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Zhong, Jian‐Jiang; Meng, Xian-Dan; Zhang, Yi-Heng; Liu, Song

doi:10.1023/a:1018434420890

Cited by 9 publications

(3 citation statements)

References 9 publications

(5 reference statements)

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“…Cell permeability plays an important role in substance exchange between the cell and its surrounding medium [30]. Several methods have been developed to artificially overcome the bottleneck presented by cell permeability, either partially or completely, to release massive amounts of target products into the medium, such as permeabilization by organic solvents [30, 31]. Zhong et al [31] demonstrated that dimethyl sulfoxide (DMSO) treatment increases the permeability of Panax notoginseng cells.…”

Section: Introductionmentioning

confidence: 99%

“…Several methods have been developed to artificially overcome the bottleneck presented by cell permeability, either partially or completely, to release massive amounts of target products into the medium, such as permeabilization by organic solvents [30, 31]. Zhong et al [31] demonstrated that dimethyl sulfoxide (DMSO) treatment increases the permeability of Panax notoginseng cells. Takeshige et al [32] illustrated the use of toluene to permeabilize Saccharomyces cerevisiae cells.…”

Section: Introductionmentioning

confidence: 99%

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N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei

Chen

Shen

et al. 2019

Biotechnol Biofuels

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Background The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N , N -dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries. Results In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the T. reesei hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, we determined that plc - e encoding phospholipase C was activated by DMF and revealed that cytosolic Ca 2+ plays a vital role in the response of T. reesei to DMF. Using EGTA (a putative extracellular Ca 2+ chelator) and LaCl 3 (a plasma membrane Ca 2+ channel blocker), we demonstrated that DMF induced a cytosolic Ca 2+ burst via extracellular Ca 2+ and Ca 2+ channels in T. reesei , and that the cytosolic Ca 2+ burst induced by DMF-mediated overexpression of cellulase through calcium signaling. Deletion of crz1 confirmed that calcium signaling plays a dominant role in DMF-induced cellulase production. Additionally, 0.5–2% DMF increases the permeability of T. reesei mycelia for cellulase release. Simultaneous supplementation with 1% DMF and 10 mM Mn 2+ to T. reesei Rut-C30 increased cellulase activity approximately fourfold compared to that without treatment and was also more than that observed in response to either treatment alone. Conclusions Our results reveal that DMF-induced cellulase production via calcium signaling and permeabilization. Our results also provide insight into the role of calcium signaling in enzyme production for enhanced cellulase production and the development of novel inducers of cellulase. Electronic supplementary material The online version of this article (10.1186/s13068-019-1375-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei

Chen

Shen

et al. 2019

Biotechnol Biofuels

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“…The activated carbon is prepared by physical processing or chemical methods with the carbon-containing materials as the mainly raw materials [2]. The vast majority of the carbon-containing materials can be used in preparing activated carbon, such as the sawdust, the nutshell, the waste plastics and other waste [3].…”

Section: Introductionmentioning

confidence: 99%

Study on the Preparation of Activated Carbon from Corncobs

Yang²,

Sun

et al. 2013

AMR

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The impact of activator varieties on the activation effect in preparing activated carbon with corncob adopting chemical activation process were researched in this paper, the results showed that phosphoric acid as the activator was much better than potassium hydroxide and zinc chloride. It was deduced from the orthogonal experiment results that the impact of activation temperature on the activation effect is the greatest, impregnation ratio takes the second place, and the activator concentration the least. Suitable parameters of activation process were obtained: when the activator concentration is 50%, activation temperature 500°C, impregnation ratio 2.7:1, the iodine value of activated carbon is 822.08mg/g.

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Downstream Processing of Red Beet Hairy Roots

Neelwarne

Rudrappa²

2012

Red Beet Biotechnology

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Untitled

Cited by 9 publications

References 9 publications

N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei

N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei

Study on the Preparation of Activated Carbon from Corncobs

Downstream Processing of Red Beet Hairy Roots

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