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Zhou, Shengde; Ingram, L. O.

doi:10.1023/a:1011623509335

Cited by 75 publications

(6 citation statements)

References 23 publications

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“…Microbacterium species produced enzymes involved in cellulose and xylan degradation [62][63][64][65]. Recombinant strains of Klebsiella oxytoca have been employed in cellulose degradation and fermentation [66,67]. However, there is paucity of information on lignocellulose biodegradation by natural isolates of Klebsiella.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Okeke

2010

Appl Biochem Biotechnol

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Diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. Inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. In an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, purified 19 monocultures and evaluated their cellulolytic-xylanolytic potential. Five selected isolates (DB1, DB2, DB7, DB8, and DB13) were used to compose a defined consortium and characterized by 16S ribosomal RNA gene sequence analysis. Nucleotide sequence blast analysis revealed that DB1, DB2, DB7, DB8, and DB13 were respectively similar to Pseudoxanthomonas byssovorax (99%), Microbacterium oxydans (99%), Bacillus sp. (99%), Ochrobactrum anthropi (98%), and Klebsiella trevisanii (99%). The isolates produced an array of cellulolytic-xylanolytic enzymes (filter paper cellulase, β-glucosidase, xylanase, and β-xylosidase), and significant activities were recorded in 30 min. Isolates DB1 and DB2 displayed the highest filter paper cellulase: 27.83 and 31.22 U mg⁻¹, respectively. The highest β-glucosidase activity (18.07 U mg⁻¹) was detected in the culture of isolate DB1. Isolate DB2 produced the highest xylanase activity (103.05 U mg⁻¹), while the highest β-xylosidase activity (7.72 U mg⁻¹) was observed with DB13. Use of microbial consortium in bioprocessing of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, adsorption, and requirement for high amounts of enzymes in direct use of enzymes.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Okeke

2010

Appl Biochem Biotechnol

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“…As described above, K. oxytoca was engineered for ethanol production. The native cellobiose-depolymerization activity of this organism was expanded by expression of cellulase from Clostridium thermocellum (Wood & Ingram, 1992 ), and E. chrysanthemi endoglucanase (Zhou & Ingram, 1999 ; Zhou & Ingram, 2001 ). Ultimately, the Ingram group shifted to the use of abiotic biomass depolymerization in conjunction with commercial enzyme supplementation, as described below.…”

Section: Direct Biological Utilization Of Biomass and Plasticmentioning

confidence: 99%

Extrapolation of design strategies for lignocellulosic biomass conversion to the challenge of plastic waste

Jarboe

Khalid

Ocasio

et al. 2022

Journal of Industrial Microbiology and Biotechnology

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The goal of cost-effective production of fuels and chemicals from biomass has been a substantial driver of the development of the field of Metabolic Engineering. The resulting design principles and procedures provide a guide for the development of cost-effective methods for degradation, and possibly even valorization, of plastic wastes. Here we highlight these parallels, using the creative work of Lonnie O'Neal (Neal) Ingram in enabling production of fuels and chemicals from lignocellulosic biomass, with a focus on ethanol production as an exemplar process.

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“…Samples were spotted onto silica gel 60 TLC plates (20 cm by 20 cm; Millipore). Reaction products were separated by ascension with 150 ml of developing solvent (chloroform-acetic acid-water; 6:7:1 [vol/vol/vol]) (30), allowing the solvent to migrate to within 1 cm of the top of the plate. Plates were allowed to dry prior to a second ascension.…”

Section: Figmentioning

confidence: 99%

Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides

Rhee

Sawhney

et al. 2014

Appl Environ Microbiol

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cXylans are the predominant polysaccharides in hemicelluloses and an important potential source of biofuels and chemicals. The ability of Bacillus subtilis subsp. subtilis strain 168 to utilize xylans has been ascribed to secreted glycoside hydrolase family 11 (GH11) and GH30 endoxylanases, encoded by the xynA and xynC genes, respectively. Both of these enzymes have been defined with respect to structure and function. In this study, the effects of deletion of the xynA and xynC genes, individually and in combination, were evaluated for xylan utilization and formation of acidic xylooligosaccharides. Parent strain 168 depolymerizes methylglucuronoxylans (MeGX n ), releasing the xylobiose and xylotriose utilized for growth and accumulating the aldouronate methylglucuronoxylotriose (MeGX 3 ) with some methylglucuronoxylotetraose (MeGX 4 ). The combined GH11 and GH30 activities process the products generated by their respective actions on MeGX n to release a maximal amount of neutral xylooligosaccharides for assimilation and growth, at the same time forming MeGX 3 in which the internal xylose is substituted with methylglucuronate (MeG). Deletion of xynA results in the accumulation of ␤-1,4-xylooligosaccharides with degrees of polymerization ranging from 4 to 18 and an average degree of substitution of 1 in 7.2, each with a single MeG linked ␣-1,2 to the xylose penultimate to the xylose at the reducing terminus. Deletion of the xynC gene results in the accumulation of aldouronates comprised of 4 or more xylose residues in which the MeG may be linked ␣-1,2 to the xylose penultimate to the nonreducing xylose. These B. subtilis lines may be used for the production of acidic xylooligosaccharides with applications in human and veterinary medicine.

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Cited by 75 publications

References 23 publications

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses

Extrapolation of design strategies for lignocellulosic biomass conversion to the challenge of plastic waste

Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides

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