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Еремеев, Н. Л.; Belyaeva, E. A.; Bogdanovskaya, V. A.; Kazanskaya, N.F.

doi:10.1023/a:1006962228137

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Recently, stimuli-responsive polymers have been extensively applied to a variety of areas such as gene and drug delivery [1 -5], bioseparation [6], actuators [7], biosensors [8], artificial tissues [5,9,10], immunoassays [11,12] and others [6, 13 -15]. In particular, immunoassay performance was improved by adopting smart materials [11,12] such as poly(N -isopropyl acrylamide) [16,17], poly(methacrylic acid) [17] and poly(2-carboxyisopropylacrylamide) [18], which undergo physical or chemical changes in response to changes of pH or temperature of the system [19,20].…”

Section: Introductionmentioning

confidence: 99%

Polystyrene microbeads modified with an elastin-like biopolymer for stimuli-responsive immunodetection

Kim

Lee

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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An efficient and controlled method for immunodetection, using polystyrene (PS) microbeads conjugated with an elastin-like polypeptide (ELP), was investigated. ELP is a temperature-sensitive polymer that exhibits a hydrophilic-hydrophobic phase transition at the lower critical solution temperature. To introduce amine groups, ELP was modified with lysine for conjugation with PS microbeads functionalized with carboxylic groups. Prostate-specific antigen (PSA), a cancer marker, was detected from a biomolecular mixture using ELP-conjugated PS microbeads and ELP-conjugated antiPSA. External stimuli were used to reversibly separate the PSA-antiPSA complex. The stimuli-responsiveness of the ELP provided a designable generic biosurface for diagnostic detection.

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Section: Introductionmentioning

confidence: 99%

Polystyrene microbeads modified with an elastin-like biopolymer for stimuli-responsive immunodetection

Kim

Lee

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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“…This study demonstrates that the property of an enzymepolymer system is not determined by a mere sum of the properties of its components but rather can acquire new characteristics. The developed system is also applicable as a biosensor [21]. The protein-protein interaction has been studied by the example of an antibody-antigenenzyme system with the aim to develop a complex allowing the enzyme activity to be modulated by interaction of coupled proteins.…”

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confidence: 99%

10.1007/s10438-008-1001-7

2000

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Nanobiotechnology is defined as an interdisciplinary field of science that studies the application of fine-sized biological objects (of nanoscale, 1-100 nm) to design the devices and systems of the same size that utilize for new purposes the unusual, known, or previously unknown effects. Analysis demonstrates that the final goals, approaches, solution methods, and applications of nanostructures and biological sensors have much in common. This brief review attempts to systematize a number of the available data and pick out an organic connection of the new research direction with the field of biosensor technology, which have reached the level of sustainable development.

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Cited by 2 publications

References 13 publications

Polystyrene microbeads modified with an elastin-like biopolymer for stimuli-responsive immunodetection

Polystyrene microbeads modified with an elastin-like biopolymer for stimuli-responsive immunodetection

10.1007/s10438-008-1001-7

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