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Y, Ko; Koch, Brigitte; Sachinidis, Agapios; Thier, Ricarda; Vetter, Hans; Hm, Bolt; Brüning, Thomas

doi:10.1097/00008571-200004000-00009

Cited by 54 publications

(9 citation statements)

References 10 publications

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“…In addition to the polymorphism of CYP1A1, also the CYP1B1 codon 432 polymorphism (CYP1B1*3) has been identified as a susceptibility factor in smoking-related head and neck squameous cell cancer [96,97]. The coherence was supported by an association with the frequency of somatic mutations of the p53 gene.…”

Section: Head and Neck Cancermentioning

confidence: 99%

Relevance of the Deletion Polymorphisms of the Glutathione S-Transferases GSTT1 and GSTM1 in Pharmacology and Toxicology

Bolt

Thier²

2006

CDM

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257

181

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Although cytosolic glutathione S-transferase (GST) enzymes occupy a key position in biological detoxification processes, two of the most relevant human isoenzymes, GSTT1-1 and GSTM1-1, are genetically deleted (non-functional alleles GSTT1*0 and GSTM1*0) in a high percentage of the human population, with major ethnic differences. The structures of the GSTT and GSTM gene areas explain the underlying genetic processes. GSTT1-1 is highly conserved during evolution and plays a major role in phase-II biotransformation of a number of drugs and industrial chemicals, e.g. cytostatic drugs, hydrocarbons and halogenated hydrocarbons. GSTM1-1 is particularly relevant in the deactivation of carcinogenic intermediates of polycyclic aromatic hydrocarbons. Several lines of evidence suggest that hGSTT1-1 and/or hGSTM1-1 play a role in the deactivation of reactive oxygen species that are likely to be involved in cellular processes of inflammation, ageing and degenerative diseases. There is cumulating evidence that combinations of the GSTM1*0 state with other genetic traits affecting the metabolism of carcinogens (CYP1A1, GSTP1) may predispose the aero-digestive tract and lung, especially in smokers, to a higher risk of cancer. The GSTM1*0 status appears also associated with a modest increase in the risk of bladder cancer, consistent with a GSTM1 interaction with carcinogenic tobacco smoke constituents. Both human GST deletions, although largely counterbalanced by overlapping substrate affinities within the GST superfamily, have consequences when the organism comes into contact with distinct man-made chemicals. This appears relevant in industrial toxicology and in drug metabolism.

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Section: Head and Neck Cancermentioning

confidence: 99%

Relevance of the Deletion Polymorphisms of the Glutathione S-Transferases GSTT1 and GSTM1 in Pharmacology and Toxicology

Bolt

Thier²

2006

CDM

Self Cite

257

181

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“…However, these techniques require expensive hybridization probes or complex calculations of the copy-number variation and cannot determine GST-T1 and GST-M1 genotypes simultaneously in one reaction mixture. 27-29 …”

Section: Discussionmentioning

confidence: 99%

“…Recently, other techniques using real-time PCR for GST-T1 and GST-M1 null genotype evaluation have been implemented. 27-30 Gene specific hybridization probes have been used to identify the carriers of at least one functional GST-T1 or GST-M1 allele and to distinguish them from the individuals who are homozygous for the GST-T1 or GST-M1 null genotype. 27 A quantitative real-time PCR method has been developed to evaluate the copy number polymorphisms, a trimodal genotype (+/+, +/−, and −/−) for GST-T1 or GST-M1 .…”

Section: Introductionmentioning

confidence: 99%

“…27-30 Gene specific hybridization probes have been used to identify the carriers of at least one functional GST-T1 or GST-M1 allele and to distinguish them from the individuals who are homozygous for the GST-T1 or GST-M1 null genotype. 27 A quantitative real-time PCR method has been developed to evaluate the copy number polymorphisms, a trimodal genotype (+/+, +/−, and −/−) for GST-T1 or GST-M1 . 28,29 However, like the method that uses gene specific hybridization probes, 27 this technique cannot analyze both genes, GST-T1 and GST-M1, in one reaction vessel.…”

