Untitled

Abstract

Nuclear magnetic resonance spectroscopy is an absorption spectroscopic technique that helps in structural elucidation and has a wide range of applications in various fields like drug discovery and development, metabolomics, combinatorial chemistry, and food sciences. This paper gives information about nuclear magnetic resonance spectroscopy, and clear description of LC-NMR-MS, and a brief description of other hyphenations with NMR like HPLC-NMR, GC-NMR, GPC-NMR, SEC-NMR, SFC-NMR, SFE-NMR, CE-NMR, SPE-NMR, CEC-NMR, and their applications. INTRODUCTION: Nuclear Magnetic Resonance Spectroscopy: Nuclear Magnetic Resonance (NMR) spectroscopy is an absorption spectroscopic technique where the energy from an external source is absorbed and brings about resonance to an excited or higher energy state (in which nuclei in a strong constant magnetic field are perturbed by an oscillating magnetic field). The resonance occurs when the oscillation frequency matches the intrinsic frequency of the nuclei. In NMR, the energy required lies in the low energy or long-wavelength radio-frequency end of the electromagnetic spectrum. The emerging radiofrequency gets absorbed in a magnetic field consequent to the magnetic properties of nuclei arising from the axial spin.