrous cycle and early gestation. Concentrations of activin A in uterine fluid were approximately twofold greater on day 16 of gestation in ewes with reduced ES compared to those with enhanced ES. No consistent differences in FST were observed between these groups. Treatment of luteinising ovine granulosa cells with activin A in vitro suppressed progesterone secretion providing evidence of a potential pathway whereby increased concentrations of activin A may decrease ES.

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Section: Culture Of Luteinising Ovine Granulosa Cellsmentioning

confidence: 99%

“…Collection and culture of ovine granulosa cells were as previously described (Juengel et al 2004). Briefly, ovaries were collected from the local abattoir and transported back to the laboratory.…”

Section: Culture Of Luteinising Ovine Granulosa Cellsmentioning

confidence: 99%

Activin A and follistatin during the oestrous cycle and early pregnancy in ewes

O’Connell¹,

McNatty²,

Hurst³

et al. 2016

Journal of Endocrinology

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“…TGFB1 has also been detected in oocytes of goat preantral follicles (Rodrigues et al, 2014). TGFB1 and 2 both reduced numbers of granulosa cells after culture in sheep (Juengel et al, 2004a), although no effect of TGFB1 on proliferation was observed (TGFB2 was not tested). In cattle, the effects of TGFB are inconsistent, with TGFB2 having a mild stimulatory effect on proliferation (Gilchrist et al, 2003), and TGFB (type unspecified) having no effect on unstimulated, or an inhibitory effect on epidermal growth factor (EGF)-stimulated, proliferation (Skinner et al, 1987).…”

Section: Introductionmentioning

confidence: 98%

“…In sheep, TGFB1 and TGFB2, but not TGFB3, are produced by ovarian follicles, in the theca cells of type 3 and larger follicles (Juengel et al, 2004a). In cattle, TGFB1, 2 and 3 were all detected in oocytes, granulosa and theca cells, with TGFB3 being the most strongly expressed (Nilsson et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Please see text for more details. Information on expression of proteins was gathered from multiple references (Bezard et al, 1987;Wandji et al, 1992;Braw-Tal, 1994;Tisdall et al, 1994Tisdall et al, , 1995Tisdall et al, , 1997Leeuwenberg et al, 1995;Xu et al, 1995;Logan et al, 2002Logan et al, , 2003Juengel et al, 2004aJuengel et al, , 2006a The effects of TGFB superfamily members on granulosa cell proliferation and health (Fig. 2) Two oocyte-secreted growth factors from the TGFB superfamily, GDF9 and BMP15, are known to be essential for normal granulosa cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

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The local regulation of folliculogenesis by members of the transforming growth factor superfamily and its relevance for advanced breeding programmes

et al. 2018

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Regulation of the growth and maturation of the ovarian follicle is critical for normal reproductive function. Alterations in this growth can lead to pathological conditions, such as cystic follicles, reduced oocyte quality, or an abnormal endocrine environment leading to poor fertility. Alterations in follicular growth also influence the number of follicles ovulating and thus can change litter size. Both endocrine factors, such as follicle stimulating hormone and luteinizing hormone, as well as local factors, are known to regulate follicular growth and development. This review will focus on the role of local factors in regulation of ovarian follicular growth in ruminants, with a focus on members of the transforming growth factor superfamily. The potential role of these factors in regulating proliferation, apoptosis, steroidogenesis and responsiveness to gonadotrophins will be considered.

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Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats

Cheng

Sun

et al. 2014

Birth Defects Research Pt B

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This study was carried out to investigate the impact of tripterygium glycosides (TGs) on ovarian function of female rats in vitro and in vivo. In vitro studies showed that TG induced cells decrease at G1 phase and inhibited cell proliferation in rat granulosa cells. In vivo, female rats were intragastrically administered with TG at the dose of 60 mg/kg/day for consecutive 50 days. TG caused a prolonged estrous cycle, and a significant reduction in ovarian index, serum E2 level, and numbers of secondary and antral follicles (p < 0.05) in these rats. A significant reduction of viable embryos was demonstrated in TG-treated female rats after mating (p < 0.01). Further, we observed observed the reduced expression level of TGF-β1 after TG treatment in vitro and in vivo. Moreover, the expression of Smad2 and AKT was also decreased after TG treatment. These results suggest that TG can impair ovarian function through Smads-mediated TGF-β1 signal pathway.

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Abstract: Background: Recently, several members of the transforming growth factor-beta (TGF-beta) superfamily have been shown to be essential for regulating the growth and differentiation of ovarian follicles and thus fertility.

Cited by 54 publications

References 54 publications

Activin A and follistatin during the oestrous cycle and early pregnancy in ewes

Activin A and follistatin during the oestrous cycle and early pregnancy in ewes

The local regulation of folliculogenesis by members of the transforming growth factor superfamily and its relevance for advanced breeding programmes

Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats

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