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Зленко, Валерий Анатольевич; Kotikov, I. V.; Troshin, L. P.

doi:10.1023/a:1016593227463

Cited by 16 publications

(7 citation statements)

References 18 publications

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“…It has antioxidant properties, promotes the development of normal tissues (vessels and grains of birch and pine), while the activity of peroxidase and polyphenol oxidase is 3, 7 and 2.3 times higher, respectively, in woods containing parenchymal cells. Therefore, DLPA inhibited the formation of proembryogenic undifferentiated cells, but promoted the development of various stages of somatic embryoids and seedlings [6,10] In the process of a long-term subculturing of suspensions of proembryogenic cells and embryoids due to somaclonal variability, it was not possible to get rid of chlorophyll deficiency, i.e. the presence of white cotyledons in germinating seedlings resulted from embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Suspension culture with small globular embryoids (<0.2 mm) (about 1000 pcs. of 0.05-0.2 mm in size in 10 ml of suspension) was equally added to culture vessels, in which different variants of nutrient media with the base, contained fourfold diluted mineral elements and vitamins of PG medium [10], and 10 g/l of sucrose were added. The variants of media differed in the presence or absence of growth regulators and biologically active substances.…”

Section: Methodsmentioning

confidence: 99%

“…of the developed somatic embryoids and germinating seedlings (y1...y4), and by the size of seedlings (mm, y5,). The conditions for conducting of the experiments were presented earlier [10].…”

Section: Methodsmentioning

confidence: 99%

“…But if somatic embryogenesis in the culture in vitro comes easily in some plant families and species, then in others it is strictly molecularly and genetically determined, and affected by many factors [7,9]. The induction of somatic embryogenesis in plants of different species and in grapes from cells of suspension cultures was achieved in a small number of varieties, creating difficulties for application of modern biotechnology methods [7,8,10,11].…”

Section: Introductionmentioning

confidence: 99%

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Features of somatic embryogenesis in the culture in vitro in hybrid grape form E-342

et al. 2022

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Correlation analysis of the influence of media variants with introducing various biologically active substances on the development of somatic embryoids and germinating seedlings in the hybrid form E-342 was carried out during two repeated subculturings on the same variant of media, and during the third subculturing - on different variants of media. The repeated subculturing in media with the same growth regulator BAP or IAA caused the development of abnormal large somatic embryoids without the expressed hypocotyls and cotyledons. Only the use of medium variant with DLPA in two subculturings (the third subculturing into the medium with BAP or IAA) and the third repeated subculturing with DLPA, but to a smaller extent, have led to the development of torpedo-shaped embryoids, to be growing into germinating seedlings. Strong positive correlation dependence of influence was established for: BAP on the development of heart-shaped embryoids; IAA on the increase in the size of globular embryoids and transformation of heart-shaped embryoids into torpedo-shaped ones; DLPA on the development of a great number of normal torpedo-shaped embryoids. After longstanding culturing of suspensions of cells and embryoids, it was not possible to get rid of chlorophyll deficiency in the resulting germinating seedlings due to somaclonal variability.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Features of somatic embryogenesis in the culture in vitro in hybrid grape form E-342

et al. 2022

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“…The plant samples were propagated by micrografting. They were cultured for four weeks on a hormone-free PG medium, at a 16-hour photoperiod; the temperature was +25-27 o C [11]. Afterwards, plant containing culture bottles were in the cold storage without internal lighting at 10-12°C for 6 months.…”

Section: Methodsmentioning

confidence: 99%

The effect of cultivation conditions on the growing processes of grape plants in vitro

et al. 2021

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One of alternative ways to maintain valuable genetic material is to develop optimal conditions for cultivation in an in vitro system. The goal was to evaluate the condition of plants based on changes in the shoot length indicator in order to explore the maintaining mode of the collection. We took the experimental samples from the “Vegetating collection of plants in vitro of promising varieties and clones of grapes”: 13 local Crimean varieties and 9 varieties of the Institute Magarach selection. Plant containing culture bottles were in the cold storage without internal lighting at 10-12°C for 6 months. The cultivation findings demonstrated that grape varieties differed in viability and intensity of morphogenesis. Moreover, after storage, they had a high regenerating ability of the buds.

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