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Groves, Michael J.; Christopherson, T; Giometto, Bruno; Salvatore, Francesco

doi:10.1023/a:1018541726460

Cited by 92 publications

(8 citation statements)

References 45 publications

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“…In subtypes of sensory neurons after injury, we identified unique expression of cell survival and death related genes28. For example, we found that neuronal cell death gene Caspase-3 in blue module is up-regulated in all three types of DRG neurons (Fig.…”

Section: Resultsmentioning

confidence: 90%

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Huang

et al. 2016

Sci Rep

133

120

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Peripheral nerve injury leads to various injury-induced responses in sensory neurons including physiological pain, neuronal cell death, and nerve regeneration. In this study, we performed single-cell RNA-sequencing (scRNA-seq) analysis of mouse nonpeptidergic nociceptors (NP), peptidergic nociceptors (PEP), and large myelinated sensory neurons (LM) under both control and injury conditions at 3 days after sciatic nerve transection (SNT). After performing principle component and weighted gene co-expression network analysis, we categorized dorsal root ganglion (DRG) neurons into different subtypes and discovered co-regulated injury-response genes including novel regeneration associated genes (RAGs) in association with neuronal development, protein translation and cytoplasm transportation. In addition, we found significant up-regulation of the genes associated with cell death such as Pdcd2 in a subset of NP neurons after axotomy, implicating their actions in neuronal cell death upon nerve injury. Our study revealed the distinctive and sustained heterogeneity of transcriptomic responses to injury at single neuron level, implicating the involvement of different gene regulatory networks in nerve regeneration, neuronal cell death and neuropathy in different population of DRG neurons.

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Section: Resultsmentioning

confidence: 90%

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Huang

et al. 2016

Sci Rep

133

120

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“…Axotomy of sensory nerves at adulthood often leads to the death of their ganglion cells (Aldskogius and Arvidsson, 1978; Aldskogius et al, 1985; Arvidsson and Grant, 1979; Groves et al, 1997; McKay Hart et al, 2002; Morest et al, 1998; Persson et al, 1991; Spoendlin, 1984; Westrum et al, 1976; Zuniga, 1999). A similar loss of geniculate ganglion cells following CTX may account, in part, for the reduced labeled terminal field volume that we show here.…”

Section: Resultsmentioning

confidence: 99%

Impact of chorda tympani nerve injury on cell survival, axon maintenance, and morphology of the chorda tympani nerve terminal field in the nucleus of the solitary tract

Reddaway

Davidow

Deal

et al. 2012

J of Comparative Neurology

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Chorda tympani nerve transection (CTX) has been useful to study the relationship between nerve and taste buds in fungiform papillae. This work demonstrated that the morphological integrity of taste buds depends on their innervation. Considerable research focused on the effects of CTX on peripheral gustatory structures, but much less research has focused on the central effects. Here, we explored how CTX affects ganglion cell survival, maintenance of injured peripheral axons, and the chorda tympani nerve terminal field organization in the nucleus of the solitary tract (NTS). After CTX in adult rats, the chorda tympani nerve was labeled with biotinylated dextran amine at 3, 7, 14, 30, and 60 days post-CTX to allow visualization of the terminal field associated with peripheral processes. There was a significant and persistent reduction of the labeled chorda tympani nerve terminal field volume and density in the NTS following CTX. Compared with controls, the volume of the labeled terminal field was not altered at 3 or 7 days post-CTX; however, it was significantly reduced by 44% and by 63% at 30 and 60 days post-CTX, respectively. Changes in the density of labeled terminal field in the NTS paralleled the terminal field volume results. The dramatic decrease in labeled terminal field size post-CTX cannot be explained by a loss of geniculate ganglion neurons or degeneration of central axons. Instead, the function and/or maintenance of the peripheral axonal process appear to be affected. These new results have implications for long-term functional and behavioral alterations.

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“…Not only motor neurons and DRG cells but also small neurons, presumably interneurons in the spinal cord, may undergo neuronal death as a result of neonatal peripheral nerve transection [28]. The neurodegeneration in DRG may result from the migration of mononuclear cells or apoptosis [29]. After serious craniocervical trauma, severely damaged DRG neurons were observed [25].…”

Section: Discussionmentioning

confidence: 99%

“…Stereology is a method to relate three-dimensional parameters of a structure from two-dimensional measurements obtained in the sections [31–36]. A detailed description of our dissector method was made [12–14, 29]. …”

Section: Discussionmentioning

confidence: 99%

Experimental research Effects of ischemic phrenic nerve root ganglion injury on respiratory disturbances in subarachnoid hemorrhage: an experimental study

Ulvi¹,

Demir²,

Aygül³

et al. 2013

aoms

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IntroductionPhrenic nerves have important roles on the management of respiration rhythm. Diaphragm paralysis is possible in phrenic nerve roots ischemia in subarachnoid hemorrhage (SAH). We examined whether there is a relationship between phrenic nerve root ischemia and respiratory disturbances in SAH.Material and methodsThis study was conducted on 5 healthy control and 14 rabbits with experimentally induced SAH by injecting autologous blood into their cisterna magna. Animals were followed up via monitors for detecting the heart and respiration rhythms for 20 days and then decapitaed by humanely. Normal and degenerated neuron densities of phrenic nerve root at the level of C4 dorsal root ganglia (C4DRG) were estimated by Stereological methods. Between the mean numerical density of degenerated neurons of C4DRG and respiratory rate/minute of groups were compared statistically.ResultsPhrenic nerve roots, artery and diaphragm muscles degeneration was detected in respiratory arrest developed animals. The mean neuronal density of C4DRG was 13272 ±1201/mm3 with a mean respiration rate of 23 ±4/min in the control group. The mean degenerated neuron density was 2.240 ±450/mm3 and respiration rhythm was 31 ±6/min in survivors. But, the mean degenerated neuron density was 5850 ±650/mm3 and mean respiration rhythm was 34 ±7/min in respiratory arrest developed animals (n = 7). A linear relationship was noticed between the degenerated neuron density of C4DRG and respiraton rate (r = –0.758; p < 0.001).ConclusionsPhrenic nerve root ischemia may be an important factor in respiration rhythms deteriorations in SAH which has not been mentioned in the literature.

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Cited by 92 publications

References 45 publications

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Impact of chorda tympani nerve injury on cell survival, axon maintenance, and morphology of the chorda tympani nerve terminal field in the nucleus of the solitary tract

Experimental research Effects of ischemic phrenic nerve root ganglion injury on respiratory disturbances in subarachnoid hemorrhage: an experimental study

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