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Rojas-Beltran, J.; Dubois, Frédéric; Mortiaux, Frédéric; Portetelle, Daniel; Gebhardt, Christiane; Sangwan, Rajbir S.; Jardin, Patrick du

doi:10.1023/a:1006136624210

Cited by 24 publications

(5 citation statements)

References 28 publications

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“…Inorganic pyrophosphatase catalyzes the hydrolysis of pyrophosphate (PPi), which is formed principally as the product of the many biosynthetic reactions that utilize ATP. In the pulp, its function is probably related to regulation of inorganic pyrophosphate in the cytosol [65]. Fructose-bisphosphate aldolase is a glycolytic enzyme that catalyses the reversible aldol cleavage or condensation of fructose-1,6-bisphosphate into dihydroxyacetone-phosphate and glyceraldehyde 3-phosphate and is abundant in the pulp, but not the seeds of ripe berries [58].…”

Section: Discussionmentioning

confidence: 99%

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

et al. 2009

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In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well watered and water-deficit stress conditions. Of 1,047 proteins surveyed from pericarp by 2D-PAGE, 90 identified proteins showed differential expression between the skin and pulp. Of 695 proteins surveyed from seed tissue, 163 were identified and revealed that the seed and pericarp proteomes were nearly completely distinct from one another. Water-deficit stress altered the abundance of approximately 7% of pericarp proteins, but had little effect on seed protein expression. Comparison of protein and available mRNA expression patterns showed that 32% pericarp and 69% seed proteins exhibited similar quantitative expression patterns indicating that protein accumulation patterns are strongly influenced by post-transcriptional processes. About half of the 32 metabolites surveyed showed tissue-specific differences in abundance with water-deficit stress affecting the accumulation seven of these compounds. These results provide novel insights into the likely tissue-specific origins and the influence of water deficit stress on the accumulation of key flavor and aroma compounds in wine.

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Section: Discussionmentioning

confidence: 99%

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

et al. 2009

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“…This reaction generates inorganic pyrophosphate (PPi) as by-product. In plants, PPi plays a central role not only as by-product of activation and polymerization steps (Sonnewald, 1992; Geigenberger et al, 1998; Rojas-Beltran et al, 1999; Farre et al, 2001), if not as an energy donor per se (Stitt, 1998; Lopez-Marques et al, 2004). PPi is generally removed by inorganic pyrophosphatases, which hydrolyze PPi to orthophosphate (Pi).…”

Section: Introductionmentioning

confidence: 99%

Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit

et al. 2013

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Ascorbate (vitamin C) deficiency leads to low immunity, scurvy, and other human diseases and is therefore a global health problem. Given that plants are major ascorbate sources for humans, biofortification of this vitamin in our foodstuffs is of considerable importance. Ascorbate is synthetized by a number of alternative pathways: (i) from the glycolytic intermediates D-glucose-6P (the key intermediates are GDP-D-mannose and L-galactose), (ii) from the breakdown of the cell wall polymer pectin which uses the methyl ester of D-galacturonic acid as precursor, and (iii) from myo-inositol as precursor via myo-inositol oxygenase. We report here the engineering of fruit-specific overexpression of a bacterial pyrophosphatase, which hydrolyzes the inorganic pyrophosphate (PPi) to orthophosphate (Pi). This strategy resulted in increased vitamin C levels up to 2.5-fold in ripe fruit as well as increasing in the major sugars, sucrose, and glucose, yet decreasing the level of starch. When considered together, these finding indicate an intimate linkage between ascorbate and sugar biosynthesis in plants. Moreover, the combined data reveal the importance of PPi metabolism in tomato fruit metabolism and development.

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“…The first plant PPase gene identified was from Arabidopsis [5] and many cDNAs have since been found from several other plant species that are highly similar to this original sequence [6–9]. Immuno‐gold labelling using an antibody that recognizes the protein encoded by a potato cDNA demonstrated that it is localized in the cytosol [7], and it is reasonable to assume that all these similar genes code for cytoplasmic isoforms. In this paper, we report on an Arabidopsis gene revealed by the genome sequencing project coding for a PPase that is clearly different from all other higher plant PPases so far identified.…”

Section: Introductionmentioning

confidence: 99%

Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts

Schulze

Mant²,

Koßmann

et al. 2004

FEBS Letters

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An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl 2 was necessary for the protein's activity, whilst NaF inhibited it. The K m for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloro-plasts and localized to the stromal compartment.

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Cited by 24 publications

References 28 publications

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit

Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts

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