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Bate, Neil; Twell, David

doi:10.1023/a:1006095023050

Cited by 220 publications

(42 citation statements)

References 29 publications

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“…GTGA motifs have been identiWed in the promoter of the tobacco late pollen gene g10, and shown to be important in directing high levels of pollen-speciWc expression of the gene (Twell et al 1991;Rogers et al 2001). POLLEN1LELAT52 (AGAAA) is one of two codependent regulatory elements responsible for pollenspeciWc activation of the tomato LAT52 gene (Bate and Twell 1998;Filichkin et al 2004). GTGA and AGAAA sequences are also present in the 5Ј Xanking region of RTS, an anther-speciWc gene isolated from rice.…”

Section: Discussionmentioning

confidence: 99%

“…A search for cisacting elements in this putative Osg1 promoter (¡870 to +131) using plant cis-acting regulatory DNA elements (http://www.dna.affrc.go.jp/htdocs/PLACE/; Higo et al 1999) indicated that this putative promoter contained several anther/pollen-speciWc regulatory motifs. There are Wve copies of the "GTGA motif" (Rogers et al 2001), two copies of the POLLEN1LELAT52 motifs (AGAAA) (Bate and Twell 1998;Filichkin et al 2004), and a QELEM ENTZMZM13 motif (AGGTCA) (Hamilton et al 1998) (Fig. 1i).…”

Section: Analysis Of Osg1 Expression In Ricementioning

confidence: 99%

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A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

Wan

Zha

Cheng

et al. 2010

Planta

123

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Plant β-1,3-glucanases are involved in plant defense and development. In rice (Oryza sativa), 14 genes encoding putative β-1,3-glucanases have been isolated and sequenced. However, only limited information is available on the function of these β-1,3-glucanase genes. In this study, we report a detailed functional characterization of one of these genes, Osg1. Osg1 encodes a glucanase carrying no C-terminal extension. Osg1 was found to be expressed throughout the plant and highly expressed in florets, leaf sheaths, and leaf blades. Investigations using real-time PCR, immunocytochemical analysis, and a GUS-reporter gene driven by the Osg1 promoter indicated that Osg1 was mainly expressed at the late meiosis, early microspore, and middle microspore stages in the florets. To elucidate the role of Osg1, we suppressed expression of the Osg1 gene by RNA interference in transgenic rice. The silencing of Osg1 resulted in male sterility. The pollen mother cells appeared to be normal in Osg1-RI plants, but callose degradation was disrupted around the microspores in the anther locules of the Osg1-RI plants at the early microspore stage. Consequently, the release of the young microspores into the anther locules was delayed, and the microspores began to degenerate later. These results provide evidence that Osg1 is essential for timely callose degradation in the process of tetrad dissolution.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Osg1 Expression In Ricementioning

confidence: 99%

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

Wan

Zha

Cheng

et al. 2010

Planta

123

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“…The Cry j 2 promoter sequences contained the cis- elements responsible for expression in pollen, namely LAT52 [27], the core motifs of LAT52/56 and LAT56/59 boxes [27, 28], NTP303 [29], and the enhancing Q (quantitative) element [30] (fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Genes for a Pollen Allergen, Cry j 2, of <i>Cryptomeria japonica</i>

Futamura

Kusunoki²,

Mukai³

et al. 2006

Int Arch Allergy Immunol

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Background: Cry j 2 is one of the major pollen allergens of Cryptomeria japonica. The polymorphism of Cry j 2 isoforms and the conservation of the structure of Cry j 2 in coniferous species remain to be analyzed. Methods:A cDNA library derived from the pollen of C. japonica was screened using a fragment of Cry j 2 cDNA. Restriction fragment length polymorphism analysis was performed to examine the diversity of Cry j 2 genes. The promoters of Cry j 2 genes were isolated with a commercially available cloning kit. Clonal variations in the expression of Cry j 2 in pollen were examined by RNA gel blot analysis, and the conservation of the structure of the Cry j 2 gene in coniferous species was evaluated by DNA gel blot analysis. Results: We isolated three cDNA clones encoding novel isoforms of Cry j 2. We also sequenced a total of 16 promoter regions from 10 specimens. The sequences of promoter regions of Cry j 2 genes were highly divergent. The amount of Cry j 2 mRNA also varied considerably. The Cry j 2 gene was found to be conserved among species belonging to Taxodiaceae and Cupressaceae but to vary between Taxodiaceae and Pinaceae. Conclusions: The coding and promoter regions of Cry j 2 genes contain large numbers of polymorphisms. Our analysis revealed large variations in the expression of Cry j 2 at the transcriptional level, and we suggest that conserved homologs of Cry j 2 confer cross-allergenicity among Taxodiaceae and Cupressaceae.

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“…Structural analysis of the Bet v 1a proximal promoter using network software (see Materials and Methods) identified putative CAAT and TATA boxes and other general regulatory elements modulating gene expression such as Box II [38]and GT-1 [39]. Furthermore, the Bet v 1a proximal promoter sequence contains elements responsible for expression in pollen, namely LAT52 [40], GTGA [41], and the enhancing Q (quantitative) element [42](fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…However, the widespread and successful use of tobacco to study the activities of promoters isolated from other species, e.g. carrot, tomato, maize and rapeseed [36, 40, 51, 52, 53, 54], convinced us that the proposed model covers the main aspects of the environment-dependent induction of the Bet v 1a gene.…”

Section: Discussionmentioning

confidence: 99%

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

Tashpulatov¹,

Clement²,

Akimcheva³

et al. 2004

Int Arch Allergy Immunol

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Background: The major birch pollen allergen Bet v 1 (or Bet v 1a) is one of the main causes of seasonal type I allergies. Various environmental factors such as light, temperature and air pollution may influence the activity of the Bet v 1a gene. The creation of a model system to evaluate the role of environmental factors affecting the Bet v 1a gene expression would be highly desirable. We suggest the use of transgenic tobacco plants carrying a Bet v 1a promoter-reporter gene fusion as such a system. Methods: The promoter of the Bet v 1a gene was isolated with the use of the Universal Genome Walker kit (BD Biosciences Clontech, USA). Web Software was used to search for putative cis-regulatory elements within the promoter. Transgenic tobacco plants harboring the promoter-β-glucuronidase (GUS) reporter gene fusion were obtained via Agrobacterium tumefaciens-mediated transformation. Promoter activity was examined with histochemical and quantitative assays. Results: Structural analysis predicted elements responsible for pollen-specific, light-, stress- and hormone-mediated induction within the Bet v 1a promoter. The evaluation of GUS activity in transgenic tobacco plants showed that the Bet v 1a promoter is pollen-specific. Moreover, the Bet v 1a promoter is considered to be the strongest isolated pollen-specific promoter reported to date. It was shown that temperature and abscisic acid positively regulate the activity of the Bet v 1a promoter during pollen development, providing evidence for environment-dependent regulation of the Bet v 1a gene. Conclusions: A model system to study the effect of environmental factors on the expression of the Bet v 1a gene encoding the major birch allergen in pollen was generated. Additionally, we suggest that this system could be used to search for factors that inhibit the activity of the gene in pollen in order to reduce the potential allergenicity of birch trees.

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Untitled

Cited by 220 publications

References 29 publications

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

Characterization of Genes for a Pollen Allergen, Cry j 2, of <i>Cryptomeria japonica</i>

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

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