Section: Introductionmentioning

confidence: 99%

“…27 A quantitative real-time PCR method has been developed to evaluate the copy number polymorphisms, a trimodal genotype (+/+, +/−, and −/−) for GST-T1 or GST-M1 . 28,29 However, like the method that uses gene specific hybridization probes, 27 this technique cannot analyze both genes, GST-T1 and GST-M1, in one reaction vessel.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the GST-T1 and GST-M1 Null Genotypes Using High Resolution Melting Analysis

Drobná

Razo

Garcia-Vargas

et al. 2011

Chem. Res. Toxicol.

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Glutathione S-transferases, including GST-T1 and GST-M1, are known to be involved in the phase II detoxification pathways for xenobiotics as well as in the metabolism of endogenous compounds. Polymorphisms in these genes have been linked to an increased susceptibility to carcinogenesis and associated with risk factors that predispose to certain inflammatory diseases. In addition, GST-T1 and GST-M1 null genotypes have been shown to be responsible for interindividual variations in metabolism of arsenic, a known human carcinogen. To assess the specific GST genotypes in the Mexican population chronically exposed to arsenic, we have developed a multiplex High Resolution Melting PCR (HRM-PCR) analysis using LightCycler480 instrument. This method is based on analysis of the PCR product melting curve that discriminates PCR products according to their lengths and base sequences. Three pairs of primers that specifically recognize GST-T1, GST-M1, and β-globin, an internal control, to produce amplicons of different length were designed and combined with LightCycler480 High Resolution Melting Master Mix containing ResoLight, a completely saturating DNA dye. Data collected from melting curve analysis were evaluated using LightCycler480 software to determine specific melting temperatures of individual melting curves representing target genes. Using this newly developed multiplex HRM-PCR analysis we evaluated GST-T1 and GST-M1 genotypes in 504 DNA samples isolated from blood of individuals residing in Zimapan, Lagunera, and Chihuahua regions in Mexico. We found that Zimapan and Lagunera populations have similar GST-T1 and GST-M1 genotype frequencies which differ from Chihuahua population. In addition, 14 individuals have been identified as carriers of double null genotype, i.e. null genotypes in both GST-T1 and GST-M1 genes. Although this procedure does not distinguish between biallelic (+/+) and monoallelic (+/−) genotypes it can be used in an automated workflow as a simple, sensitive, time and money saving procedure for rapid identification of the GST-T1 and GST-M1 positive or null genotypes.

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Glutathion‐S‐transferase P1 (Genotypisierung) [Biomonitoring Methods in German language, 2004]

Rihs¹,

Brüning²

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Analytische Methoden zur Prüfung gesundheitsschädlicher Arbeitsstoffe: Analysen in biologischem Material , 16. Lieferung, Ausgabe 2004 Der Artikel enthält folgende Kapitel: Grundlage des Verfahrens Geräte, Chemikalien, Lösungen, Gele Geräte Chemikalien Lösungen Gele Probenaufarbeitung Lagerung DNA‐Isolierung Bestimmung der Reinheit und Konzentration der DNA Vorbereitung und Durchführung der PCR PCR Restriktionsschnitte der PCR Elektrophorese der Restriktionsfragmente Kalibrierung Auswertung Qualitätskontrolle Störeinflüsse Diskussion der Methode

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Untitled

Cited by 54 publications

References 10 publications

Relevance of the Deletion Polymorphisms of the Glutathione S-Transferases GSTT1 and GSTM1 in Pharmacology and Toxicology

Relevance of the Deletion Polymorphisms of the Glutathione S-Transferases GSTT1 and GSTM1 in Pharmacology and Toxicology

Identification of the GST-T1 and GST-M1 Null Genotypes Using High Resolution Melting Analysis

Glutathion‐S‐transferase P1 (Genotypisierung) [Biomonitoring Methods in German language, 2004]

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