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Kawai, Tomoji; SAKATA, Tadayoshi; Hashimoto, Kazuhito; KAWAI, Maki

doi:10.1246/nikkashi.1984.277

Cited by 7 publications

(1 citation statement)

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“…Enzymes hydrolysing (1 -. 3)-,d-D-glucans occur widely in filamentous fungi [1][2][3][4][5][6]. They can be classified, according to their action on linear (1 -.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Pitson

Seviour

McDougall

et al. 1995

Biochemical Journal

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Three (1-->3)-beta-D-glucanases (GNs) were isolated from the culture filtrates of the filamentous fungus Acremonium persicinum and purified by (NH4)2SO4 precipitation followed by anion-exchange and gel-filtration chromatography. Homogeneity of the purified proteins was confirmed by SDS/PAGE, isoelectric focusing and N-terminal amino acid sequencing. All three GNs (GN I, II and III) are non-glycosylated, monomeric proteins with apparent molecular masses, estimated by SDS/PAGE, of 81, 85 and 89 kDa respectively. pI values for the three enzymes are 5.3, 5.1, and 4.4 respectively. The pH optimum for GN I is 6.5, and 5.0 for GN II and III. All three purified enzymes displayed stability over the pH range 4.5-10.0. Optimum activities for GN I, II and III were recorded at 65, 55 and 60 degrees C respectively, with both GN II and III having short-term stability up to 50 degrees C and GN I up to 55 degrees C. The purified GNs have high specificity for (1-->3)-beta-linkages and hydrolysed a range of (1-->3)-beta- and (1-->3)(1-->6)-beta-D-glucans, with laminarin from Laminaria digitata being the most rapidly hydrolysed substrate of those tested. K(m) values for GN I, II, and III against L. digitata laminarin were 0.1, 0.23 and 0.22 mg/ml respectively. D-Glucono-1,5-lactone does not inhibit any of the three GNs, some metals ions are mild inhibitors, and N-bromosuccinimide and KMnO4 are strong inhibitors. All three GNs acted in an exo-hydrolytic manner, determined by the release of alpha-glucose as the initial and major product of hydrolysis of (1-->3)-beta-D-glucans, and confirmed by viscometric analysis and the inability to cleave periodate-oxidized laminarin, and may be classified as (1-->3)-beta-D-glucan glucohydrolases (EC 3.2.1.58).

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“…Enzymes hydrolysing (1 -. 3)-,d-D-glucans occur widely in filamentous fungi [1][2][3][4][5][6]. They can be classified, according to their action on linear (1 -.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Pitson

Seviour

McDougall

et al. 1995

Biochemical Journal

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Separation Mechanism of a Photoinduced Electron‐Hole Pair in Metal‐Loaded Semiconductor Powders

Nosaka

Ishizuka

Miyama

1986

Ber Bunsenges Phys Chem

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Metals J Photochemistry 1 Photoelectrochemistry J Semiconductors J SurfacesPhotocatalytic activities of metal loaded CdS and p-GaP semiconductor powders were measured for ammonia production from aqueous N, solution and hydrogen evolution from ethanol-water mixture. The higher activity was obtained for the CdS loaded with the metal having a higher work function. On the other hand, the metal-loading on p-GaP powders showed a slight increase in the activity with loading lower work function metals. The numerical calculation for two-dimensional Poisson equation shows that the electric field gradient is formed around the metal spots. This field gradient causes electrons to be captured by the metal, and holes to be collected on the semiconductor-solution interface between the metal spots.

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Photolysis of aqueous poly(vinyl alcohol) solution by heterogeneous TiO₂/Pt catalyst

Nishimoto

Ohtani

Shirai

et al. 1985

J. Polym. Sci. B Polym. Lett. Ed.

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Cited by 7 publications

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Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Separation Mechanism of a Photoinduced Electron‐Hole Pair in Metal‐Loaded Semiconductor Powders

Photolysis of aqueous poly(vinyl alcohol) solution by heterogeneous TiO₂/Pt catalyst

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Untitled

Cited by 7 publications

References 0 publications

Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Purification and characterization of three extracellular (1→3)-β-d-glucan glucohydrolases from the filamentous fungus Acremonium persicinum

Separation Mechanism of a Photoinduced Electron‐Hole Pair in Metal‐Loaded Semiconductor Powders

Photolysis of aqueous poly(vinyl alcohol) solution by heterogeneous TiO2/Pt catalyst

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Photolysis of aqueous poly(vinyl alcohol) solution by heterogeneous TiO₂/Pt catalyst