Untitled

Veld, Willem A. Man in ‘t; Cock, A. W. A. M. de; Ilieva, E.; Lévesque, C. André

doi:10.1023/a:1013929110325

Cited by 40 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By taking advantage of the phylogenetic analyses, many new species have been separated from some well known species complexes. For instance, P. brassicae (Man in 't Veld et al 2002) was separated from P. porri. P. obscura (Grü enwald et al 2012) was separated from P. syringae.…”

Section: Introductionmentioning

confidence: 99%

A high-temperature tolerant species in clade 9 of the genus Phytophthora: P. hydrogena sp. nov.

2014

View full text Add to dashboard Cite

A previously unknown Phytophthora species was isolated from irrigation water in Virginia, USA. This novel species produces abundant noncaducous and nonpapillate sporangia in soil water extract solution. It sometimes produces chlamydospores and hyphal swellings in aged cultures and in Petri's solution. This species has optimum vegetative growth at 30 C and grows well at 35 C. The lowest and highest temperatures for growth are 5 and 40 C. All isolates examined in this study are compatibility type A1 and produce mostly plerotic oospores when paired with an A2 mating-type tester of P. cinnamomi. Sequence analyses of the rDNA internal transcribed spacer (ITS) regions and the mitochondrially encoded cytochrome c oxidase 1 (cox 1) gene placed this species in clade 9 of the genus Phytophthora. These characteristics support the description of this taxon as a new species for which we propose the name P. hydrogena sp. nov. Further phylogenetic and physiological investigations of clade 9 species revealed a high-temperature tolerant cluster including P. hydrogena, P. aquimorbida, P. hydropathica, P. irrigata, P. chrysanthemi, P. insolita, P. polonica and P. parsiana. These species all grow well at 35 C. The monophyly of the species in this heat-tolerant cluster except P. insolita and P. polonica is highly supported by the maximum-likelihood analyses of the ITS and cox 1 sequences.

show abstract

Section: Introductionmentioning

confidence: 99%

A high-temperature tolerant species in clade 9 of the genus Phytophthora: P. hydrogena sp. nov.

2014

View full text Add to dashboard Cite

show abstract

Phytophthora brassicaeas a Pathogen of Arabidopsis

Mauch

Torche

Schläppi

et al. 2008

Oomycete Genetics and Genomics

View full text Add to dashboard Cite

High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

et al. 2019

View full text Add to dashboard Cite

The Canadian beekeeping industry is spread across the country, with the greatest proportion of managed honey bee colonies occurring in the Prairie Provinces. Nationally, the number of beekeepers has recently been trending upwards. Simultaneously, agronomic and environmental plant pest incidents are increasing due to a number of factors, including the introduction of exotic organisms through international trade, which is a major pathway for the introduction of potentially invasive alien species and quarantine pests. Therefore, regulatory agencies are interested in developing high‐throughput tools to achieve earlier detection of unwanted species in order to expedite application of mitigating measures to limit the impacts of their introduction. This study evaluates the potential of pollen pellet contents collected by honey bees to monitor plant pests using metabarcoding, a high‐throughput sequencing (HTS) approach for monitoring complex environmental samples. The study used the ITS1 intergenic region to target oomycetes and fungi, the ATP9‐NAD9 spacer to specifically target Phytophthora species, and the ITS2 region to target plant species. From the HTS results, a number of plants that were detected corresponded to known hosts of certain pathogens or species closely related to potentially invasive plant species. Genera including phytopathogenic species found in the pollen samples comprised Fusarium sp., Ophiostoma sp., Peronospora sp., Phytophthora sp., and Pythium sp. Correlations, high entropy, and co‐occurrences between certain plants and oomycetes or fungi were observed. The potential for using honey bee‐collected pollen pellets to study phytopathogens in a given environment is demonstrated here, and this concept could represent a promising complementary tool for the surveillance of phytopathogens or unwanted plants with previously described air and insect sampling methods if the protocol was applied with additional genetic markers.

show abstract

Untitled

Cited by 40 publications

References 21 publications

A high-temperature tolerant species in clade 9 of the genus Phytophthora: P. hydrogena sp. nov.

A high-temperature tolerant species in clade 9 of the genus Phytophthora: P. hydrogena sp. nov.

Phytophthora brassicaeas a Pathogen of Arabidopsis

High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

Contact Info

Product

Resources

About