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Adelberg, Jeffrey; Fujiwara, Kazuhiro; Kirdmanee, Chalermpol; Kozai, Toyoki

doi:10.1023/a:1006317130744

Cited by 13 publications

(2 citation statements)

References 15 publications

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“…PR has been used in the root induction in commercial vegetative propagation since the laborious step of removing media from plantlet roots is not needed again [22,30]. More importantly, practices have demonstrated that PR produces better-developed plantlets with higher survival rates than conventional media-based rooting [31].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon

Wang

et al. 2019

IJMS

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Agrobacterium-mediated genetic transformation is well established in the model grass Brachypodium distachyon. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with the immature embryo system is that they are available only during a limited time window of growing plants. In this study, we have developed an optimized Agrobacterium-mediated genetic transformation protocol that utilizes mature embryos. We have adopted seed shearing and photoautotrophic rooting (PR) in callus induction and root regeneration, respectively, with evident significant improvement in these aspects. We have also revealed that the newly developed chemical inducer Fipexide (FPX) had the ability to induce callus, shoots, and roots. By comparison, we have demonstrated that FPX shows higher efficiency in shoot generation than other frequently used chemicals in our mature embryo-based system. In addition, we demonstrated that the age of embryogenetic callus severely affects the transformation efficiency (TE), with the seven-week-old embryogenetic callus having the highest TE reaching 52.6%, which is comparable with that in immature embryo transformation. The new methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.

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Section: Discussionmentioning

confidence: 99%

Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Nu«i cÊy m« trªn m«i tr−êng kh«ng ®−êng ®• c ¶i tiÕn ®−îc sù sinh tr−ëng cña c©y in vitro, gi ¶m ®−îc tû lÖ t¹p nhiÔm vµ chi phÝ ch¨m sãc trong nhµ −¬m [9,10]. Mét sè nghiªn cøu kh¸c vÒ sù bae sung gi¸ thÓ vµ sù trao ®aei khÝ còng ®• c ¶i tiÕn ®−îc sù ph¸t triÓn cña hÖ thèng cña rÔ c©y cÊy m« [11][12][13][14][15]. Nh÷ng nghiªu cøu trªn ®• më ra mét ý t−ëng cÇn mét hÖ thèng nh©n gièng v« tÝnh in vitro nh»m t¹o ra c©y d©u t©y cã chÊt l−îng cao, ®ång thêi gi ¶m tû lÖ chÕt cña c©y con ë giai ®o¹n ex vitro.…”

Section: Viön Sinh Häc Nhiöt ®íIunclassified