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Berzonsky, William A.; Francki, Michael G.

doi:10.1023/a:1003638131743

Cited by 29 publications

(6 citation statements)

References 138 publications

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“…segment. Therefore, we concluded that the positions of wheat-rye chromosome breakpoints can be accurately determined by a combination of in situ hybridization and phenotypic, molecular, and biochemical marker data as originally proposed by Berzonsky and Francki (1999).…”

Section: Discussionmentioning

confidence: 74%

“…Primary, secondary, and tertiary recombinants involving 1DS and 1RS were characterized using molecular markers and in situ hybridization. The initial marker used in this study was the 350-480 bp rye telomeric repetitive sequence pAW161 (Guidet et al 1991;Berzonsky and Francki 1999), to confirm the absence of the telomeric sequences in the secondary and tertiary recombinants. Figure 4 shows the presence of the rye-specific telomeric sequence in the original 1DL.1RS translocation and in primary recombinant I-93, but this sequence was absent in primary recombinant 82-180.…”

Section: Characterization Of Recombinants Using Molecular Markers Andmentioning

confidence: 99%

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Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Anugrahwati

Shepherd

Verlin

et al. 2008

Genome

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Chromosome 1R of rye is a useful source of genes for disease resistance and enhanced agronomic performance in wheat. One of the most prevalent genes transferred to wheat from rye is the stem rust resistance gene Sr31. The recent emergence and spread of a stem rust pathotype virulent to this gene has refocused efforts to find and utilize alternative sources of resistance. There has been considerable effort to transfer a stem rust resistance gene, SrR, from Imperial rye, believed to be allelic to Sr31, into commercial wheat cultivars. However, the simultaneous transfer of genes at the Sec-1 locus encoding secalin seed storage proteins and their association with quality defects preclude the deployment of SrR in some commercial wheat breeding programs. Previous attempts to induce homoeologous recombination between wheat and rye chromosomes to break the linkage between SrR and Sec-1 whilst retaining the tightly linked major loci for wheat seed storage proteins, Gli-D1 and Glu-D3, and recover good dough quality characteristics, have been unsuccessful. We produced novel tertiary wheat-rye recombinant lines carrying different lengths of rye chromosome arm 1RS by inducing homoeologous recombination between the wheat 1D chromosome and a previously described secondary wheat-rye recombinant, DRA-1. Tertiary recombinant T6-1 (SrR+ Sec-1-) carries the target gene for stem rust resistance from rye and retains Gli-D1 but lacks the secalin locus. The tertiary recombinant T49-7 (SrR- Sec-1+) contains the secalin locus but lacks the stem rust resistance gene. T6-1 is expected to contribute to wheat breeding programs in Australia, whereas T49-7 provides opportunities to investigate whether the presence of secalins is responsible for the previously documented dough quality defects.

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Section: Discussionmentioning

confidence: 74%

Section: Characterization Of Recombinants Using Molecular Markers Andmentioning

confidence: 99%

Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Anugrahwati

Shepherd

Verlin

et al. 2008

Genome

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“…The best-known examples are the interspeci c 1R(1B) chromosome substitutions and the 1RS.1BL translocations introduced from cultivated rye into hexaploid wheat [49,50]. While the translocation can be diagnosed by various biochemical and molecular methods [51], the initial molecular cytogenetic evidence for the 1RS.1BL translocation was provided by GISH [52]. Here, we used, for the rst time, immunoFISH-GISH to analyse another Robertsonian translocation (7HS.7DL [53]) and demarcated the introgression by its functional boundaries, i.e., the active centromere via immunolabelling and the telomeric end via FISH.…”

Section: Discussionmentioning

confidence: 99%

Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Makai

Mihók

Polgári

et al. 2023

Preprint

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Background Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situdetection of proteins and nucleic acids are still sporadic in plant cell biology. A major bottleneck has been the availability of high-quality plant nuclei with a balance between preservation of 3D ultrastructure and maintaining immunoreactivity. The aim of this study was to develop a quick and reliable procedure to prepare plant nuclei suitable for various combinations of immunolabelling and fluorescence in situ hybridisation methods (immunoFISH-GISH). Results The mechanical removal of the cell wall and cytoplasm, instead of enzymatic degradation, resulted in a gentle, yet effective, cell permeabilisation. Rather than manually releasing the nuclei from the fixed tissues, the procedure involves in-solution cell handling throughout the fixation and the preparation steps as ended with pipetting the pure nuclei suspension onto microscope slides. The optimisation of several critical steps is described in detail. Finally, the procedure is shown to be compatible with immunolabelling, FISH and GISH as well as their simultaneous combinations. Conclusion A simple plant cell nuclei preparation procedure was developed for combined immunolabelling-in situ hybridisation methods. The main and critical elements of the procedure are: a short period of fixation, incorporation of detergents to facilitate the fixation of tissues and the penetration of probes, tissue grinding to eliminate unwanted cell components, and an optimal buffer to handle nuclei. The procedure is time efficient and is easily transferable without prior expertise.

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“…For instance, a quick search for Robertsonian translocations revealed some 40 cases in wheat in combination with species from ten different genera, including Hordeum (five cases), Secale (9), and Thinopyrum (15). All these translocations were either meiotically produced (e.g., wheat–barley: Danilova et al 2018; Türkösi et al 2018) or spontaneously generated and discovered in advanced progenies (Thomas et al 1998; Berzonsky and Francki 1999). Since the developmental stage (Zadoks scale: Z13–14 or 3–4 leaf stage) of our primary plant material was deliberately chosen to set it well before the first meiosis, only the possibility of somatic translocations might still left to be considered.…”

Section: Discussionmentioning

confidence: 99%

Composition and random elimination of paternal chromosomes in a large population of wheat × barley (Triticum aestivum L. × Hordeum vulgare L.) hybrids

2019

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Key message Statistical analysis of the chromosomal composition in a population of 210 primary plants regenerated from two intergeneric wheat–barley cross combinations revealed the random nature of uniparental elimination for barley chromosomes. Abstract Uniparental chromosome elimination is a common process in interspecific and intergeneric cereal hybrids. To characterize the frequency of paternal chromosomes, a population of 218 independent green plants was generated from two wheat (♀) × barley (♂) cross combinations via embryo rescue. The chromosomal composition of 210 primary plants was analyzed with chromosome-specific DNA markers representing all seven barley chromosomes. The analysis revealed an equal proportion of haploid and full hybrids (20.5% and 19.5%, respectively), while the rest of the population contained hypoploids (partial hybrids) with no preference for any possible numbers (one to six) of barley chromosome additions. Contrary to the previous reports, there was no statistical bias or preferential elimination for any individual barley chromosome (1H–7H) in this population. The reasons for the apparent contradiction and the implications of the above findings for cereal breeding are discussed. Electronic supplementary material The online version of this article (10.1007/s00299-019-02405-1) contains supplementary material, which is available to authorized users.

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Cited by 29 publications

References 138 publications

Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Composition and random elimination of paternal chromosomes in a large population of wheat × barley (Triticum aestivum L. × Hordeum vulgare L.) hybrids

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