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Potapovich, M. V.; Еремин, А. Н.; Metelitsa, D. I.

doi:10.1023/a:1022577611056

“…In assays where the exposure time was longer (from 6 to 10 min), the activity was lower and, in some cases, lower than the activity obtained in the enzyme extract without exposure to the ultrasonic probe, possibly due to the enzymatic denaturation caused by the longer exposure time to the ultrasonic probe (Kapturowska, Stolarzewicz and Krzyczkowska 2012). Denaturation of enzymes can occur due to excess pressure, temperature or even shear force generated during the cavitation phenomenon in the ultrasonic system (Grintsevich et al 2001;Potapovich, Eremin and Metelitza 2003).…”

Section: Ultrasonic Probementioning

confidence: 99%

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Czapela

¹

,

Kubeneck

²

,

Preczeski

³

et al. 2020

Preprint

1

0

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Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

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“…In assays where the exposure time was longer (from 6 to 10 min), the activity was lower and, in some cases, lower than the activity obtained in the enzyme extract without exposure to the ultrasonic probe, possibly due to the enzymatic denaturation caused by the longer exposure time to the ultrasonic probe (Kapturowska et al 2012). Denaturation of enzymes can occur due to excess pressure, temperature or even shear force generated during the cavitation phenomenon in the ultrasonic system (Grintsevich et al 2001;Potapovich et al 2003).…”

Section: Ultrasonic Probementioning

confidence: 99%

Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Czapela

¹

,

Kubeneck

²

,

Preczeski

³

et al. 2020

Bioresour. Bioprocess.

3

0

View full text Add to dashboard Cite

Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

show abstract

“…Denaturation of enzymes can occur due to excess pressure, temperature or even shear force generated during the cavitation phenomenon in the ultrasonic system (Grintsevich et al 2001;Potapovich, Eremin and Metelitza 2003).…”

Section: Ultrasonic Probementioning

confidence: 99%

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Czapela

¹

,

Kubeneck

²

,

Preczeski

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

show abstract

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Cited by 30 publications

References 10 publications

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

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