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Effect of adrenaline on the functions of rat peripheral blood phagocytes under stress and upon hydrocortisone administration

“…Summarizing our data and the results obtained earlier [1][2][3][6][7][8], it is possible to conclude that the stimulatory effect of catecholamines on the oxygen-dependent bactericide activity of phagocytes is mediated by α-adrenoreceptors. The administration of hydrocortisone completely eliminated the activating effect of adrenaline (p > 0.05, data not shown), which may be due to the hormone-induced enhancement of the expression of β-adrenoreceptors, because the blockage of β-adrenoreceptors under these conditions led again to the stimulatory effect of adrenaline in vitro (the extinctions in the experiment and control were, respectively, 0.44 ± 0.05 and 0.35 ± 0.03; p < 0.05).…”

“…Earlier, we showed that acute immobilization stress and treatment with hydrocortisone enhanced the phagocytic activity of peripheral blood leukocytes [1][2][3]. It is well known that glucocorticoid hormones have a permissive effect on catecholamines accounted for by the enhancement of the expression of adrenoreceptors in target cells of different organs [4,5].…”

“…Doses and schemes of drug introduction were based on the results of previous studies [9,10]. Samples of peripheral blood were taken from tail vessels of intact animals (the combined background) 6 and 24 h after the beginning of the experiment, i.e., when the changes were especially pronounced, according to earlier data [1][2][3].…”

“…In control samples, BM was added instead of the preparation. The total phagocytic activity of leukocytes was studied in comparison with formalin-treated sheep erythrocytes of sheep as described earlier [1,2]. The oxygen-dependent bactericide activity of peripheral blood leukocytes was estimated by a modified spectrophotometric test with tetrazolium nitroblue (TNB test) [3,11].…”