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Nagano, Kasuke; Nakayama, Eiki; Koyano, Michiko; Oobayashi, Hisao; Adachi, Haruo; Yamada, Tohru

doi:10.1539/joh1959.21.29

Cited by 73 publications

(12 citation statements)

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“…Exposure to EGME induces a broad spectrum of embryotoxic effects (Nelson et al, 1989). In addition, testicular damage and dysfunction were observed in adult animals (Foster et al, 1984;Nagano et al, 1979). However, MAA elicited no specific effects in the tests addressing endocrine disruptors (EDs) (see Table 1 in (Schenk et al, 2010), and no specific assay for male fertility was included (Schenk et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test

et al. 2015

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-The embryonic stem cell test (EST) is a promising system to detect embryotoxicity in vitro. Recent studies have pointed out some limitations of the EST and suggest that the applicability domain of the EST and its prediction model have to be better defined. Here, eight substances of known reproductive toxicity were tested in the EST under blind conditions. We applied the prediction model to the data of the EST after classifying the substances according to the published criteria. In addition, a simplified classification of the EST results into two classes as an approach to hazard assessment was compared to the European Union Classification, Labelling and Packaging (CLP) Regulation labels of the substances. With one exception, substances that are labeled as reproductive toxicants according to the CLP Regulation were detected as embryotoxic in the EST while substances without label were found to be non-embryotoxic according to the EST.

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Section: Resultsmentioning

confidence: 99%

Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test

et al. 2015

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“…On the other hand, EGME has been reported to induce testicular toxicity in several mammalian species such as mice (Nagano et al, 1979), rats (Foster et al, 1983), hamsters (Nagano et al, 1984), guinea pigs (Nagano et al, 1979), rabbits (Miller et al, 1983), and humans (Johanson, 2000). The primary target of EGME is pachytene spermatocytes because the testicular lesions are initially restricted to the primary spermatocytes undergoing post zygotene meiotic maturation and division (Foster et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA profling in ethylene glycol monomethyl ether-induced monkey testicular toxicity model

et al. 2015

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-To establish and characterize ethylene glycol monomethyl ether (EGME)-induced testicular toxicity model in cynomolgus monkeys, EGME at 0 or 300 mg/kg was administered orally to sexually mature male cynomolgus monkeys (n = 3/group) for 4 consecutive days. Circulating and testicular micro-RNA (miRNA) profiles in this model were investigated using miRNA microarray or real-time quantitative reverse transcription-PCR methods. EGME at 300 mg/kg induced testicular toxicity in all the monkeys, which was characterized histopathologically by decreases in pachytene spermatocytes and round spermatids, without any severe changes in general conditions or clinical pathology. In microarray analysis, 16 down-regulated and 347 up-regulated miRNAs were detected in the testis, and 326 down-regulated but no up-regulated miRNAs were detected in plasma. Interestingly, miR-1228 and miR-2861 were identified as abundant miRNAs in plasma and the testis of control animals, associated presumably with apoptosis and cell differentiation, respectively, and were prominently increased in the testis of EGME-treated animals, reflecting the recovery from EGME-induced testicular damages via stimulating cell proliferation and differentiation of sperm. Furthermore, down-regulation of miR-34b-5p and miR-449a, which are enriched in meiotic cells like pachytene spermatocytes, was obvious in the testis, suggesting that these spermatogenic cells were damaged by the EGME treatment. In conclusion, EGME-induced testicular toxicity in cynomolgus monkeys was shown, and this model would be useful for investigating the mechanism of EGME-induced testicular toxicity and identifying testicular biomarkers. Additionally, testicular miR-34b-5p and miR-449a were suggested to be involved in damage of pachytene spermatocytes.

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“…Oral doses of 250 mg/kg or more, administered 5 times weekly for 5 weeks, produced such lesions in male mice [26]. Histological examination revealed degeneration of the seminiferous epithelium.…”

Section: Effects On the Testesmentioning

confidence: 97%

“…When male mice were given oral doses of 2-methoxyethanol on 5 days per week for 5 weeks, leukopenia developed at doses of 500 mg/kg and day or more and, at doses of 1000 mg/kg and day or more, also reductions in erythrocyte count and blood haemoglobin levels [26].…”

Section: Effects On the Bloodmentioning

confidence: 99%

2‐Methoxyethanol [MAK Value Documentation, 1993]

2012

The MAK‐Collection for Occupational Health and Safety

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Published in the series Occupational Toxicants , Vol. 6 (1993) The article contains sections titled: Toxic Effects and Modes of Action Effects in Man Effects on Animals Acute toxicity Effects on the central nervous system Effects on the blood Effects on the testes Teratogenic effects Genotoxicity and Cytotoxicity Manifesto (MAK value, classification)

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Cited by 73 publications

References 0 publications

Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test

Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test

MicroRNA profling in ethylene glycol monomethyl ether-induced monkey testicular toxicity model

2‐Methoxyethanol [MAK Value Documentation, 1993]

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