Untitled

Ain‐Lhout, F.; Zunzunegui, María; Barradas, Mari Cruz Díaz; Tirado, Reyes; Clavijo, A.; Novo, Francisco García

doi:10.1023/a:1010387610098

Cited by 123 publications

(21 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, although the reported plant response is increased proline production, the level of the produced proline is considerably different in different species and/or in sensitive and tolerant cultivars. Furthermore, it was reported that proline accumulation during osmotic stress is higher in sensitive than in the tolerant wheat genotypes [34] and this observation is in accordance with data reported for other species such as cassava [35]; two Mediterranean shrubs [36]; beech [37] and barley [38]. Therefore we could conclude, based on the reported general observations that the sensitive species respond to osmotic stress by accumulating higher levels of proline; furthermore, in the sensitive genotypes osmotic stress induced proline level are higher than in the tolerant one.…”

Section: Discussionsupporting

confidence: 88%

The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

2010

View full text Add to dashboard Cite

BackgroundThe overall research objective was to develop single cell plant cultures as a model system to facilitate functional genomics of monocots, in particular wheat and barley. The essential first step towards achieving the stated objective was the development of a robust, viable single cell suspension culture from both species.ResultsWe established growth conditions to allow routine culturing of somatic cells in 24 well microtiter plate format. Evaluation of the wheat and barley cell suspension as model cell system is a multi step process. As an initial step in the evaluation procedure we chose to study the impact of selected abiotic stress elicitors at the physiological, biochemical and molecular level. We report the results of osmotic stress imposed by NaCl and PEG. As proline is an important osmoprotectant of the cereal cells, colorimetric assay for proline detection was developed for small volumes (200 μl). We performed RT-PCR experiments to study the change in the expression of the genes encoding Δ1-pyrroline-5-carboxylate synthetase (P5CS) and Δ1-pyrroline-5-carboxylate reductase (PC5R) in response to abiotic stress.ConclusionsWe found differences between the wheat and barley suspension cultures, barley being more tolerant to the applied osmotic stresses. We suggested a model to explain the obtained differences in stress tolerance between the two species. The suspension cell cultures have proven useful for determining changes in proline concentration and expression level of genes (P5CS, P5CR) under various treatments and we suggest that the cells can be used as a model host system to study gene expression and regulation in monocots.

show abstract

Section: Discussionsupporting

confidence: 88%

The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

2010

View full text Add to dashboard Cite

show abstract

“…Proline could function as a hydroxyl radical scavenger to prevent membrane damage and protein denaturation (Ain-Lhout et al 2001). ASA was another non-enzymatic antioxidant acting as a substrate for extracellular enzymes for attenuating AOS levels (Burkey et al 2006).…”

Section: Warming Effects Of Infrared Heatersmentioning

confidence: 99%

Growth and physiological responses of Picea asperata seedlings to elevated temperature and to nitrogen fertilization

Zhao

Liu

2008

Acta Physiol Plant

View full text Add to dashboard Cite

Picea asperata is a dominant species in the subalpine coniferous forests distributed in eastern edges of Tibetan Plateau and upper reaches of the Yangtze River. The paper mainly identified the short-term influences of experimental warming, nitrogen fertilization, and their combination on growth and physiological performances of Picea asperata seedlings. These seedlings were subjected to two levels of temperature (ambient; infrared heater warming) and two nitrogen levels (0; 25 g m -2 a -1 N) for 6 months. We used a free air temperature increase of overhead infrared heater to raise both air and soil temperature by 2.1 and 2.6°C, respectively. The temperature increment induced an obvious enhancement in biomass accumulation and the maximum net photosynthetic rate, and decreased AOS and MDA level under ambient nitrogen conditions. Whereas, negative effects of experimental warming on growth and physiology was observed under nitrogen fertilization condition. On the other hand, nitrogen fertilization significantly improved plant growth in unwarmed plots, by stimulating total biomass, maximum net photosynthetic rate (A max ), antioxidant compounds, as well as reducing the content of AOS and MDA. However, in warmed plots, nitrogen addition clearly decreased A max , antioxidant compounds, and induced higher accumulation of AOS and MDA. Obviously, the beneficial effects of sole nitrogen on growth and physiology of Picea asperata seedlings could not be magnified by artificial warming.

show abstract

“…Proline accumulation is a very common response in plants exposed to drought stress (Yancey et al 1982;Alexieva et al 2001). It could function as a hydroxyl radical scavenger to prevent membrane damage and protein denaturation (Ain-Lhout et al 2001). POD and CAT are important enzymes in plants that protect plants against oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

Effects of drought stress and selenium supply on growth and physiological characteristics of wheat seedlings

2009

View full text Add to dashboard Cite

The paper studied the effects of drought stress, selenium (Se) supply and their combination on growth and physiological characteristics of wheat (Triticum aestivum L. cv Shijiazhuang NO. 8) seedlings. The experimental design included two water treatments (well-watered, 75% of maximum field capacity; drought stress, 30% of maximum field capacity) and two Se levels (0; 0.5 mg/kg) to determine whether Se can modify the negative impacts of drought stress on seedling growth and physiological traits. Drought stress caused a marked decline in growth parameters and soluble protein content, whereas it induced an increase in root activity, proline content and the activities of peroxidase (POD) and catalase (CAT) of leaf tissue. On the other hand, Se supply induced an increase in biomass accumulation only under wellwatered condition. Under drought stress, Se supply increased free proline content, root activity and the activities of POD and CAT in leaf tissue, but did not significantly affect on growth parameters. These results implied that drought stress brought harmful effects on wheat seedlings, and that Se supply was favorable for biomass accumulation of wheat seedlings under well-watered condition. However, it did not significantly affect on biomass accumulation under drought stress, although it increased root activity and activities of some antioxidant index in experimental periods.

show abstract

Untitled

Cited by 123 publications

References 37 publications

The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

Growth and physiological responses of Picea asperata seedlings to elevated temperature and to nitrogen fertilization

Effects of drought stress and selenium supply on growth and physiological characteristics of wheat seedlings

Contact Info

Product

Resources

About