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Abstract

ABSTRACT:A Mycobacterium DNA extraction protocol utilizing SPION developed and compared with the conventional and commercial kit method. The quality, quantity, and feasibility of the recovered DNA from all four different methods towards various molecular techniques were observed. In our work, morphology, bonding and composite of SPION is determined by various characterization techniques such as XRD, FT-IR, VSM, UV-vis spectroscopy and FE-SEM. XRD analysis confirms the crystallite size and phase purity of the synthesized material. The average crystallite size obtained were ~ 9.7 nm for SPION, prepared by a Co-precipitation method. FT-IR spectrum also confirms the formation of SPIONs. UV-vis spectrophotometry and gel electrophoresis analysis resulted in highest MTB DNA yield (985.3 ng/µl) and the purity was measured by the ratio A260 / A280 = 1.89. The comparative study suggested that the protocol developed in this report is a unique cost effective and highly pure. Further, Real-time PCR also confirms the superiority of our technique.