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Abstract

We have previously isolated a phosphate starvation-response (psr) cDNA clone, psr3.1, from Brassica nigra which encodes a beta-glucosidase. Southern blots of Arabidopsis thaliana genomic DNA probed with the psr3.1 cDNA indicated that this gene exists as a single locus. A genomic library of A. thaliana was screened at high stringency to isolate the corresponding genomic clone. The resultant clone was coined psr3.2 because of its sequence divergence from isolated psr3.1 cDNA clones. Northern blotting with probes derived from the coding region of the genomic clone showed that this gene is expressed at high levels in P(i)-starved roots and the enhancement occurred within two days of growth in medium lacking P(i). The expression of this gene is repressed by heat shock and anaerobic conditions, and it is not significantly induced by high salinity, or by nitrogen or sulfur deprivation. Sequence analysis of the genomic clone revealed the existence of 13 exons interrupted by 12 AT-rich introns and it possessed a high homology with the B. nigra psr3.1 as well as various other beta-glucosidase genes from other species. Sequence similarity and divergence percentages between the deduced amino acid sequences of the psr3 clones and other beta-glycosidases suggests that they should be included along with two other Brassicaceae genes in a distinct subfamily of the BGA glycosidase gene family. The presence of an endoplasmic reticulum retention signal at the carboxy terminus indicates the likely cellular location of PSR3.2. The possible metabolic and regulatory roles of this enzyme during the P(i)-starvation response are discussed.

The Trichoderma harzianum Kelch Protein ThKEL1 Plays a Key Role in Root Colonization and the Induction of Systemic Defense in Brassicaceae Plants

“…Furthermore, this effect was enhanced under abiotic stress conditions, which could be expected considering the higher tolerance to salt and osmotic stresses previously reported in AtKel plants (Hermosa et al, 2011). The increase in seed yields may be related to the ability of those proteins with Kelch-repeat domains to improve the number and size of seeds, as previously described in rice (Chen et al, 2013), to the ratio of β-glucosidases in the nutritional cycle (Caruso, 2010), or to the inorganic phosphate accumulation in plants as reported in Arabidopsis (Malboobi and Lefebvre, 1997).…”

Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization

“…In general, the functions and activity of β-glucosidases depend on the substrate specificity, tissue localization, cellular localization, and substrate interactions. In plants, β-glucosidases are involved in various physiological processes in plants, such as cell wall lignification, hormone metabolism in plants, and plant defense responses to adverse conditions [56][57][58][59][60].…”

Functional Assessment of an Overexpressed Arabidopsis Purple Acid Phosphatase Gene (Atpap26) in Tobacco Plants

“…Arabidopsis thaliana ecotype Col-0 plants were cultivated hydroponically following the procedure described previously ( 24 ). Twenty-eight days old A. thaliana plant roots were used for RNA extraction and AtPAP26 cDNA cloning as described ( 25 ).…”