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Paponov, V. D.; Paponov, V. V.; Байдакова, Г. В.

doi:10.1023/a:1015866129473

Cited by 1 publication

(2 citation statements)

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“…BPEp is highly expressed during the later stages of petal development, while ARF8 is ubiquitously expressed, but more highly expressed during the later stages of petal development. The expression of both FRL1 and ARF8 is increased in response to auxin [23,24,35], and as auxin responses are reduced in KSK over-expressing lines ( Figure 6E), it is possible that KSK over-expression may reduce expression of these auxin-responsive negative regulators of petal cell size, leading to increased petal cell size and overall increases in petal area. The down-regulation of ANT expression in KSK over-expression lines ( Figure 4C) is consistent with reduced auxin responses, as auxin increases ANT gene expression [36].…”

Section: Discussionmentioning

confidence: 99%

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Variation in the expression of a transmembrane protein influences cell growth inArabidopsis thalianapetals by altering auxin availability

Miller

Dumenil

Smith

et al. 2020

Preprint

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Background. The same species of plant can exhibit highly diverse sizes and shapes of organs that are genetically determined. Defining genetic variation underlying this morphological diversity is an important objective in evolutionary studies and it also helps identify the functions of genes influencing plant growth and development. Extensive screens of mutagenised Arabidopsis populations have identified multiple genes and mechanisms affecting organ size and shape, but relatively few studies have exploited the rich diversity of natural populations to identify genes involved in growth control. Results. We screened a relatively well characterised collection of Arabidopsis thaliana ecotypes for variation in petal size. Association analyses identified sequence and gene expression variation on chromosome 4 that made a substantial contribution to differences in petal area. Variation in expression of At4g16850 (named as KSK), encoding a hypothetical protein, had a substantial role on variation in organ size by influencing cell size. Over-expression of KSK led to larger petals with larger cells and promoted the formation of stamenoid features. The expression of auxin-responsive genes known to limit cell growth was reduced in response to KSK over-expression. ANT expression was also reduced in KSK over-expression lines, consistent with altered floral identities. Auxin availability was reduced in KSK over-expressing cells, consistent with changes in auxin-responsive gene expression. KSK may therefore influence auxin availability during petal development. Conclusions. Understanding how genetic variation influences plant growth is important for bothevolutionary and mechanistic studies. We used natural populations of Arabidopsis thaliana to identify sequence variation in a promoter region of Arabidopsis ecotypes that mediated differences in the expression of a previously uncharacterised membrane protein. This variation contributed to altered auxin availability and cell size during petal growth.

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Section: Discussionmentioning

confidence: 99%

“…One common feature of ARF8 and FRL1 expression, which was suppressed by over-expression of KSK, is that the expression of both genes increases in response to auxin [20,23,24]. We therefore tested auxin responses in petals of wt Col-0 and hrs to allow for protein expression, protoplasts were treated with either 0 nM or 1000 nM IAA.…”

Section: Figure 4bmentioning

confidence: 99%