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Coletta-Filho, Helvécio Della; Machado, Marcos Antônio; Targon, M. L. P. N.; Moreira, Milene; PompeuJr., J.

doi:10.1023/a:1018300900275

Cited by 85 publications

(11 citation statements)

References 15 publications

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“…C. reshni was more distantly related and separated from the orange-mandarin group at the similarity value of 0.68. The observation that C. deliciosa was more closely related to C. reticulata and C. reshni was more distantly related was earlier shown by Filho et al (1998) also based on RAPD markers. This molecular characterization confirmed the earlier classification by Tanaka (1954) who recognized 36 species of mandarins in five taxonomic groups; C. reticulata and C. deliciosa were placed in Group III whereas C. reshni in Group IV.…”

Section: Resultssupporting

confidence: 61%

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Al-Anbari¹,

Kanawapee²,

AL-Kazragi³

et al. 2014

Afr. J. Agric. Res.

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Citrus is an economically important fruit crop with a long history of cultivation worldwide. A great number of varieties of citrus are extensively grown in the Middle East including Iraq for domestic consumption and exports. However, the genetic diversity of this genus in Iraq has not been reported. Therefore, the objective of this study was to evaluate genetic relationships of Iragi citrus genotypes to provide useful information for germplasm conservation and planning of breeding strategies. Twenty decamer primers were used to generate RAPD markers to evaluate genetic relationship among 16 genotypes (14 species and hybrids) of cultivated Citrus in Iraq. Based on RAPD polymorphisms, the citrus genotypes were classified into two main groups; the first consisted of citron (Citrus medica) and its hybrids (lime and lemon). The second group contained the remaining genotypes including three subgroups; the first being formed of sour orange (Citrus aurantium), sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi), the second the mandarins (Citrus reticulata) and the third the pummelo (Citrus grandis). The RAPD-based classification was consistent with previous studies based on other types of molecular markers.

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Section: Resultssupporting

confidence: 61%

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Al-Anbari¹,

Kanawapee²,

AL-Kazragi³

et al. 2014

Afr. J. Agric. Res.

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“…In this context, various Polymerase Chain Reaction (PCR) -based molecular marker tools such as Simple Sequence Repeats (SSRs), Amplified Fragment Length Polymorphism (AFLP), Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) have emerged as powerful tools for screening biodiversity. These techniques have been widely used to study the genetic diversity, taxonomy, cultivar identification (Fang et al, 1997;Filho et al, 1998;Novelli et al, 2000) and the construction of genetic linkage maps (Kijas et al, 1997;Sanker and Moore, 2001) in various Citrus spp. Of these markers, RAPD markers (Wiliams et al, 1990) that result from the PCR amplification of genomic DNA fragments using short oligonucleotides (usually 10-mers) of arbitrary sequence as primers have been widely used for diversity analyses as they are simple to use, cost effective and amplify multiple DNA loci through PCR (Wiliams et al, 1990;Abkenar and Isshiki, 2003;Baig et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

Munankarmi

Shrestha²,

Rana

et al. 2014

Int J Appl Sci Biotechnol

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Acid lime (Citrus aurantifolia Swingle) is an important commercial fruit crop, cultivated from terai to high hill landscapes of Nepal. However, production and productivity is very low due to various reasons including infestations by various diseases and pests, lack of diseases and pests resistant and high yielding varieties. In this context, determination of genetic variation at molecular level is fundamental to citrus breeders for the development of elite cultivars with desirable traits. In the present study, Random Amplified Polymorphic DNA (RAPD) marker technique has been employed to assess genetic diversity in 60 acid lime landraces representing different agro-ecological zones of eastern Nepal. Nine selected arbitrary primers generated 79 RAPD fragments of which 75 were polymorphic (94.94%). Phenogram was constructed by NTSYS-PC ver. 2.21i using UPGMA cluster analysis based on Jaccard's similarity coefficient to deduce overall genetic diversity and relationships of the acidlime genotypes under study. Sixty acid lime landraces formed seven clusters and similarity value ranged from 38% to 98% with an average of 72%. Genetic variation at different agro-ecological zones was assessed using Popgene ver. 1.32 and found 47% to 69.6% polymorphism. Shannon's index and Nei's gene diversity showed highest level of acid lime diversity in Terai zone (PPB, 69.62%; H, 0.213; I, 0.325) followed by mid-hill zone (PPB, 67.09%; H, 0.208; I, 0.317). The results obtained will be useful to citrus breeders for elite cultivar development. The RAPD-PCR technique is found to be the rapid and effective tool for genetic diversity assessment in acid lime landraces of Nepal.

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“…The main classification systems diverge with respect to the number of species included in this group from 36 species recognized by Tanaka (1969) to only three by Swingle and Reece (1967). Molecular studies also revealed the existence of a large heterogeneity among mandarins, but proposed that most accessions belong to a single species, Citrus reticulata Blanco (Luro et al 1995;Fang and Roose 1997;Coletta Filho et al 1998;Federici et al 1998;Nicolosi et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Chromosome homologies between Citrus and Poncirus—the comparative cytogenetic map of mandarin (Citrus reticulata)

Silva

Mendes

Filho

et al. 2014

Tree Genetics & Genomes

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Mandarins are among the main citrus species and constitute a morphologically diverse and taxonomically controversial group. Cytogenetic studies were limited to the determination of its karyotype formula and the localization of ribosomal DNA (rDNA) sites. The cytogenetic map of Poncirus trifoliata, established by fluorescent in situ hybridization (FISH) using single-copy bacterial artificial chromosomes (BACs) together with the localization of rDNA sequences and heterochromatin (CMA/DAPI banding pattern), represented an important milestone for the cytogenetic studies of the citrus species. In this work, the cytogenetic map of Citrus reticulata cv. Cravo, one of the pure species of the genus, was built using BACs from P. trifoliata, including the citrus tristeza virus resistance locus (Ctv), to detect homeologies and structural karyotypic changes between these species. Except for chromosomes 2 and 3, heterochromatin distribution and position of BACs were maintained in comparison to P. trifoliata, indicating a high synteny between both karyotypes and supporting a close phylogenetic relationship between these species. A break of colinearity was observed between BAC clones and the CMA + bands (including rDNA loci) from chromosomes 2 and 3, which could be mainly explained by amplification and reduction of the satellite DNA sequences that constitute the heterochromatic bands of these chromosome pairs.

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Cited by 85 publications

References 15 publications

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

Chromosome homologies between Citrus and Poncirus—the comparative cytogenetic map of mandarin (Citrus reticulata)

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