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Nicklin, Paul; Bayley, Deborah; Giddings, June; Craig, S. J.; Cummins, Lendell L.; Hastewell, J G; Phillips, J. A.

doi:10.1023/a:1011934011690

Cited by 55 publications

(5 citation statements)

References 20 publications

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“…Similar results were obtained when 30 μ g or 10 μ g of RNA were fed to mice (Table S2). The small percentage of dietary RNA detected indicates a low level of uptake, and is consistent with other recent works (Nicklin et al 1998; Snow et al 2013). …”

Section: Discussionsupporting

confidence: 92%

Assessing the survival of exogenous plant microRNA in mice

Liang

Zhu

Sun

et al. 2014

Food Science & Nutrition

122

107

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MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10–50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05–4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.

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Section: Discussionsupporting

confidence: 92%

Assessing the survival of exogenous plant microRNA in mice

Liang

Zhu

Sun

et al. 2014

Food Science & Nutrition

122

107

View full text Add to dashboard Cite

show abstract

“…Thus, a subtype-specific inhibitor would be required for the functional analysis of oatps. It is known that AONs are distributed mainly to the liver and kidney, less so to skeletal muscles, and not at all to the brain ( , ). For the analysis of oatps in the liver and kidney, subtype-specific oatp ENA AONs, such as OA1-9, OA2-13c, and OA3-2c, which were designed and constructed in this study, might be useful in vivo .…”

Section: Resultsmentioning

confidence: 99%

Enhancement of the Inhibitory Activity of oatp Antisense Oligonucleotides by Incorporation of 2‘-O,4‘-C-Ethylene-Bridged Nucleic Acids (ENA) without a Loss of Subtype Selectivity

et al. 2004

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Antisense oligonucleotides (AONs) that specifically target the genes of rat organic anion transporting polypeptide (oatp) subtypes were selected by using antisense in vitro selection (AIVS) and a conventional gene alignment program (GAP). When we incorporated several of our original 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) residues into AONs, which were designed as gapmers containing a series of 2'-deoxynucleotides in the center, at both the 3' and 5' ends, the inhibitory activity of these oatp AONs was enhanced and their inhibition was mediated by RNase H cleavage. Moreover, these ENA AONs did not lose their oatp selectivity. These strategies of using AIVS and GAP to select AONs followed by incorporation of ENA residues were effective for synthesizing oatp subtype-specific AONs.

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“…Thus, the systemic absorption of orally ingested RNA is negligible. For a 20mer DNA oligonucleotide, constructed with stabilizing phosphorothionate linkages to prevent degradation in the gastrointestinal tract, oral bioavailability in rats was at best 0.3% after gavage administration (Nicklin et al, 1998). Kendal D. Hirschi and colleagues reported on an unusual sRNA from herbs, flowers and vegetables with a comparably high stability in gastrointestinal fluids (Yang et al, 2016(Yang et al, , 2018.…”

Section: Oral Exposure From Dietary Intakementioning

confidence: 99%

RNAi for plant improvement and protection

Mezzetti¹,

Sweet²,

Burgos³

2021

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This book contains a series of chapters reviewing the current scientific knowledge on RNAi, methods for developing RNAi systems in transgenic plants and a range of applications for crop improvement, crop production and crop protection. Some chapters examine both endogenous systems in transgenic plants and exogenous systems where interfering RNAs are applied to target plants, pests and pathogens. The biosafety of these different systems is examined and methods for risk assessment for food, feed and environmental safety are discussed. Finally, aspects of the regulation of technologies exploiting RNAi and the socioeconomic impacts of RNAi technologies are discussed.

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Untitled

Cited by 55 publications

References 20 publications

Assessing the survival of exogenous plant microRNA in mice

Assessing the survival of exogenous plant microRNA in mice

Enhancement of the Inhibitory Activity of oatp Antisense Oligonucleotides by Incorporation of 2‘-O,4‘-C-Ethylene-Bridged Nucleic Acids (ENA) without a Loss of Subtype Selectivity

RNAi for plant improvement and protection

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