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Ghosh, Srikant; Khan, M.H.; Ahmed, Nizamuddin

doi:10.1023/a:1005218821889

Cited by 20 publications

(7 citation statements)

References 17 publications

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“…The immunogenicity of these proteins could be a result of their positions as membrane proteins. The reaction of these GLPs with the hyperimmune sera from rabbits would confirm the fact that unfed larvae provides an easier source of biological material for the isolation of protective antigens, and is consistent with the data collected by Ghosh et al [10,11] and Ghosh and Khan [9]. …”

Section: Discussionsupporting

confidence: 89%

“…The immunoaffinity chromatographic purification method was previously attempted for the purification of salivary gland antigens of Amblyomma americanum [4], larval antigens [9,10,12], gut origin larval antigen [6], and nymphal antigen [22] of Hyalomma anatolicum . In the case of B. microplus , 3 antigens with molecular weights of 86 kDa [20], 63 kDa [15], and 75-80 kDa [7] were isolated in pure form and tested for their protective potentiality.…”

Section: Discussionmentioning

confidence: 99%

“…The other Australian scientists used 500 µg affinity-purified larval antigens per animal [30]. In the case of H. anatolicum , 2 mg of larval origin 39 kDa protein [9,10], 1.6 mg of gut origin larval antigens [6], and 1.6 mg of nymphal origin of 39 kDa protein [22] were used and found to be protective against homologous challenge.…”

Section: Discussionmentioning

confidence: 99%

“…Whole tick and larval antigens of B. annulatus were constructed according to the method of Ghosh et al [9,10]. In brief, laboratory-reared, clean, 5- to 6-day-old unfed ticks or larvae were homogenized in cold 0.15M phosphate-buffered saline (PBS) and 1mM disodium EDTA, pH 7.2, containing cocktail protease inhibitors, and were then filtered, sonicated, and centrifuged at 15,000 × g for 60 min at 4℃.…”

Section: Methodsmentioning

confidence: 99%

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Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

et al. 2007

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The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

et al. 2007

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“…For H. anatolicum , 2 mg of larval 39 kDa protein [12,13], 1.6 mg of gut larval antigens [8], and 1.6 mg of nymphal 39 kDa protein [23] were found to be protective against homologous challenge. In the present investigation, a biochemical approach was used to purify the major GLPs of the H. dromedarii tick.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine

et al. 2011

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In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 µg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The effects of immunity induced by these GLPs were determined by exposing rabbits to adult H. dromedarii ticks. These results demonstrated that GLP immunization led to a slightly decreased reproductive index and significantly reduced rates of egg hatchability. These results demonstrated that immunization with the purified GLPs can provide protection against infestation by H. dromedarii and some other tick species. Further studies are needed to confirm the effectiveness of immunization with GLPs against other tick species.

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Assessment of duration of immunity in crossbred cattle immunized with glycoproteins isolated from Hyalomma anatolicum anatolicum and Boophilus microplus

2005

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To develop immunoprophylactic measures against multi-tick infestation, two glycoproteins of 34 and 29 kDa were isolated from the larvae of Hyalomma anatolicum anatolicum and Boophilus microplus, respectively, and assessed for their efficacy against experimental challenge infestations. The synergistic effect of the antigens in the presence of incomplete Freund's adjuvant was found to confer protection (DT%) in animals against 56.48% of larvae and 52% of adults of H. a. anatolicum, while the effect was 40% against adults of B. microplus. The efficacy (E%) of the antigens in combination against larvae and adults of H. a. anatolicum was calculated as 70% and 64.3%, respectively, and 63% against adults of B. microplus. A direct correlation between anti-glycoprotein antibody response and protection against infestation was observed. Western blot analysis detected specific antigen in the sera of animals of group A. The antigens in combination with incomplete Freund's adjuvant could protect animals from H. a. anatolicum and B. microplus infestations for at least 30 weeks. The possibility of employing the vaccination strategy in Indian conditions is discussed.

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Cited by 20 publications

References 17 publications

Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine

Assessment of duration of immunity in crossbred cattle immunized with glycoproteins isolated from Hyalomma anatolicum anatolicum and Boophilus microplus

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