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Abstract

Canine adenovirus type 1 (CAV-1) and type 2 (CAV-2) can be categorized in the laboratory by haemagglutination and neutralization tests, but they are difficult to differentiate from each other in specimens, especially when infection occurs in the digestive tract. The object of this study was to develop a simple method of detecting and differentiating them. One pair of common primers was designed and synthesized according to the sequences of the E3 and flanking regions and a polymerase chain reaction (PCR) assay was established using these two primers to amplify the virus-specific DNA fragment from clinical specimens as well as from cell cultures. After elecctrophoresis, under the same amplification conditions, 508 bp and 1030 bp PCR products were observed for CAV-1 and CAV-2, respectively. These were further shown to be adenovirus specific by dot hybridization and sequencing. As only one pair of primers was involved in the PCR procedure, it was faster and easier to perform than any of the other assays used for detecting canine adenovirus, making it applicable in the rapid confirmation of diagnosis and differentiation of the two types of canine adenoviruses.

Genome Sequence of Canine Adenovirus Type 1 Isolated from a Wolf ( Canis lupus ) in Southern Italy

“…DNA extracted from liver homogenate tested positive for CAdV-1 by conventional PCR (6); the homogenate was subsequently used for viral isolation on MDCK cells. CAdV-1 ITL2015 was successfully isolated at the third cell passage.…”

An outbreak of fatal hemorrhagic pneumonia caused by Streptococcus equi subsp. zooepidemicus in shelter dogs

“…Antimicrobial susceptibility test was performed by the disc diffusion method using 20 antimicrobial drugs. For viral agents, PCR was carried out to amplify the specific sequences of the canine distemper (CD) and canine adenovirus type 1 and 2 (CAV-1, CAV-2) using methods described previously by Elia et al [5] and Hue et al [6], respectively. Canine parainfluenza virus (CPIV) was examined by a commercial kit (Veteck CPIV; Intron, Korea).…”

Detection and molecular characterization of canine adenovirus type 2 (CAV-2) in dogs with respiratory tract symptoms in shelters in Turkey

“…Extraction was carried out according to the manufacturer's instructions. Primers and methods suitable for both CAV-1 and CAV-2 were used for the PCR, as recommended by HU et al, (2001) andCHATURVEDI et al (2008). Consequently, the formation of PCR products in the expected sizes (508 bp and 1030 bp for CAV-1 and CAV-2, respectively) was analysed using DNA gel electrophoresis.…”

“…CAV-1 targets the digestive tract tissue, and causes hepatitis contagiousa canis infection by damaging the liver. CAV-2 targets lung tissue where it causes infectious laryngotracheitis (HU et al, 2001).…”

“…Although various methods, such as virus isolation, haemaglutination, and neutralization, can be utilised for the diagnosis of adenoviruses, factors such as time, labour, and the systems affected by the infections (HU et al, 2001) have recently increased the preference for polymerase chain reaction (PCR)-based methods (SILVA et al, 2014;BOOMKENS et al, 2005;DECARO et al, 2007). The CAV-2 genome consists of gene regions that encode various proteins with specific functions.…”