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Martínez, Lucía; Caballero‐Mellado, Jesús; Orozco, José; Martı́nez-Romero, Esperanza

doi:10.1023/a:1026283311770

Cited by 73 publications

(13 citation statements)

References 51 publications

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“…Klebsiella variicola comprises clinical as well as plant and insect associated bacteria (1–4). The species was described in 2004 mainly on the basis of a distinct phylogenetic position and low DNA-DNA hybridization to related species (4).…”

Section: Genome Announcementmentioning

confidence: 99%

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Draft Genome Sequences of Klebsiella variicola Plant Isolates

et al. 2015

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Three endophytic Klebsiella variicola isolates—T29A, 3, and 6A2, obtained from sugar cane stem, maize shoots, and banana leaves, respectively—were used for whole-genome sequencing. Here, we report the draft genome sequences of circular chromosomes and plasmids. The genomes contain plant colonization and cellulases genes. This study will help toward understanding the genomic basis of K. variicola interaction with plant hosts.

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Section: Genome Announcementmentioning

confidence: 99%

“…The endophytic isolates studied in this work were originally obtained from inside tissues from maize shoots (strain 3), banana leaves (strain 6A2), or sugar cane stem (strain T29A) (2, 4). Total genomic DNA from K. variicola isolates were extracted and purified using the DNeasy Kit (Qiagen, Germany).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Klebsiella variicola Plant Isolates

et al. 2015

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“…In the present study, no correlation was observed between the extent of colonization as indicated by CFU g −1 tissue and the number of isolates retrieved per sucker. Diverse endophytic bacterial species have been isolated from banana in the previous studies which include Pantoea agglomerans, Citrobacter sp., Klebsiella pneumoniae [24] [9]. A series of root associated bacterial endophytes were isolated from cv.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>

Sekhar¹,

Thomas²

2015

AJPS

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Endophytic bacteria colonizing the shoot-tips of banana cv. Grand Naine were isolated and tested for the antagonistic activity against the Panama wilt pathogen Fusarium oxysporum f. sp. cubense (Foc). Pre-isolation, the suckers were given extensive disinfection treatments and the homogenate from the excised shoot-tip portion was plated on nutrient agar (NA) and trypticase soy agar (TSA). This yielded altogether 47 isolates: 26 on NA and 21 on TSA, respectively, from the 10 suckers collected during August to February. The number of bacterial isolates obtained per sucker varied from one to 15 based on colony characteristics registering up to 10 distinct species per shoot-tip based on 16S rRNA sequence analysis. The 47 isolates belonged to 19 genera and 25 species under the phylogenetic classes of Actinobacteria, α-and γ-Proteobacteria and Firmicutes. Actinobacteria constituted the predominant phylum (55% isolates) with the constituent genera of Arthrobacter, Brevibacterium, Corynebacterium, Curtobacterium, Kocuria, Kytococcus, Micrococcus, Naumanella, Rothia and Tessaracoccus spp. and an unidentified isolate belonging to the family Frankiaceae. Proteobacteria constituted the second major phylum (Brevundimonas, Enterobacter, Klebsiella, Pseudomonas, Serratia and Sphingomonas spp.) followed by Firmicutes (Bacillus and Staphylococcus spp.). Antagonistic activity of the endophytes against Foc was tested through agar plate assays (pit and spot applications on fungal lawn) employing potato dextrose agar and NA. Endophytic Pseudomonas aeruginosa (isolate GNS.13.2a) which was associated with a single sucker showed significant growth inhibition effect on Foc while Klebsiella variicola (GNS.13.3a) and Enterobacter * Corresponding author.A. C. Sekhar, P. Thomas 944 cloacae (GNS13.4a) exhibited moderate inhibition. The study brings out considerable sucker to sucker variation in the associated cultivable endophytic bacteria in "Grand Naine" banana and identifies a few bacterial endophytes with biocontrol potential against the devastating Foc pathogen.

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“…It has also been found in association with other sucrose-rich crops, such as sweet potato, sweet sorghum, pineapple, banana and coffee (Reis et al 1994;Saravanan et al 2008;Martínez et al 2003;JimenezSalgado et al 1997). Several important biotechnological features related to plant growth promotion have been described for this Alphaproteobacterium.…”

Section: Introductionmentioning

confidence: 96%

Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media

2012

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The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. The recent genomic sequence of PAL5 unveiled the presence of a quorum sensing (QS) system. QS are regulatory mechanisms that, through the production of signal molecules or autoinducers, permit a microbial population the regulation of the physiology in a coordinated manner. The most studied autoinducers in gram-negative bacteria are the N-acyl homoserine lactones (AHLs). The usage of biosensor strains evidenced the presence of AHL-like molecules in cultures of G. diazotrophicus PAL5 grown in complex and synthetic media. Analysis of AHLs performed by LC-APCI-MS permitted the identification of eight different signal molecules, including C6-, C8-, C10-, C12- and C14-HSL. Mass spectra confirmed that this diazotrophic strain also synthesizes autoinducers with carbonyl substitutions in the acyl chain. No differences in the profile of AHLs could be determined under both culture conditions. However, although the level of short-chain AHLs was not affected, a decrease of 30% in the production of long-chain AHLs could be measured in synthetic medium.

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Cited by 73 publications

References 51 publications

Draft Genome Sequences of Klebsiella variicola Plant Isolates

Draft Genome Sequences of Klebsiella variicola Plant Isolates

Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>

Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media

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Untitled

Cited by 73 publications

References 51 publications

Draft Genome Sequences of Klebsiella variicola Plant Isolates

Draft Genome Sequences of Klebsiella variicola Plant Isolates

Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against &lt;i&gt;Fusarium oxysporum&lt;/i&gt; f. sp. &lt;i&gt;cubense&lt;/i&gt;

Identification of N-acyl homoserine lactones produced by Gluconacetobacter diazotrophicus PAL5 cultured in complex and synthetic media

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Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>