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Mirza, Muhammad Nadeem; Ahmad, Waseem; Latif, Farooq; Haurat, Jacqueline; Bally, René; Normand, Philippe; Malik, Kauser A.

doi:10.1023/a:1013388619231

Cited by 192 publications

(20 citation statements)

References 36 publications

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“…Nitrogenase activity was detected by acetylene reduction/ethylene production assay as described earlier (Mirza et al, 2001). Pure bacterial colonies were inoculated into NFM (Nitrogen Free Malate) semisolid medium vials and incubated at 28 ± 2 ∘ C for 48 h. Acetylene (10% v/v) was injected to the vials, incubated at 28 ± 2 ∘ C for 16 h and 100 μL of gas samples from the vials were analyzed on a gas chromatograph (Thermoquest, Trace G.C, Model K, Rodono Milan, Italy) equipped with a Porapak Q column and a H 2 -flame ionization detector (FID) using conditions described in Hardy et al (1973).…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion

et al. 2015

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The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion

et al. 2015

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“…Plant roots secrete signalling chemicals (L-Trp, a precursor for IAA, as well as other amino acids and small molecules) into the rhizosphere soil, promoting the binding of bacteria to the root surface (Simons et al, 1997). The root-bound bacteria may use the L-Trp in the soil to produce IAA (Patten & Glick, 1996;Mirza et al, 2001;Gravel et al, 2007). The IAA is subsequently secreted from the PGPR and absorbed by the plant and used primarily to increase cell growth or proliferation (Glick et al, 1998).…”

Section: Bacteria Strains and Growth Conditionsmentioning

confidence: 99%

Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Tabatabaei

Ehsanzadeh

Etesami

et al. 2016

Span. j. agric. res.

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The role of plant-associated bacteria in plant physiology and metabolism is well documented, but little has been known about the roles played by Pseudomonas in durum wheat (Triticum turgidum L. var durum) growth and development. An in vitroexperiment was conducted to observe the effect of the inoculation of four indole-3-acetic acid (IAA)-producing Pseudomonas isolates and exogenous IAA on seed germination traits and α-amylase activity of durum wheat. The results showed inoculation with all bacterial isolates led to a decrease in the germination percent, although the extent of the depression varied with the isolate. A significant relationship between concentrations of bacterial IAA and the germination inhibition percent in durum wheat seeds by different bacteria strains was observed. The results of this assay showed the effect of bacterial isolates on α-amylase activity after six and 8 days of inoculation was significant, while effect of these isolates on α-amylase activity after two and 4 days of inoculation was not meaningful. In addition, the exogenously applied IAA displayed a concentration-dependent effect on seed germination attributes and α-amylase activity, consistent with the possibility that the inhibitory effect of bacterial inoculation on seed germination was in consequence of bacteria-produced IAA. Therefore, it may suggested that the inhibitory role of IAA in seed germination and α-amylase activity should be taken into account during the screening of IAA-producing Pseudomonas isolates for durum wheat growth promoting agents.

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“…Besides, IAA stimulates seeds and tuber germination, increases the rate of xylem and root development, controls processes of vegetative growth; initiates lateral and adventitious root formation; mediates responses to light and gravity; affects photosynthesis, pigment formation, biosynthesis of various metabolites and resistance to stressful conditions (Gupta et al, 2015). The potential for IAA synthesis varies with different species and strains as well as cultural condition, growth stage and availability of substrate (Mirza et al, 2001). All 5 PSB were able to produce hydrogen cyanide, siderophores, ammonia and ACC deaminase.…”

Section: Screening Of Plant Growth Promoting Traitsmentioning

confidence: 99%

Screening for Lotus creticus growth promoting rhizobacteria under greenhouse conditions

Achkouk

Aarab

Laglaoui

et al. 2018

Eurasian Journal of Soil Science (Ejss)

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Utilization of plant growth promoting rhizobacteria (PGPR) is now gradually increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. This study was conducted with a view to isolate bacteria from the rhizosphere of the legume Lotus creticus (L. creticus) and to assess their plant growth promoting functional potentialities. A total of 113 rhizobacteria was isolated from the rhizosphere of L. creticus and were tested for their capacity of solubilizing tricalcium phosphate (TCP) on Pikovskaya (PVK) solid medium. Out of 29 phosphate solubilizing bacteria (PSB), 5 isolates were selected for their solubilization diameters (between 0.6 and 1.5 cm). These isolates were characterized for plant growth promoting (PGP) traits. The results showed that the highest concentration of indole acetic acid (IAA) was produced by LCR33 (19.08 ± 0.96 mg L-1). All 5 isolates could produce hydrogen cyanide (HCN), siderophores, ammonia and amino-cyclopropane carboxylate (ACC) deaminase. The isolates were evaluated for TCP solubilizing quantitative assay in PVK liquid medium. The concentrations of solubilized P were between 43.34±0.18 mg L-1 and 173.57±0.77 mg L-1. This solubilization was accompanied by a pH decrease of the culture media from 7 to 4.06. Furthermore, the 5 selected PSB were tested in vitro for antagonism against phytopathogenic fungus Fusarium oxysporum. In fact, all the PSB, were capable of inhibiting its growth and the highest percentages of inhibition were obtained for LCP27 and LCR33 (48.15±0.99% and 40.74±0.45%). Also, the effect of these 2 PSB on growth of L. creticus plants was investigated under greenhouse conditions. Significant increases were obtained for shoot and root length and dry and fresh matter production of plants as compared to the uninoculated control. These PSB could be recommended as biofertilizers for contributing to the rehabilitation of degraded soils.

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Cited by 192 publications

References 36 publications

Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion

Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion

Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

Screening for Lotus creticus growth promoting rhizobacteria under greenhouse conditions

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