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Keiner, Ronald; Kaiser, Heike; Nakajima, Keiji; Hashimoto, Takashi; Dräger, Birgit

doi:10.1023/a:1013315110746

Cited by 44 publications

(10 citation statements)

References 24 publications

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“…For two features on the array, a maximum of 5 QTLs were identified. One shows strong homology to a tropinone reductase II (TRII; EST cSTB29M16TH ) [26] involved in the biosynthesis of tropane alkaloids which is known to be expressed in both below- and above-ground potato tissues. The second feature (BF_LBCHXXXX_0032H04_T3M.SCF) with 5 different QTLs has no significant homology to any protein present in the databases nor was its gene structure predicted in the current release of the potato genome annotation [25].…”

Section: Resultsmentioning

confidence: 99%

Organ specificity and transcriptional control of metabolic routes revealed by expression QTL profiling of source-sink tissues in a segregating potato population

et al. 2012

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BackgroundWith the completion of genome sequences belonging to some of the major crop plants, new challenges arise to utilize this data for crop improvement and increased food security. The field of genetical genomics has the potential to identify genes displaying heritable differential expression associated to important phenotypic traits. Here we describe the identification of expression QTLs (eQTLs) in two different potato tissues of a segregating potato population and query the potato genome sequence to differentiate between cis- and trans-acting eQTLs in relation to gene subfunctionalization.ResultsLeaf and tuber samples were analysed and screened for the presence of conserved and tissue dependent eQTLs. Expression QTLs present in both tissues are predominantly cis-acting whilst for tissue specific QTLs, the percentage of trans-acting QTLs increases. Tissue dependent eQTLs were assigned to functional classes and visualized in metabolic pathways. We identified a potential regulatory network on chromosome 10 involving genes crucial for maintaining circadian rhythms and controlling clock output genes. In addition, we show that the type of genetic material screened and sampling strategy applied, can have a high impact on the output of genetical genomics studies.ConclusionsIdentification of tissue dependent regulatory networks based on mapped differential expression not only gives us insight in tissue dependent gene subfunctionalization but brings new insights into key biological processes and delivers targets for future haplotyping and genetic marker development.

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Section: Resultsmentioning

confidence: 99%

Organ specificity and transcriptional control of metabolic routes revealed by expression QTL profiling of source-sink tissues in a segregating potato population

et al. 2012

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“…It has been reported that overexpression of the TRI gene can considerably enhance the production of tropane alkaloids in the root cultures of Atropa belladonna [4]. A TRII cDNA that does not accumulate the classical tropane alkaloids was also cloned from potato [9]. TRs are members of a short-chain dehydrogenase family with TR-homologous genes represented in plant species (e.g.…”

Section: Scheme 1 the Tropane-alkaloid Biosynthesis Pathwaymentioning

confidence: 99%

“…TRs are members of a short‐chain dehydrogenase family with TR‐homologous genes represented in plant species (e.g. Arabidopsis thaliana , Solanum tuberosum , Cochlearia officinalis ) that do not produce tropane alkaloids, suggesting that these enzymes may function in otherwise unrelated metabolic pathways [9,10].…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloid production in AaTRI‐transformed hairy roots

Kai¹,

Li²,

Jiang³

et al. 2009

Biotech and App Biochem

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Tropane alkaloids are used medicinally as anticholinergic agents with increasing market demand, so the improvement and production of active components from medicinal plants using molecular biotechnology show great potential for applications that should benefit human healthcare. Two tropinone reductases constitute a branching point in the biosynthesis of tropane alkaloids. In the present paper, we report for the first time the cloning and characterization of two fulllength cDNAs encoding TRI (tropinone reductase I) (GenBank accession number EU424321) and TRII (tropinone reductase II) (GenBank(R) accession number EU424322) from the solanaceous plant Anisodus acutangulus by rapid amplification of cDNA ends. Sequence comparison indicated that AaTRI (A. acutangulus TRI) and AaTRII (A. acutangulus TRII) had high homology with other tropinone reductases from Hyoscyamus niger, Datura stramonium etc., but AaTRI and AaTRII showed identity of only 60.8%. Phylogenetic-tree analysis showed that AaTRI and AaTRII belong to different clusters and have the closest relationship with H. niger TRI and TRII respectively. Expression-pattern analysis showed that AaTRI and AaTRII were expressed in all tissues tested, including root, stem and leaf, but the transcript level of AaTRI was much lower than AaTRII. Expression of AaTRI and AaTRII could be enhanced by methyl jasmonate, with a weak effect for AaTRI and a strong effect for AaTRII. AaTRI-transformed hairy-root lines were accompanied by a mean 1.87-fold higher level of hyoscyamine and a mean 8-fold higher level of scopolamine compared with control roots, indicating that AaTRI is a promising target for genetic engineering to increase tropane alkaloid in A. acutangulus.

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“…Tropinone is considered the key intermediate in the biosynthesis of tropane esters in Solanaceous plants. Reduction of tropinone results in the formation of either tropine or its stereoisomer pseudotropine, and is catalysed by tropinone reductase I, TRI (EC 1.1.1.206) and tropinone reductase II, TRII (EC 1.1.1.236), respectively [42][43][44]. The former is the precursor of most clinically used tropane esters, whereas the latter is an intermediate in the formation of some rarer esters (e.g.…”

Section: Nicotine Alkaloids Are Biosynthetically Linked To Tropane Almentioning

confidence: 99%

Tropane and Nicotine Alkaloid Biosynthesis-Novel Approaches Towards Biotechnological Production of Plant-Derived Pharmaceuticals

Oksman-Caldentey

2007

CPB

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Many plants belonging to the Solanaceae family have been used as a source of pharmaceuticals for centuries because of their active principles, tropane and nicotine alkaloids. Tropane alkaloids, atropine, hyoscyamine and scopolamine, are among the oldest drugs in medicine. On the other hand nicotine, the addictive agent in tobacco, has only recently gained attention as a backbone for novel potential alkaloids to be used for certain neurological diseases. The biotechnological production of alkaloids utilizing plant cells as hosts would be an attractive option. However, to date very little success in this field has been gained because of the lack of understanding how these compounds are synthesized in a plant cell. Metabolic engineering attempts have already shown that when the rate-limiting steps of the biosynthetic pathway are completely known and the respective genes cloned, the exact regulation towards desired medicinal products will be possible in the near future. The new functional genomics tools, which combine transcriptome and metabolome data, will create a platform to better understand a whole system and to engineer the complex plant biosynthetic pathways. With the help of this technology, it is not only possible to produce known plant metabolites more effectively but also to make arrays of new compounds in plants and cell cultures.

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Untitled

Cited by 44 publications

References 24 publications

Organ specificity and transcriptional control of metabolic routes revealed by expression QTL profiling of source-sink tissues in a segregating potato population

Organ specificity and transcriptional control of metabolic routes revealed by expression QTL profiling of source-sink tissues in a segregating potato population

Molecular cloning and characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloid production in AaTRI‐transformed hairy roots

Tropane and Nicotine Alkaloid Biosynthesis-Novel Approaches Towards Biotechnological Production of Plant-Derived Pharmaceuticals

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