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Zvenigorodskiĭ, V I; Tyaglov, B. V.; Воейкова, Т. А.

doi:10.1023/a:1010229117963

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…For example, it was shown that the optimal additional carbon source for S. virginiae ATCC 13161 was d -maltose, which doubled virginiamycin production (Zvenigorodskii et al 2001). In the same study, good results were also obtained for mannose and d -glucose (yield increase by 50% of the control), but the use of complex carbohydrates, such as starch resulted in a lower effect.…”

Section: Resultsmentioning

confidence: 99%

“…The majority of such publications are devoted to the optimization of the medium components, including carbon/nitrogen sources and minor elements (Zvenigorodskii et al 2001; Zhao et al 2011; Yong 2015); some authors also describe the effect of addition of precursors (Han et al 2013) and regulators of the virginiamycin biosynthesis (Yang et al 1995). The optimization of fermentation conditions is described only in several studies.…”

Section: Discussionmentioning

confidence: 99%

“…An indirect evidence of this fact is a very small number of publications on the strain improvement of S. virginiae . The productivity of strains reported in various publications is relatively low and varies from 0.28 to 1.9 g/L (Biot 1984; Prikrylova et al 1987; Zvenigorodskii et al 2001; Zhao et al 2011). An additional increase of the strain productivity can be achieved by medium improvement and optimization of fermentation conditions, but even in this case the productivity of the existing industrial strains remains mainly within the range 3–4 g/L (Biot 1984; Zhao et al 2011; Han et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

Dzhavakhiya¹,

Savushkin²,

Ovchinnikov³

et al. 2016

3 Biotech

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Virginiamycin produced by Streptomyces virginiae as a natural mix of macrocyclic peptidolactones M and S is widely used in the industrial production of ethanol fuel and as an antibiotic feed additive for cattle and poultry. Its main antimicrobial components, M1 and S1 factors, act synergistically if the M1:S1 ratio in the final product is 70–75:25–30. This fact significantly complicates the development of stable high-yield strains suitable for industrial application. In the previous work, authors obtained a mutant S. virginiae VKM Ac-2738D strain, characterized by a high productivity in flasks and the optimum M1:S1 ratio (75:25) in the final product. In this study, the scale-up of the virginiamycin production by VKM AC-2738D from shake flasks to a pilot-scale (100 L) stirred fermentor was carried out and the possibility of the in situ use of synthetic adsorbing resins to remove virginiamycin from culture broth was assessed. After the optimization of pH and dissolved oxygen concentration (6.8–7.0 and 50%, respectively), the fed-batch fermentation of VKM Ac-2738D with continuous addition of 50% sucrose solution (5 g/L/day starting from 48 h of fermentation) resulted in a final virginiamycin titer of 4.9 g/L. Among four tested resins, Diaion® HP21 added to fermentation medium prior to sterilization absorbed 98.5% of the total virginiamycin that simplifies its further recovery procedure and increased its total titer to 5.6 g/L at the M1:S1 ratio of 74:26. The developed technology has several important advantages, which include (1) the optimum M1:S1 ratio in the final product, (2) the possibility to use sucrose as a carbon source instead of traditionally used and more expensive glucose or d-maltose, and (3) selective binding of up to 98.5% of produced virginiamycin on the adsorbing resin.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

Dzhavakhiya¹,

Savushkin²,

Ovchinnikov³

et al. 2016

3 Biotech

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Comparison of CID spectra of singly charged polypeptide antibiotic precursor ions obtained by positive‐ion vacuum MALDI IT/RTOF and TOF/RTOF, AP‐MALDI‐IT and ESI‐IT mass spectrometry

et al. 2006

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Various classes of polypeptide antibiotics, including blocked linear peptides (gramicidin D), side-chaincyclized peptides (bacitracin, viomycin, capreomycin), side-chain-cyclized depsipeptides (virginiamycin S), real cyclic peptides (tyrocidin, gramcidin S) and side-chain-cyclized lipopeptides (polymyxin B and E, amfomycin), were investigated by low-energy collision induced dissociation (LE-CID) as well as highenergy CID (HE-CID). Ion trap (IT) based instruments with different desorption/ionization techniques such as electrospray ionization (ESI), atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) and vacuum MALDI (vMALDI) as well as a vMALDI-time-of-flight (TOF)/curved fieldreflectron instrument fitted with a gas collision cell were used. For optimum comparability of data from different IT instruments, the CID conditions were standardized and only singly charged precursor ions were considered. Additionally, HE-CID data obtained from the TOF-based instrument were acquired and compared with LE-CID data from ITs. Major differences between trap-based and TOF-based CID data are that the latter data set lacks abundant additional loss of small neutrals (e.g. ammonia, water) but contains product ions down to the immonium-ion-type region, thereby allowing the detection of even single amino-acid (even unusual amino acids) substitutions. For several polypeptide antibiotics, mass spectrometric as well as tandem mass spectrometric data are shown and discussed for the first time, and some yet undescribed minor components are also reported. De novo sequencing of unusually linked minor components of (e.g. cyclic) polypeptides is practically impossible without knowledge of the exact structure and fragmentation behavior of the major components. Finally, the described standardized CID condition constitutes a basic prerequisite for creating a searchable, annotated MS n -database of bioactive compounds. The applied desorption/ionization techniques showed no significant influence on the type of product ions (neglecting relative abundances of product ions formed) observed, and therefore the type of analyzer connected with the CID process mainly determines the type of fragment ions.

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Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production

Tong

Zhou

Chen

et al. 2018

Bioprocess Biosyst Eng

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The production of virginiamycin (VGM) from Streptomyces virginiae was improved by genome shuffling and ribosome engineering companied with a high-throughput screening method integrating deep-well cultivation and the cylinder-plate detecting. First, a novel high-throughput method was developed to rapidly screen large numbers of VGM-producing mutants. Then, the starting population of genome shuffling was obtained through ultraviolet (UV) and microwave mutagenesis, and four mutants with higher productivity of VGM were selected for genome shuffling. Next, the parent protoplasts were inactivated by UV and heat when a fusant probability was about 98%. Streptomycin resistance was used as an evolutionary pressure to extend positive effects on VGM synthesis. Finally, after five rounds of genome shuffling, a genetically stable strain G5-103 was obtained and characterized to be able to yield 251 mg/L VGM, which was 3.1- and 11.6-fold higher than that of the mutant strain UV 1150 and the wild-type strain, respectively.

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Cited by 4 publications

References 3 publications

Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

Comparison of CID spectra of singly charged polypeptide antibiotic precursor ions obtained by positive‐ion vacuum MALDI IT/RTOF and TOF/RTOF, AP‐MALDI‐IT and ESI‐IT mass spectrometry

Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production

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