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Thảo, Nguyễn Thị Phương; Ureshino, Kenji; Miyajima, Ikuo; Ozaki, Yukio; Okubo, Hiroshi

doi:10.1023/a:1021292928295

Cited by 136 publications

(25 citation statements)

References 19 publications

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“…In this experiment, we found that the maximum production of tetraploids was about 7.6%, which compared favorably with percentages reported for Zizyphus jujuba Mill. (5.0%) (Gu et al 2005) and Alocasia (10.5%) (Thao et al 2003). Rapid propagation of Anthurium andraeanum from regenerable masses has been successfully achieved for many popular cultivated varieties, including Fire, Alabama, Vitara and Pink Champion.…”

Section: Discussionmentioning

confidence: 99%

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Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

et al. 2011

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Tetraploids plants of Anthurium andraeanum ''Arizona'' were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7 h, and then were transferred into Murashige and Skoog medium containing 3 mg/l BAP ? 0.2 mg/l 2,4-D. After 60 days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation.

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Section: Discussionmentioning

confidence: 99%

“…Among others, induction of polyploidy has been achieved in many Araceae members, such as Xanthosoma (Esnard et al 1993), Zantedeschia , Alocasia (Thao et al 2003), Spathiphyllum wallisii Regel (Eeckhaut et al 2004), Zamioculcas Zamiifolia (Vanzie-Canton and Leonhardt 2009).…”

Section: Introductionmentioning

confidence: 99%

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

et al. 2011

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“…In the breeding field, ploidy manipulation is a valuable tool for crop quality improvement; and polyploidy, especially tetraploidy induction has been adopted as an efficient breeding strategy [1,2]. Failure of chromosomes or chromatids to separate properly to opposite poles during meiosis or mitosis in above polyploidy often results in the occurrence of aneuploidy.…”

Section: Introductionmentioning

confidence: 99%

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

Jiang

Ren

et al. 2017

Acta Soc Bot Pol

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Aneuploidy often presents large variations in morphology, physiology, biochemistry, and genetics owing to karyotypic imbalance. This study aimed to evaluate the efficacy of aneuploid breeding in Echinacea purpurea L, an important medicinal plant. Reciprocal crosses between diploid and triploid plants were performed to generate aneuploid plants. Cross with triploid as female parent resulted in increased production of aneuploid individuals (19 of 23; 82.61%), while using diploid as female parent yielded much higher percentage of diploid progenies (130 of 133; 97.74%). Each aneuploid had particular karyotypic characteristics compared to the parents. The proportions of median, submedian, and subterminal centromere location chromosomes in gross chromosomes among aneuploids and two parents showed large variations. Although aneuploids had relatively lower adventitious bud regeneration rates than their parents, almost half of them looked morphologically normal, with high survival rates when transplanted to ex vitro conditions. Among the bioactive compounds assessed, cichoric acid and chlorogenic acid contents were extremely encouraging. Most aneuploids had higher cichoric acid and chlorogenic acid contents than their parents. For example, A2 had the highest cichoric acid content of 21.98 mg/g dry weight, more than twice the values of diploid and triploid. Meanwhile, A21 had the highest chlorogenic acid content of 1.84 mg/g, approximately five times more than the parental values. Eleven superior aneuploid lines were successfully screened as breeding candidates. The present findings indicated E. purpurea is highly tolerant of karyotypic imbalance and aneuploid plants could serve as prospective breeding resources in E. purpurea.

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Section: Introductionmentioning

confidence: 99%

“…Ramsey and Schemske estimated its frequency at 0.56% [7]. Although with the use of colchicine the frequency of the somatic doubling has increased a lot, the effective diploidization rate is still very low (10.5%) [8].In addition, problems with chimeras, abnormal phenotypes and sterility also occur [9]. Little attention, however, has been focused on the use of this method, although a "synthetic" allotetraploid had been obtained by crossing a tetraploid Arabidopsis thaliana (2n = 4x = 20) and A. arenosa (2n = 4x = 32) [10].…”

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confidence: 99%

Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

Sun

Zhang

et al. 2014

Acta Soc Bot Pol

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IntroductionIt is estimated that 47% of all flowering plants and 95% of all pteridophytes are polyploids and that the majority of these are allopolyploids [1,2], which is a widespread and major force of evolution in plants [3]. Allopolyploidy is often accompanied by major structural, cytogenetic, epigenetic and functional changes to the genome, leading to new phenotypes and to reproductive isolation [4,5]. In addition, the permanent heterozygosity fixation of the allopolyploid [6] has the potential to offer a substantial heterozygote advantage. Despite these potential benefits, allopolyploid is an enormous challenge with the orchestration of gene expression, DNA replication, and chromosome pairing. For these reasons, investigation of allopolyploids is very important. The newly synthesized allopolyploid is an ideal model system since it can offer an opportunity to study the response to this genomic change from defined parents.Allopolyploid formation can occur by two main pathways, the so-called "one-step" and "two-step" models [5]. In the one-step model, the allopolyploid arises directly from an interspecific cross by the fusion of either two unreduced (2n) gametes from diploid parents or two normal (n = 2x) gametes from tetraploid parents. By contrast, in the two-step model, an interspecific F1 hybrid is first formed and the polyploidy is derived from it either from a fertile shoot generated by meristematic tissues having experienced a somatic doubling or by fusion of two 2n gametes produced by the F1 hybrid itself [6]. The production of 2n gametes appears to occur at a surprisingly low rate. Ramsey and Schemske estimated its frequency at 0.56% [7]. Although with the use of colchicine the frequency of the somatic doubling has increased a lot, the effective diploidization rate is still very low (10.5%) [8].In addition, problems with chimeras, abnormal phenotypes and sterility also occur [9]. Little attention, however, has been focused on the use of this method, although a "synthetic" allotetraploid had been obtained by crossing a tetraploid Arabidopsis thaliana (2n = 4x = 20) and A. arenosa (2n = 4x = 32) [10].In the crop Brassica, breeders have resorted to varying degrees of hybridization involving close relatives of it in their search for novel traits in developing new and improved varieties [11]. The non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is a main vegetable, which grows in south of China, and it has a long history of cultivation in our country. Radish (Raphanus sativus L.) is cultivated worldwide. It possesses desirable agronomic characters, such as resistance to white rust (Albugo candida) [12], BCN (Heterodera schachtii) [13,14] and culbroot (Plasmodiophora brassicae) [15], as well as resistance to pod shattering [16]. Besides, various related wild species have attracted research attention as potential germplasms AbstractIntergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x ...

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Cited by 136 publications

References 19 publications

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

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